Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11125 and RR42_RS11130 are separated by 74 nucleotides RR42_RS11130 and RR42_RS11135 overlap by 1 nucleotides RR42_RS11135 and RR42_RS11140 overlap by 4 nucleotides
RR42_RS11125: RR42_RS11125 - glycerophosphodiester phosphodiesterase, at 2,375,845 to 2,377,218
_RS11125
RR42_RS11130: RR42_RS11130 - mannosyltransferase, at 2,377,293 to 2,378,006
_RS11130
RR42_RS11135: RR42_RS11135 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,378,006 to 2,378,779
_RS11135
RR42_RS11140: RR42_RS11140 - ABC transporter permease, at 2,378,776 to 2,380,089
_RS11140
Position (kb)
2377
2378
2379 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2376.361 kb on + strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125 at 2376.931 kb on + strand, within RR42_RS11125 at 2376.932 kb on - strand, within RR42_RS11125 at 2377.216 kb on + strand at 2377.217 kb on - strand at 2377.294 kb on + strand at 2377.295 kb on - strand at 2377.472 kb on - strand, within RR42_RS11130 at 2377.611 kb on - strand, within RR42_RS11130 at 2377.645 kb on + strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.759 kb on + strand, within RR42_RS11130 at 2377.925 kb on + strand, within RR42_RS11130 at 2377.950 kb on - strand at 2377.967 kb on - strand at 2378.052 kb on + strand at 2378.331 kb on + strand, within RR42_RS11135 at 2378.332 kb on - strand, within RR42_RS11135 at 2378.356 kb on + strand, within RR42_RS11135 at 2378.357 kb on - strand, within RR42_RS11135 at 2378.445 kb on + strand, within RR42_RS11135 at 2378.446 kb on - strand, within RR42_RS11135 at 2378.512 kb on + strand, within RR42_RS11135 at 2378.512 kb on + strand, within RR42_RS11135 at 2378.513 kb on - strand, within RR42_RS11135 at 2378.654 kb on - strand, within RR42_RS11135 at 2378.654 kb on - strand, within RR42_RS11135 at 2378.750 kb on + strand at 2378.751 kb on - strand at 2378.751 kb on - strand at 2378.807 kb on - strand at 2378.972 kb on + strand, within RR42_RS11140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 2,376,361 + RR42_RS11125 0.38 +0.2 2,376,362 - RR42_RS11125 0.38 +0.2 2,376,362 - RR42_RS11125 0.38 +0.2 2,376,931 + RR42_RS11125 0.79 -0.0 2,376,932 - RR42_RS11125 0.79 -0.8 2,377,216 + +1.9 2,377,217 - +0.2 2,377,294 + -1.8 2,377,295 - +0.9 2,377,472 - RR42_RS11130 0.25 -0.3 2,377,611 - RR42_RS11130 0.45 -0.7 2,377,645 + RR42_RS11130 0.49 -0.6 2,377,646 - RR42_RS11130 0.49 -0.3 2,377,646 - RR42_RS11130 0.49 +0.1 2,377,646 - RR42_RS11130 0.49 -0.9 2,377,646 - RR42_RS11130 0.49 +0.1 2,377,646 - RR42_RS11130 0.49 +0.0 2,377,646 - RR42_RS11130 0.49 -0.1 2,377,646 - RR42_RS11130 0.49 -0.2 2,377,740 - RR42_RS11130 0.63 -0.5 2,377,740 - RR42_RS11130 0.63 -0.5 2,377,759 + RR42_RS11130 0.65 +1.1 2,377,925 + RR42_RS11130 0.89 +0.5 2,377,950 - -0.1 2,377,967 - +0.7 2,378,052 + -1.4 2,378,331 + RR42_RS11135 0.42 +1.1 2,378,332 - RR42_RS11135 0.42 +0.4 2,378,356 + RR42_RS11135 0.45 -2.1 2,378,357 - RR42_RS11135 0.45 +0.8 2,378,445 + RR42_RS11135 0.57 -0.2 2,378,446 - RR42_RS11135 0.57 +0.5 2,378,512 + RR42_RS11135 0.65 -0.2 2,378,512 + RR42_RS11135 0.65 +0.4 2,378,513 - RR42_RS11135 0.66 -0.9 2,378,654 - RR42_RS11135 0.84 -0.1 2,378,654 - RR42_RS11135 0.84 -0.8 2,378,750 + +0.5 2,378,751 - +0.1 2,378,751 - +0.7 2,378,807 - +0.1 2,378,972 + RR42_RS11140 0.15 -2.7
Or see this region's nucleotide sequence