Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09075 and RR42_RS09080 are separated by 49 nucleotides RR42_RS09080 and RR42_RS09085 overlap by 1 nucleotides RR42_RS09085 and RR42_RS09090 are separated by 223 nucleotides
RR42_RS09075: RR42_RS09075 - amino acid dehydrogenase, at 1,909,075 to 1,910,373
_RS09075
RR42_RS09080: RR42_RS09080 - C4-dicarboxylate ABC transporter permease, at 1,910,423 to 1,911,733
_RS09080
RR42_RS09085: RR42_RS09085 - C4-dicarboxylate ABC transporter permease, at 1,911,733 to 1,912,284
_RS09085
RR42_RS09090: RR42_RS09090 - C4-dicarboxylate ABC transporter substrate-binding protein, at 1,912,508 to 1,913,467
_RS09090
Position (kb)
1910
1911
1912 Strain fitness (log2 ratio)
-2
-1
0
1 at 1909.448 kb on + strand, within RR42_RS09075 at 1909.514 kb on + strand, within RR42_RS09075 at 1909.515 kb on - strand, within RR42_RS09075 at 1909.530 kb on - strand, within RR42_RS09075 at 1909.831 kb on - strand, within RR42_RS09075 at 1910.034 kb on - strand, within RR42_RS09075 at 1910.034 kb on - strand, within RR42_RS09075 at 1910.222 kb on + strand, within RR42_RS09075 at 1910.296 kb on + strand at 1910.297 kb on - strand at 1910.321 kb on + strand at 1910.322 kb on - strand at 1910.383 kb on + strand at 1910.384 kb on - strand at 1910.389 kb on - strand at 1910.424 kb on + strand at 1910.544 kb on + strand at 1910.544 kb on + strand at 1910.544 kb on + strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.545 kb on - strand at 1910.549 kb on - strand at 1910.549 kb on - strand at 1910.639 kb on + strand, within RR42_RS09080 at 1910.651 kb on + strand, within RR42_RS09080 at 1910.652 kb on - strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.748 kb on + strand, within RR42_RS09080 at 1910.749 kb on - strand, within RR42_RS09080 at 1910.833 kb on + strand, within RR42_RS09080 at 1910.834 kb on - strand, within RR42_RS09080 at 1911.037 kb on + strand, within RR42_RS09080 at 1911.327 kb on + strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.557 kb on - strand, within RR42_RS09080 at 1912.094 kb on - strand, within RR42_RS09085 at 1912.152 kb on + strand, within RR42_RS09085 at 1912.392 kb on + strand at 1912.393 kb on - strand at 1912.413 kb on + strand at 1912.425 kb on + strand at 1912.461 kb on + strand at 1912.474 kb on - strand at 1912.519 kb on - strand at 1912.663 kb on - strand, within RR42_RS09090
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 1,909,448 + RR42_RS09075 0.29 +1.0 1,909,514 + RR42_RS09075 0.34 -0.5 1,909,515 - RR42_RS09075 0.34 +0.7 1,909,530 - RR42_RS09075 0.35 -0.2 1,909,831 - RR42_RS09075 0.58 +0.5 1,910,034 - RR42_RS09075 0.74 +0.6 1,910,034 - RR42_RS09075 0.74 +1.3 1,910,222 + RR42_RS09075 0.88 +0.2 1,910,296 + +0.3 1,910,297 - +0.3 1,910,321 + -2.0 1,910,322 - +1.0 1,910,383 + -2.5 1,910,384 - +0.1 1,910,389 - +0.3 1,910,424 + +1.7 1,910,544 + +0.7 1,910,544 + -0.3 1,910,544 + -0.1 1,910,545 - +0.3 1,910,545 - -0.1 1,910,545 - +1.7 1,910,545 - +0.4 1,910,545 - -0.6 1,910,549 - -1.1 1,910,549 - -0.9 1,910,639 + RR42_RS09080 0.16 -1.8 1,910,651 + RR42_RS09080 0.17 -1.1 1,910,652 - RR42_RS09080 0.17 -0.0 1,910,748 + RR42_RS09080 0.25 -1.1 1,910,748 + RR42_RS09080 0.25 +0.6 1,910,748 + RR42_RS09080 0.25 +1.3 1,910,748 + RR42_RS09080 0.25 +0.4 1,910,749 - RR42_RS09080 0.25 +0.0 1,910,833 + RR42_RS09080 0.31 +0.4 1,910,834 - RR42_RS09080 0.31 +1.1 1,911,037 + RR42_RS09080 0.47 -1.6 1,911,327 + RR42_RS09080 0.69 -2.3 1,911,516 - RR42_RS09080 0.83 +0.4 1,911,516 - RR42_RS09080 0.83 +0.9 1,911,516 - RR42_RS09080 0.83 -0.1 1,911,557 - RR42_RS09080 0.86 +1.3 1,912,094 - RR42_RS09085 0.65 +0.4 1,912,152 + RR42_RS09085 0.76 +1.5 1,912,392 + -0.8 1,912,393 - -0.6 1,912,413 + +0.4 1,912,425 + -0.9 1,912,461 + -0.6 1,912,474 - +0.5 1,912,519 - +0.7 1,912,663 - RR42_RS09090 0.16 +0.7
Or see this region's nucleotide sequence