Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS07435

Experiment: R2A with Nickel (II) chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS07430 and RR42_RS07435 are separated by 41 nucleotidesRR42_RS07435 and RR42_RS07440 overlap by 1 nucleotidesRR42_RS07440 and RR42_RS07445 are separated by 91 nucleotides RR42_RS07430: RR42_RS07430 - Fe-S oxidoreductase, at 1,589,139 to 1,589,864 _RS07430 RR42_RS07435: RR42_RS07435 - (Fe-S)-binding protein, at 1,589,906 to 1,591,315 _RS07435 RR42_RS07440: RR42_RS07440 - hypothetical protein, at 1,591,315 to 1,591,590 _RS07440 RR42_RS07445: RR42_RS07445 - ABC transporter ATP-binding protein, at 1,591,682 to 1,592,476 _RS07445 Position (kb) 1589 1590 1591 1592Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1589.039 kb on + strandat 1589.039 kb on + strandat 1589.040 kb on - strandat 1589.040 kb on - strandat 1589.040 kb on - strandat 1589.077 kb on + strandat 1589.077 kb on + strandat 1589.078 kb on - strandat 1589.103 kb on + strandat 1589.103 kb on + strandat 1589.103 kb on + strandat 1589.104 kb on - strandat 1589.104 kb on - strandat 1589.277 kb on + strand, within RR42_RS07430at 1589.277 kb on + strand, within RR42_RS07430at 1589.350 kb on - strand, within RR42_RS07430at 1589.350 kb on - strand, within RR42_RS07430at 1589.397 kb on + strand, within RR42_RS07430at 1589.535 kb on - strand, within RR42_RS07430at 1589.538 kb on + strand, within RR42_RS07430at 1589.538 kb on + strand, within RR42_RS07430at 1589.538 kb on + strand, within RR42_RS07430at 1589.538 kb on + strand, within RR42_RS07430at 1589.539 kb on - strand, within RR42_RS07430at 1589.539 kb on - strand, within RR42_RS07430at 1589.539 kb on - strand, within RR42_RS07430at 1589.683 kb on - strand, within RR42_RS07430at 1589.691 kb on - strand, within RR42_RS07430at 1589.819 kb on + strandat 1589.820 kb on - strandat 1589.834 kb on + strandat 1589.835 kb on - strandat 1589.906 kb on - strandat 1589.967 kb on + strandat 1589.968 kb on - strandat 1590.317 kb on + strand, within RR42_RS07435at 1590.318 kb on - strand, within RR42_RS07435at 1590.318 kb on - strand, within RR42_RS07435at 1590.707 kb on + strand, within RR42_RS07435at 1590.763 kb on + strand, within RR42_RS07435at 1590.763 kb on + strand, within RR42_RS07435at 1590.763 kb on + strand, within RR42_RS07435at 1590.763 kb on + strandat 1590.764 kb on - strand, within RR42_RS07435at 1590.764 kb on - strand, within RR42_RS07435at 1590.767 kb on + strand, within RR42_RS07435at 1590.767 kb on + strand, within RR42_RS07435at 1590.767 kb on + strand, within RR42_RS07435at 1590.767 kb on + strand, within RR42_RS07435at 1590.767 kb on + strand, within RR42_RS07435at 1590.767 kb on + strand, within RR42_RS07435at 1590.768 kb on - strand, within RR42_RS07435at 1590.768 kb on - strand, within RR42_RS07435at 1590.872 kb on + strand, within RR42_RS07435at 1590.945 kb on - strand, within RR42_RS07435at 1590.945 kb on - strand, within RR42_RS07435at 1591.143 kb on + strand, within RR42_RS07435at 1591.144 kb on - strand, within RR42_RS07435at 1591.145 kb on + strand, within RR42_RS07435at 1591.145 kb on + strand, within RR42_RS07435at 1591.146 kb on - strand, within RR42_RS07435at 1591.146 kb on - strand, within RR42_RS07435at 1591.146 kb on - strand, within RR42_RS07435at 1591.292 kb on + strandat 1591.293 kb on - strandat 1591.293 kb on - strandat 1591.293 kb on - strandat 1591.505 kb on + strand, within RR42_RS07440at 1591.519 kb on - strand, within RR42_RS07440at 1591.519 kb on - strand, within RR42_RS07440at 1592.100 kb on - strand, within RR42_RS07445

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM
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1,589,039 + -1.9
1,589,039 + +0.1
1,589,040 - +0.5
1,589,040 - +1.0
1,589,040 - +1.2
1,589,077 + -2.8
1,589,077 + +0.5
1,589,078 - +0.6
1,589,103 + +0.2
1,589,103 + -0.4
1,589,103 + -0.6
1,589,104 - -1.1
1,589,104 - -0.1
1,589,277 + RR42_RS07430 0.19 +0.3
1,589,277 + RR42_RS07430 0.19 -0.3
1,589,350 - RR42_RS07430 0.29 +0.9
1,589,350 - RR42_RS07430 0.29 +1.1
1,589,397 + RR42_RS07430 0.36 +0.9
1,589,535 - RR42_RS07430 0.55 +1.1
1,589,538 + RR42_RS07430 0.55 +0.8
1,589,538 + RR42_RS07430 0.55 +1.1
1,589,538 + RR42_RS07430 0.55 -0.8
1,589,538 + RR42_RS07430 0.55 -1.7
1,589,539 - RR42_RS07430 0.55 +0.1
1,589,539 - RR42_RS07430 0.55 +0.6
1,589,539 - RR42_RS07430 0.55 +2.3
1,589,683 - RR42_RS07430 0.75 +0.4
1,589,691 - RR42_RS07430 0.76 +0.2
1,589,819 + -0.5
1,589,820 - -1.2
1,589,834 + -0.2
1,589,835 - +0.1
1,589,906 - +1.4
1,589,967 + +0.3
1,589,968 - +1.6
1,590,317 + RR42_RS07435 0.29 +1.4
1,590,318 - RR42_RS07435 0.29 +0.7
1,590,318 - RR42_RS07435 0.29 +0.5
1,590,707 + RR42_RS07435 0.57 +0.5
1,590,763 + RR42_RS07435 0.61 -0.8
1,590,763 + RR42_RS07435 0.61 +0.5
1,590,763 + RR42_RS07435 0.61 +1.1
1,590,763 + -0.9
1,590,764 - RR42_RS07435 0.61 -0.3
1,590,764 - RR42_RS07435 0.61 +0.9
1,590,767 + RR42_RS07435 0.61 +2.8
1,590,767 + RR42_RS07435 0.61 +0.7
1,590,767 + RR42_RS07435 0.61 -1.3
1,590,767 + RR42_RS07435 0.61 -1.0
1,590,767 + RR42_RS07435 0.61 +0.6
1,590,767 + RR42_RS07435 0.61 +1.0
1,590,768 - RR42_RS07435 0.61 +1.8
1,590,768 - RR42_RS07435 0.61 +1.2
1,590,872 + RR42_RS07435 0.69 -0.0
1,590,945 - RR42_RS07435 0.74 -1.6
1,590,945 - RR42_RS07435 0.74 +1.2
1,591,143 + RR42_RS07435 0.88 -2.2
1,591,144 - RR42_RS07435 0.88 +1.3
1,591,145 + RR42_RS07435 0.88 +1.1
1,591,145 + RR42_RS07435 0.88 -0.6
1,591,146 - RR42_RS07435 0.88 +0.6
1,591,146 - RR42_RS07435 0.88 +0.7
1,591,146 - RR42_RS07435 0.88 +0.6
1,591,292 + -1.5
1,591,293 - +0.3
1,591,293 - -1.5
1,591,293 - +0.9
1,591,505 + RR42_RS07440 0.69 +0.4
1,591,519 - RR42_RS07440 0.74 +0.2
1,591,519 - RR42_RS07440 0.74 +1.0
1,592,100 - RR42_RS07445 0.53 -1.1

Or see this region's nucleotide sequence