Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07430 and RR42_RS07435 are separated by 41 nucleotides RR42_RS07435 and RR42_RS07440 overlap by 1 nucleotides RR42_RS07440 and RR42_RS07445 are separated by 91 nucleotides
RR42_RS07430: RR42_RS07430 - Fe-S oxidoreductase, at 1,589,139 to 1,589,864
_RS07430
RR42_RS07435: RR42_RS07435 - (Fe-S)-binding protein, at 1,589,906 to 1,591,315
_RS07435
RR42_RS07440: RR42_RS07440 - hypothetical protein, at 1,591,315 to 1,591,590
_RS07440
RR42_RS07445: RR42_RS07445 - ABC transporter ATP-binding protein, at 1,591,682 to 1,592,476
_RS07445
Position (kb)
1589
1590
1591
1592 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1589.039 kb on + strand at 1589.039 kb on + strand at 1589.040 kb on - strand at 1589.040 kb on - strand at 1589.040 kb on - strand at 1589.077 kb on + strand at 1589.077 kb on + strand at 1589.078 kb on - strand at 1589.103 kb on + strand at 1589.103 kb on + strand at 1589.103 kb on + strand at 1589.104 kb on - strand at 1589.104 kb on - strand at 1589.277 kb on + strand, within RR42_RS07430 at 1589.277 kb on + strand, within RR42_RS07430 at 1589.350 kb on - strand, within RR42_RS07430 at 1589.350 kb on - strand, within RR42_RS07430 at 1589.397 kb on + strand, within RR42_RS07430 at 1589.535 kb on - strand, within RR42_RS07430 at 1589.538 kb on + strand, within RR42_RS07430 at 1589.538 kb on + strand, within RR42_RS07430 at 1589.538 kb on + strand, within RR42_RS07430 at 1589.538 kb on + strand, within RR42_RS07430 at 1589.539 kb on - strand, within RR42_RS07430 at 1589.539 kb on - strand, within RR42_RS07430 at 1589.539 kb on - strand, within RR42_RS07430 at 1589.683 kb on - strand, within RR42_RS07430 at 1589.691 kb on - strand, within RR42_RS07430 at 1589.819 kb on + strand at 1589.820 kb on - strand at 1589.834 kb on + strand at 1589.835 kb on - strand at 1589.906 kb on - strand at 1589.967 kb on + strand at 1589.968 kb on - strand at 1590.317 kb on + strand, within RR42_RS07435 at 1590.318 kb on - strand, within RR42_RS07435 at 1590.318 kb on - strand, within RR42_RS07435 at 1590.707 kb on + strand, within RR42_RS07435 at 1590.763 kb on + strand, within RR42_RS07435 at 1590.763 kb on + strand, within RR42_RS07435 at 1590.763 kb on + strand, within RR42_RS07435 at 1590.763 kb on + strand at 1590.764 kb on - strand, within RR42_RS07435 at 1590.764 kb on - strand, within RR42_RS07435 at 1590.767 kb on + strand, within RR42_RS07435 at 1590.767 kb on + strand, within RR42_RS07435 at 1590.767 kb on + strand, within RR42_RS07435 at 1590.767 kb on + strand, within RR42_RS07435 at 1590.767 kb on + strand, within RR42_RS07435 at 1590.767 kb on + strand, within RR42_RS07435 at 1590.768 kb on - strand, within RR42_RS07435 at 1590.768 kb on - strand, within RR42_RS07435 at 1590.872 kb on + strand, within RR42_RS07435 at 1590.945 kb on - strand, within RR42_RS07435 at 1590.945 kb on - strand, within RR42_RS07435 at 1591.143 kb on + strand, within RR42_RS07435 at 1591.144 kb on - strand, within RR42_RS07435 at 1591.145 kb on + strand, within RR42_RS07435 at 1591.145 kb on + strand, within RR42_RS07435 at 1591.146 kb on - strand, within RR42_RS07435 at 1591.146 kb on - strand, within RR42_RS07435 at 1591.146 kb on - strand, within RR42_RS07435 at 1591.292 kb on + strand at 1591.293 kb on - strand at 1591.293 kb on - strand at 1591.293 kb on - strand at 1591.505 kb on + strand, within RR42_RS07440 at 1591.519 kb on - strand, within RR42_RS07440 at 1591.519 kb on - strand, within RR42_RS07440 at 1592.100 kb on - strand, within RR42_RS07445
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 1,589,039 + -1.9 1,589,039 + +0.1 1,589,040 - +0.5 1,589,040 - +1.0 1,589,040 - +1.2 1,589,077 + -2.8 1,589,077 + +0.5 1,589,078 - +0.6 1,589,103 + +0.2 1,589,103 + -0.4 1,589,103 + -0.6 1,589,104 - -1.1 1,589,104 - -0.1 1,589,277 + RR42_RS07430 0.19 +0.3 1,589,277 + RR42_RS07430 0.19 -0.3 1,589,350 - RR42_RS07430 0.29 +0.9 1,589,350 - RR42_RS07430 0.29 +1.1 1,589,397 + RR42_RS07430 0.36 +0.9 1,589,535 - RR42_RS07430 0.55 +1.1 1,589,538 + RR42_RS07430 0.55 +0.8 1,589,538 + RR42_RS07430 0.55 +1.1 1,589,538 + RR42_RS07430 0.55 -0.8 1,589,538 + RR42_RS07430 0.55 -1.7 1,589,539 - RR42_RS07430 0.55 +0.1 1,589,539 - RR42_RS07430 0.55 +0.6 1,589,539 - RR42_RS07430 0.55 +2.3 1,589,683 - RR42_RS07430 0.75 +0.4 1,589,691 - RR42_RS07430 0.76 +0.2 1,589,819 + -0.5 1,589,820 - -1.2 1,589,834 + -0.2 1,589,835 - +0.1 1,589,906 - +1.4 1,589,967 + +0.3 1,589,968 - +1.6 1,590,317 + RR42_RS07435 0.29 +1.4 1,590,318 - RR42_RS07435 0.29 +0.7 1,590,318 - RR42_RS07435 0.29 +0.5 1,590,707 + RR42_RS07435 0.57 +0.5 1,590,763 + RR42_RS07435 0.61 -0.8 1,590,763 + RR42_RS07435 0.61 +0.5 1,590,763 + RR42_RS07435 0.61 +1.1 1,590,763 + -0.9 1,590,764 - RR42_RS07435 0.61 -0.3 1,590,764 - RR42_RS07435 0.61 +0.9 1,590,767 + RR42_RS07435 0.61 +2.8 1,590,767 + RR42_RS07435 0.61 +0.7 1,590,767 + RR42_RS07435 0.61 -1.3 1,590,767 + RR42_RS07435 0.61 -1.0 1,590,767 + RR42_RS07435 0.61 +0.6 1,590,767 + RR42_RS07435 0.61 +1.0 1,590,768 - RR42_RS07435 0.61 +1.8 1,590,768 - RR42_RS07435 0.61 +1.2 1,590,872 + RR42_RS07435 0.69 -0.0 1,590,945 - RR42_RS07435 0.74 -1.6 1,590,945 - RR42_RS07435 0.74 +1.2 1,591,143 + RR42_RS07435 0.88 -2.2 1,591,144 - RR42_RS07435 0.88 +1.3 1,591,145 + RR42_RS07435 0.88 +1.1 1,591,145 + RR42_RS07435 0.88 -0.6 1,591,146 - RR42_RS07435 0.88 +0.6 1,591,146 - RR42_RS07435 0.88 +0.7 1,591,146 - RR42_RS07435 0.88 +0.6 1,591,292 + -1.5 1,591,293 - +0.3 1,591,293 - -1.5 1,591,293 - +0.9 1,591,505 + RR42_RS07440 0.69 +0.4 1,591,519 - RR42_RS07440 0.74 +0.2 1,591,519 - RR42_RS07440 0.74 +1.0 1,592,100 - RR42_RS07445 0.53 -1.1
Or see this region's nucleotide sequence