Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06230 and RR42_RS06235 are separated by 48 nucleotides RR42_RS06235 and RR42_RS06240 are separated by 83 nucleotides RR42_RS06240 and RR42_RS06245 are separated by 14 nucleotides RR42_RS06245 and RR42_RS06250 are separated by 150 nucleotides
RR42_RS06230: RR42_RS06230 - hypothetical protein, at 1,367,726 to 1,368,349
_RS06230
RR42_RS06235: RR42_RS06235 - peptidase M50, at 1,368,398 to 1,369,063
_RS06235
RR42_RS06240: RR42_RS06240 - tryptophan--tRNA ligase, at 1,369,147 to 1,370,349
_RS06240
RR42_RS06245: RR42_RS06245 - SAM-dependent methyltransferase, at 1,370,364 to 1,370,942
_RS06245
RR42_RS06250: RR42_RS06250 - dihydrodipicolinate synthase, at 1,371,093 to 1,371,977
_RS06250
Position (kb)
1369
1370
1371 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1368.384 kb on + strand at 1368.384 kb on + strand at 1368.384 kb on + strand at 1368.385 kb on - strand at 1368.385 kb on - strand at 1368.385 kb on - strand at 1368.482 kb on + strand, within RR42_RS06235 at 1368.553 kb on + strand, within RR42_RS06235 at 1368.593 kb on + strand, within RR42_RS06235 at 1368.932 kb on + strand, within RR42_RS06235 at 1369.061 kb on + strand at 1369.136 kb on + strand at 1369.136 kb on + strand at 1369.137 kb on - strand at 1369.137 kb on - strand at 1369.142 kb on + strand at 1369.142 kb on + strand at 1369.443 kb on + strand, within RR42_RS06240 at 1369.478 kb on - strand, within RR42_RS06240 at 1369.651 kb on + strand, within RR42_RS06240 at 1369.652 kb on - strand, within RR42_RS06240 at 1370.646 kb on + strand, within RR42_RS06245 at 1370.647 kb on - strand, within RR42_RS06245 at 1370.824 kb on - strand, within RR42_RS06245 at 1370.870 kb on + strand, within RR42_RS06245 at 1370.891 kb on + strand at 1370.891 kb on + strand at 1370.892 kb on - strand at 1370.892 kb on - strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.893 kb on + strand at 1370.894 kb on - strand at 1370.894 kb on - strand at 1370.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 1,368,384 + +0.1 1,368,384 + +0.4 1,368,384 + +0.8 1,368,385 - +0.1 1,368,385 - -0.5 1,368,385 - -0.2 1,368,482 + RR42_RS06235 0.13 -0.4 1,368,553 + RR42_RS06235 0.23 -0.5 1,368,593 + RR42_RS06235 0.29 -0.2 1,368,932 + RR42_RS06235 0.80 -0.4 1,369,061 + -0.5 1,369,136 + -1.1 1,369,136 + +1.3 1,369,137 - +1.7 1,369,137 - +0.2 1,369,142 + +0.6 1,369,142 + +0.7 1,369,443 + RR42_RS06240 0.25 +1.7 1,369,478 - RR42_RS06240 0.28 -0.7 1,369,651 + RR42_RS06240 0.42 +0.8 1,369,652 - RR42_RS06240 0.42 +1.6 1,370,646 + RR42_RS06245 0.49 -0.6 1,370,647 - RR42_RS06245 0.49 +0.2 1,370,824 - RR42_RS06245 0.79 -1.0 1,370,870 + RR42_RS06245 0.87 +0.5 1,370,891 + -0.3 1,370,891 + -0.6 1,370,892 - -1.0 1,370,892 - -0.4 1,370,893 + -1.7 1,370,893 + -1.8 1,370,893 + +0.8 1,370,893 + -1.8 1,370,893 + +0.1 1,370,894 - +0.3 1,370,894 - -2.8 1,370,894 - +0.2
Or see this region's nucleotide sequence