Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06225 and RR42_RS06230 are separated by 141 nucleotides RR42_RS06230 and RR42_RS06235 are separated by 48 nucleotides RR42_RS06235 and RR42_RS06240 are separated by 83 nucleotides
RR42_RS06225: RR42_RS06225 - phosphoesterase, at 1,366,739 to 1,367,584
_RS06225
RR42_RS06230: RR42_RS06230 - hypothetical protein, at 1,367,726 to 1,368,349
_RS06230
RR42_RS06235: RR42_RS06235 - peptidase M50, at 1,368,398 to 1,369,063
_RS06235
RR42_RS06240: RR42_RS06240 - tryptophan--tRNA ligase, at 1,369,147 to 1,370,349
_RS06240
Position (kb)
1367
1368
1369 Strain fitness (log2 ratio)
-1
0
1
2 at 1366.727 kb on + strand at 1366.727 kb on + strand at 1366.727 kb on + strand at 1366.728 kb on - strand at 1366.728 kb on - strand at 1366.732 kb on + strand at 1366.928 kb on + strand, within RR42_RS06225 at 1366.928 kb on + strand, within RR42_RS06225 at 1366.928 kb on + strand, within RR42_RS06225 at 1366.928 kb on + strand, within RR42_RS06225 at 1366.929 kb on - strand, within RR42_RS06225 at 1366.978 kb on + strand, within RR42_RS06225 at 1366.979 kb on - strand, within RR42_RS06225 at 1366.979 kb on - strand, within RR42_RS06225 at 1366.979 kb on - strand, within RR42_RS06225 at 1367.222 kb on + strand, within RR42_RS06225 at 1367.246 kb on + strand, within RR42_RS06225 at 1367.337 kb on - strand, within RR42_RS06225 at 1367.342 kb on + strand, within RR42_RS06225 at 1367.426 kb on - strand, within RR42_RS06225 at 1367.447 kb on + strand, within RR42_RS06225 at 1367.448 kb on - strand, within RR42_RS06225 at 1367.468 kb on + strand, within RR42_RS06225 at 1367.469 kb on - strand, within RR42_RS06225 at 1367.582 kb on + strand at 1367.736 kb on - strand at 1368.015 kb on - strand, within RR42_RS06230 at 1368.015 kb on - strand, within RR42_RS06230 at 1368.384 kb on + strand at 1368.384 kb on + strand at 1368.384 kb on + strand at 1368.385 kb on - strand at 1368.385 kb on - strand at 1368.385 kb on - strand at 1368.482 kb on + strand, within RR42_RS06235 at 1368.553 kb on + strand, within RR42_RS06235 at 1368.593 kb on + strand, within RR42_RS06235 at 1368.932 kb on + strand, within RR42_RS06235 at 1369.061 kb on + strand at 1369.136 kb on + strand at 1369.136 kb on + strand at 1369.137 kb on - strand at 1369.137 kb on - strand at 1369.142 kb on + strand at 1369.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 1,366,727 + -0.1 1,366,727 + -0.3 1,366,727 + +0.4 1,366,728 - +0.2 1,366,728 - +0.9 1,366,732 + -0.5 1,366,928 + RR42_RS06225 0.22 +0.5 1,366,928 + RR42_RS06225 0.22 +0.2 1,366,928 + RR42_RS06225 0.22 +1.2 1,366,928 + RR42_RS06225 0.22 +0.8 1,366,929 - RR42_RS06225 0.22 +0.6 1,366,978 + RR42_RS06225 0.28 +1.1 1,366,979 - RR42_RS06225 0.28 +0.7 1,366,979 - RR42_RS06225 0.28 -1.3 1,366,979 - RR42_RS06225 0.28 +1.8 1,367,222 + RR42_RS06225 0.57 +1.2 1,367,246 + RR42_RS06225 0.60 +0.8 1,367,337 - RR42_RS06225 0.71 -0.5 1,367,342 + RR42_RS06225 0.71 +1.7 1,367,426 - RR42_RS06225 0.81 -0.0 1,367,447 + RR42_RS06225 0.84 +1.1 1,367,448 - RR42_RS06225 0.84 -0.2 1,367,468 + RR42_RS06225 0.86 +0.7 1,367,469 - RR42_RS06225 0.86 +0.9 1,367,582 + +0.5 1,367,736 - +0.4 1,368,015 - RR42_RS06230 0.46 +0.4 1,368,015 - RR42_RS06230 0.46 -0.1 1,368,384 + +0.1 1,368,384 + +0.4 1,368,384 + +0.8 1,368,385 - +0.1 1,368,385 - -0.5 1,368,385 - -0.2 1,368,482 + RR42_RS06235 0.13 -0.4 1,368,553 + RR42_RS06235 0.23 -0.5 1,368,593 + RR42_RS06235 0.29 -0.2 1,368,932 + RR42_RS06235 0.80 -0.4 1,369,061 + -0.5 1,369,136 + -1.1 1,369,136 + +1.3 1,369,137 - +1.7 1,369,137 - +0.2 1,369,142 + +0.6 1,369,142 + +0.7
Or see this region's nucleotide sequence