Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05360 and RR42_RS05365 overlap by 16 nucleotides RR42_RS05365 and RR42_RS05370 overlap by 2 nucleotides RR42_RS05370 and RR42_RS05375 are separated by 24 nucleotides RR42_RS05375 and RR42_RS05380 are separated by 38 nucleotides
RR42_RS05360: RR42_RS05360 - membrane protein, at 1,186,341 to 1,186,958
_RS05360
RR42_RS05365: RR42_RS05365 - hypothetical protein, at 1,186,943 to 1,187,275
_RS05365
RR42_RS05370: RR42_RS05370 - ABC transporter permease, at 1,187,274 to 1,189,817
_RS05370
RR42_RS05375: RR42_RS05375 - hemoglobin-like protein, at 1,189,842 to 1,190,279
_RS05375
RR42_RS05380: RR42_RS05380 - membrane protein, at 1,190,318 to 1,190,935
_RS05380
Position (kb)
1187
1188
1189
1190 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1186.280 kb on + strand at 1186.284 kb on - strand at 1186.296 kb on + strand at 1186.345 kb on + strand at 1186.346 kb on - strand at 1186.346 kb on - strand at 1186.346 kb on - strand at 1186.399 kb on - strand at 1186.399 kb on - strand at 1186.399 kb on - strand at 1186.890 kb on + strand, within RR42_RS05360 at 1186.891 kb on - strand, within RR42_RS05360 at 1187.119 kb on + strand, within RR42_RS05365 at 1187.120 kb on - strand, within RR42_RS05365 at 1187.120 kb on - strand, within RR42_RS05365 at 1187.120 kb on - strand, within RR42_RS05365 at 1187.132 kb on + strand, within RR42_RS05365 at 1187.132 kb on + strand, within RR42_RS05365 at 1187.133 kb on - strand, within RR42_RS05365 at 1187.133 kb on - strand, within RR42_RS05365 at 1187.929 kb on - strand, within RR42_RS05370 at 1188.153 kb on + strand, within RR42_RS05370 at 1188.153 kb on + strand, within RR42_RS05370 at 1188.153 kb on + strand, within RR42_RS05370 at 1188.154 kb on - strand, within RR42_RS05370 at 1188.155 kb on + strand, within RR42_RS05370 at 1188.155 kb on + strand, within RR42_RS05370 at 1188.301 kb on - strand, within RR42_RS05370 at 1188.301 kb on - strand, within RR42_RS05370 at 1188.634 kb on + strand, within RR42_RS05370 at 1188.635 kb on - strand, within RR42_RS05370 at 1188.635 kb on - strand, within RR42_RS05370 at 1188.864 kb on - strand, within RR42_RS05370 at 1188.959 kb on + strand, within RR42_RS05370 at 1188.959 kb on + strand, within RR42_RS05370 at 1188.959 kb on + strand, within RR42_RS05370 at 1188.960 kb on - strand, within RR42_RS05370 at 1188.960 kb on - strand, within RR42_RS05370 at 1188.960 kb on - strand, within RR42_RS05370 at 1189.041 kb on + strand, within RR42_RS05370 at 1189.041 kb on + strand, within RR42_RS05370 at 1189.043 kb on + strand, within RR42_RS05370 at 1189.044 kb on - strand, within RR42_RS05370 at 1189.302 kb on + strand, within RR42_RS05370 at 1189.302 kb on + strand, within RR42_RS05370 at 1189.302 kb on + strand, within RR42_RS05370 at 1189.303 kb on - strand, within RR42_RS05370 at 1189.303 kb on - strand, within RR42_RS05370 at 1189.305 kb on - strand, within RR42_RS05370 at 1189.305 kb on - strand, within RR42_RS05370 at 1189.491 kb on + strand, within RR42_RS05370 at 1189.491 kb on + strand, within RR42_RS05370 at 1189.491 kb on + strand, within RR42_RS05370 at 1189.492 kb on - strand, within RR42_RS05370 at 1189.492 kb on - strand, within RR42_RS05370 at 1189.588 kb on - strand at 1189.841 kb on + strand at 1189.841 kb on + strand at 1189.841 kb on + strand at 1189.841 kb on + strand at 1189.842 kb on - strand at 1189.842 kb on - strand at 1189.912 kb on - strand, within RR42_RS05375 at 1189.962 kb on + strand, within RR42_RS05375 at 1189.962 kb on + strand, within RR42_RS05375 at 1189.963 kb on - strand, within RR42_RS05375 at 1189.963 kb on - strand, within RR42_RS05375 at 1190.304 kb on + strand at 1190.305 kb on - strand at 1190.305 kb on - strand at 1190.305 kb on - strand at 1190.305 kb on - strand at 1190.374 kb on - strand at 1190.705 kb on + strand, within RR42_RS05380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 1,186,280 + -0.2 1,186,284 - +0.6 1,186,296 + +0.6 1,186,345 + -0.0 1,186,346 - +0.8 1,186,346 - +1.7 1,186,346 - +1.1 1,186,399 - +0.4 1,186,399 - -0.3 1,186,399 - +1.1 1,186,890 + RR42_RS05360 0.89 +0.5 1,186,891 - RR42_RS05360 0.89 +0.3 1,187,119 + RR42_RS05365 0.53 +0.6 1,187,120 - RR42_RS05365 0.53 +1.3 1,187,120 - RR42_RS05365 0.53 +1.5 1,187,120 - RR42_RS05365 0.53 -0.4 1,187,132 + RR42_RS05365 0.57 +1.6 1,187,132 + RR42_RS05365 0.57 +0.1 1,187,133 - RR42_RS05365 0.57 +0.3 1,187,133 - RR42_RS05365 0.57 -0.0 1,187,929 - RR42_RS05370 0.26 +1.1 1,188,153 + RR42_RS05370 0.35 +0.5 1,188,153 + RR42_RS05370 0.35 +0.2 1,188,153 + RR42_RS05370 0.35 -0.1 1,188,154 - RR42_RS05370 0.35 -0.9 1,188,155 + RR42_RS05370 0.35 -1.6 1,188,155 + RR42_RS05370 0.35 +1.4 1,188,301 - RR42_RS05370 0.40 +0.5 1,188,301 - RR42_RS05370 0.40 -0.5 1,188,634 + RR42_RS05370 0.53 +0.4 1,188,635 - RR42_RS05370 0.53 +0.7 1,188,635 - RR42_RS05370 0.53 -3.1 1,188,864 - RR42_RS05370 0.62 +0.3 1,188,959 + RR42_RS05370 0.66 +0.3 1,188,959 + RR42_RS05370 0.66 -0.4 1,188,959 + RR42_RS05370 0.66 +0.1 1,188,960 - RR42_RS05370 0.66 +1.0 1,188,960 - RR42_RS05370 0.66 -0.7 1,188,960 - RR42_RS05370 0.66 +1.6 1,189,041 + RR42_RS05370 0.69 -0.2 1,189,041 + RR42_RS05370 0.69 +0.6 1,189,043 + RR42_RS05370 0.70 +2.3 1,189,044 - RR42_RS05370 0.70 +0.4 1,189,302 + RR42_RS05370 0.80 +0.7 1,189,302 + RR42_RS05370 0.80 +0.2 1,189,302 + RR42_RS05370 0.80 +0.4 1,189,303 - RR42_RS05370 0.80 +1.5 1,189,303 - RR42_RS05370 0.80 -1.1 1,189,305 - RR42_RS05370 0.80 +1.5 1,189,305 - RR42_RS05370 0.80 -1.8 1,189,491 + RR42_RS05370 0.87 -0.4 1,189,491 + RR42_RS05370 0.87 +0.1 1,189,491 + RR42_RS05370 0.87 -0.1 1,189,492 - RR42_RS05370 0.87 -0.0 1,189,492 - RR42_RS05370 0.87 -0.3 1,189,588 - +0.0 1,189,841 + +0.8 1,189,841 + +0.2 1,189,841 + -0.2 1,189,841 + +0.5 1,189,842 - +0.5 1,189,842 - -0.3 1,189,912 - RR42_RS05375 0.16 -2.3 1,189,962 + RR42_RS05375 0.27 +0.4 1,189,962 + RR42_RS05375 0.27 -1.7 1,189,963 - RR42_RS05375 0.28 +0.1 1,189,963 - RR42_RS05375 0.28 +1.2 1,190,304 + -2.6 1,190,305 - -0.1 1,190,305 - -0.9 1,190,305 - -0.4 1,190,305 - +0.9 1,190,374 - -0.3 1,190,705 + RR42_RS05380 0.63 +0.8
Or see this region's nucleotide sequence