Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03865 and RR42_RS03870 are separated by 112 nucleotides RR42_RS03870 and RR42_RS03875 are separated by 166 nucleotides RR42_RS03875 and RR42_RS03880 are separated by 47 nucleotides RR42_RS03880 and RR42_RS03885 overlap by 1 nucleotides
RR42_RS03865: RR42_RS03865 - hydroxyacid dehydrogenase, at 854,013 to 854,954
_RS03865
RR42_RS03870: RR42_RS03870 - hypothetical protein, at 855,067 to 855,261
_RS03870
RR42_RS03875: RR42_RS03875 - nucleoside 2-deoxyribosyltransferase, at 855,428 to 855,976
_RS03875
RR42_RS03880: RR42_RS03880 - hypothetical protein, at 856,024 to 856,680
_RS03880
RR42_RS03885: RR42_RS03885 - mammalian cell entry protein, at 856,680 to 858,311
_RS03885
Position (kb) 855
856 Strain fitness (log2 ratio) -2
-1
0
1
2 at 854.651 kb on - strand, within RR42_RS03865 at 854.836 kb on + strand, within RR42_RS03865 at 854.836 kb on + strand, within RR42_RS03865 at 854.837 kb on - strand, within RR42_RS03865 at 854.990 kb on + strand at 854.993 kb on - strand at 855.080 kb on - strand at 855.080 kb on - strand at 855.081 kb on + strand at 855.440 kb on + strand at 855.440 kb on + strand at 855.440 kb on + strand at 855.515 kb on + strand, within RR42_RS03875 at 855.516 kb on - strand, within RR42_RS03875 at 856.014 kb on + strand at 856.298 kb on + strand, within RR42_RS03880 at 856.667 kb on - strand at 856.947 kb on + strand, within RR42_RS03885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 854,651 - RR42_RS03865 0.68 -1.3 854,836 + RR42_RS03865 0.87 -0.3 854,836 + RR42_RS03865 0.87 +0.8 854,837 - RR42_RS03865 0.87 +0.3 854,990 + +1.2 854,993 - +0.5 855,080 - -0.1 855,080 - -0.3 855,081 + -0.2 855,440 + -0.1 855,440 + +2.4 855,440 + -1.0 855,515 + RR42_RS03875 0.16 +1.1 855,516 - RR42_RS03875 0.16 +0.5 856,014 + -0.5 856,298 + RR42_RS03880 0.42 -0.2 856,667 - -0.5 856,947 + RR42_RS03885 0.16 -2.0
Or see this region's nucleotide sequence