Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02505 and RR42_RS02510 are separated by 195 nucleotides RR42_RS02510 and RR42_RS02515 are separated by 18 nucleotides
RR42_RS02505: RR42_RS02505 - acyl-CoA dehydrogenase, at 561,906 to 563,693
_RS02505
RR42_RS02510: RR42_RS02510 - 3-hydroxyacyl-CoA dehydrogenase, at 563,889 to 566,315
_RS02510
RR42_RS02515: RR42_RS02515 - acetyl-CoA acetyltransferase, at 566,334 to 567,533
_RS02515
Position (kb) 563
564
565
566
567 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 563.196 kb on + strand, within RR42_RS02505 at 563.196 kb on + strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.508 kb on + strand, within RR42_RS02505 at 563.528 kb on + strand at 563.568 kb on + strand at 563.569 kb on - strand at 563.569 kb on - strand at 563.598 kb on + strand at 563.598 kb on + strand at 563.712 kb on + strand at 563.724 kb on - strand at 563.740 kb on - strand at 563.740 kb on - strand at 563.772 kb on - strand at 563.803 kb on + strand at 563.804 kb on - strand at 563.878 kb on + strand at 563.878 kb on + strand at 564.117 kb on + strand at 564.202 kb on - strand, within RR42_RS02510 at 564.252 kb on - strand, within RR42_RS02510 at 564.345 kb on + strand, within RR42_RS02510 at 564.346 kb on - strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.813 kb on + strand, within RR42_RS02510 at 564.955 kb on - strand, within RR42_RS02510 at 564.955 kb on - strand, within RR42_RS02510 at 564.966 kb on + strand, within RR42_RS02510 at 565.248 kb on + strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.738 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.359 kb on - strand at 566.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 563,196 + RR42_RS02505 0.72 -2.6 563,196 + RR42_RS02505 0.72 -1.7 563,197 - RR42_RS02505 0.72 -1.1 563,197 - RR42_RS02505 0.72 -2.1 563,226 + RR42_RS02505 0.74 -1.1 563,226 + RR42_RS02505 0.74 -2.6 563,227 - RR42_RS02505 0.74 -1.0 563,227 - RR42_RS02505 0.74 -1.3 563,475 + RR42_RS02505 0.88 -3.9 563,475 + RR42_RS02505 0.88 +1.8 563,476 - RR42_RS02505 0.88 -2.6 563,476 - RR42_RS02505 0.88 +0.4 563,476 - RR42_RS02505 0.88 -1.9 563,508 + RR42_RS02505 0.90 -1.0 563,528 + -1.6 563,568 + -2.1 563,569 - -1.3 563,569 - -1.9 563,598 + -1.8 563,598 + -0.4 563,712 + -1.6 563,724 - +0.1 563,740 - -0.6 563,740 - +0.7 563,772 - +1.1 563,803 + -0.2 563,804 - -1.0 563,878 + +1.9 563,878 + +1.4 564,117 + +1.2 564,202 - RR42_RS02510 0.13 -0.5 564,252 - RR42_RS02510 0.15 -1.1 564,345 + RR42_RS02510 0.19 +1.2 564,346 - RR42_RS02510 0.19 +0.3 564,528 + RR42_RS02510 0.26 +2.7 564,528 + RR42_RS02510 0.26 +0.5 564,690 + RR42_RS02510 0.33 -0.6 564,690 + RR42_RS02510 0.33 -0.2 564,690 + RR42_RS02510 0.33 -0.2 564,762 + RR42_RS02510 0.36 -2.1 564,762 + RR42_RS02510 0.36 -0.3 564,762 + RR42_RS02510 0.36 +0.3 564,813 + RR42_RS02510 0.38 -1.4 564,955 - RR42_RS02510 0.44 -0.5 564,955 - RR42_RS02510 0.44 +1.0 564,966 + RR42_RS02510 0.44 +0.4 565,248 + RR42_RS02510 0.56 +0.3 565,340 - RR42_RS02510 0.60 +0.5 565,340 - RR42_RS02510 0.60 +0.3 565,635 + RR42_RS02510 0.72 -1.4 565,635 + RR42_RS02510 0.72 +0.5 565,737 + RR42_RS02510 0.76 +0.5 565,737 + RR42_RS02510 0.76 -0.1 565,737 + RR42_RS02510 0.76 -0.1 565,737 + RR42_RS02510 0.76 +1.1 565,738 - RR42_RS02510 0.76 +0.6 565,846 - RR42_RS02510 0.81 -0.8 565,846 - RR42_RS02510 0.81 -0.6 566,358 + +0.0 566,358 + +0.9 566,358 + -0.3 566,358 + -2.9 566,358 + +0.7 566,359 - +0.0 566,359 - -0.5
Or see this region's nucleotide sequence