Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00710 and RR42_RS00715 are separated by 896 nucleotides RR42_RS00715 and RR42_RS00720 are separated by 219 nucleotides
RR42_RS00710: RR42_RS00710 - ADP-heptose synthase, at 179,359 to 179,847
_RS00710
RR42_RS00715: RR42_RS00715 - ferritin, at 180,744 to 181,607
_RS00715
RR42_RS00720: RR42_RS00720 - patatin, at 181,827 to 183,068
_RS00720
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 179.937 kb on + strand at 179.937 kb on + strand at 179.937 kb on + strand at 179.937 kb on + strand at 179.938 kb on - strand at 180.371 kb on - strand at 180.371 kb on - strand at 180.371 kb on - strand at 180.371 kb on - strand at 180.603 kb on + strand at 180.604 kb on - strand at 180.604 kb on - strand at 180.613 kb on + strand at 180.614 kb on - strand at 180.745 kb on + strand at 180.745 kb on + strand at 180.745 kb on + strand at 180.745 kb on + strand at 180.746 kb on - strand at 180.746 kb on - strand at 180.858 kb on - strand, within RR42_RS00715 at 180.858 kb on - strand, within RR42_RS00715 at 181.103 kb on - strand, within RR42_RS00715 at 181.166 kb on - strand, within RR42_RS00715 at 181.611 kb on - strand at 181.721 kb on - strand at 181.773 kb on - strand at 181.773 kb on - strand at 181.773 kb on - strand at 181.773 kb on - strand at 181.824 kb on - strand at 181.866 kb on - strand at 181.866 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 182.018 kb on - strand, within RR42_RS00720 at 182.018 kb on - strand, within RR42_RS00720 at 182.584 kb on + strand, within RR42_RS00720 at 182.585 kb on - strand, within RR42_RS00720 at 182.585 kb on - strand, within RR42_RS00720
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 179,937 + +0.2 179,937 + +1.0 179,937 + -0.4 179,937 + +0.9 179,938 - +0.3 180,371 - +0.3 180,371 - +0.4 180,371 - +0.2 180,371 - -2.1 180,603 + +0.9 180,604 - -1.1 180,604 - -0.5 180,613 + -1.6 180,614 - +0.5 180,745 + -3.7 180,745 + -0.6 180,745 + -1.7 180,745 + -1.2 180,746 - -1.6 180,746 - -2.6 180,858 - RR42_RS00715 0.13 -0.9 180,858 - RR42_RS00715 0.13 -2.6 181,103 - RR42_RS00715 0.42 -1.2 181,166 - RR42_RS00715 0.49 -2.0 181,611 - +0.8 181,721 - +1.8 181,773 - +1.7 181,773 - +2.1 181,773 - -1.0 181,773 - +1.1 181,824 - +2.5 181,866 - +2.0 181,866 - +0.3 181,925 - +3.9 181,925 - +1.8 181,925 - +1.8 181,925 - +2.3 181,925 - +2.1 181,925 - +2.3 181,925 - +1.4 182,018 - RR42_RS00720 0.15 +2.0 182,018 - RR42_RS00720 0.15 +1.1 182,584 + RR42_RS00720 0.61 -3.2 182,585 - RR42_RS00720 0.61 +1.0 182,585 - RR42_RS00720 0.61 +0.2
Or see this region's nucleotide sequence