Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35655 and RR42_RS35660 are separated by 927 nucleotides RR42_RS35660 and RR42_RS35665 overlap by 2 nucleotides RR42_RS35665 and RR42_RS35670 are separated by 21 nucleotides
RR42_RS35655: RR42_RS35655 - hypothetical protein, at 3,451,325 to 3,451,780
_RS35655
RR42_RS35660: RR42_RS35660 - hypothetical protein, at 3,452,708 to 3,453,019
_RS35660
RR42_RS35665: RR42_RS35665 - hypothetical protein, at 3,453,018 to 3,453,284
_RS35665
RR42_RS35670: RR42_RS35670 - cytochrome B oxidoreductase, at 3,453,306 to 3,453,857
_RS35670
Position (kb)
3452
3453
3454 Strain fitness (log2 ratio)
-2
-1
0
1 at 3451.788 kb on - strand at 3451.788 kb on - strand at 3451.788 kb on - strand at 3451.791 kb on - strand at 3451.791 kb on - strand at 3451.835 kb on - strand at 3452.030 kb on + strand at 3452.030 kb on + strand at 3452.030 kb on + strand at 3452.092 kb on - strand at 3452.209 kb on + strand at 3452.209 kb on + strand at 3452.370 kb on + strand at 3452.371 kb on - strand at 3452.431 kb on + strand at 3452.431 kb on + strand at 3452.445 kb on - strand at 3452.445 kb on - strand at 3452.584 kb on + strand at 3452.584 kb on + strand at 3452.601 kb on + strand at 3452.601 kb on + strand at 3452.601 kb on + strand at 3452.601 kb on + strand at 3452.602 kb on - strand at 3452.602 kb on - strand at 3452.602 kb on - strand at 3452.602 kb on - strand at 3452.718 kb on + strand at 3452.719 kb on - strand at 3452.719 kb on - strand at 3452.733 kb on - strand at 3452.733 kb on - strand at 3452.804 kb on + strand, within RR42_RS35660 at 3452.807 kb on + strand, within RR42_RS35660 at 3452.808 kb on - strand, within RR42_RS35660 at 3452.966 kb on - strand, within RR42_RS35660 at 3452.983 kb on + strand, within RR42_RS35660 at 3452.983 kb on + strand, within RR42_RS35660 at 3452.984 kb on - strand, within RR42_RS35660 at 3453.153 kb on + strand, within RR42_RS35665 at 3453.175 kb on + strand, within RR42_RS35665 at 3453.175 kb on + strand, within RR42_RS35665 at 3453.175 kb on + strand, within RR42_RS35665 at 3453.175 kb on + strand, within RR42_RS35665 at 3453.190 kb on - strand, within RR42_RS35665 at 3453.198 kb on + strand, within RR42_RS35665 at 3453.199 kb on - strand, within RR42_RS35665 at 3453.234 kb on + strand, within RR42_RS35665 at 3453.237 kb on + strand, within RR42_RS35665 at 3453.237 kb on + strand, within RR42_RS35665 at 3453.238 kb on - strand, within RR42_RS35665 at 3453.282 kb on + strand at 3453.282 kb on + strand at 3453.436 kb on - strand, within RR42_RS35670 at 3453.580 kb on + strand, within RR42_RS35670 at 3453.580 kb on + strand, within RR42_RS35670 at 3453.581 kb on - strand, within RR42_RS35670 at 3453.581 kb on - strand, within RR42_RS35670 at 3453.587 kb on + strand, within RR42_RS35670 at 3453.587 kb on + strand, within RR42_RS35670 at 3453.587 kb on + strand, within RR42_RS35670 at 3453.668 kb on - strand, within RR42_RS35670 at 3453.734 kb on - strand, within RR42_RS35670 at 3453.740 kb on - strand, within RR42_RS35670 at 3453.876 kb on + strand at 3453.876 kb on + strand at 3453.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 3,451,788 - -0.5 3,451,788 - +0.0 3,451,788 - +0.3 3,451,791 - +0.3 3,451,791 - -1.1 3,451,835 - +1.0 3,452,030 + +0.1 3,452,030 + -0.0 3,452,030 + -0.5 3,452,092 - -0.4 3,452,209 + +0.2 3,452,209 + -0.6 3,452,370 + -0.1 3,452,371 - -0.0 3,452,431 + -0.9 3,452,431 + -0.1 3,452,445 - +0.4 3,452,445 - +1.6 3,452,584 + -0.8 3,452,584 + -0.3 3,452,601 + -0.1 3,452,601 + +0.5 3,452,601 + +0.4 3,452,601 + +0.0 3,452,602 - -0.0 3,452,602 - +0.3 3,452,602 - -0.1 3,452,602 - +0.5 3,452,718 + -0.1 3,452,719 - +0.0 3,452,719 - -0.2 3,452,733 - -0.4 3,452,733 - +0.7 3,452,804 + RR42_RS35660 0.31 -0.0 3,452,807 + RR42_RS35660 0.32 -0.1 3,452,808 - RR42_RS35660 0.32 -0.2 3,452,966 - RR42_RS35660 0.83 -0.3 3,452,983 + RR42_RS35660 0.88 -0.1 3,452,983 + RR42_RS35660 0.88 -0.5 3,452,984 - RR42_RS35660 0.88 -0.4 3,453,153 + RR42_RS35665 0.51 -0.0 3,453,175 + RR42_RS35665 0.59 +0.2 3,453,175 + RR42_RS35665 0.59 -0.6 3,453,175 + RR42_RS35665 0.59 -0.2 3,453,175 + RR42_RS35665 0.59 +0.8 3,453,190 - RR42_RS35665 0.64 -0.5 3,453,198 + RR42_RS35665 0.67 -0.4 3,453,199 - RR42_RS35665 0.68 +0.3 3,453,234 + RR42_RS35665 0.81 +0.6 3,453,237 + RR42_RS35665 0.82 -0.0 3,453,237 + RR42_RS35665 0.82 +0.1 3,453,238 - RR42_RS35665 0.82 -0.1 3,453,282 + -2.2 3,453,282 + +0.7 3,453,436 - RR42_RS35670 0.24 +0.4 3,453,580 + RR42_RS35670 0.50 -0.2 3,453,580 + RR42_RS35670 0.50 +0.6 3,453,581 - RR42_RS35670 0.50 +0.7 3,453,581 - RR42_RS35670 0.50 -2.1 3,453,587 + RR42_RS35670 0.51 -1.1 3,453,587 + RR42_RS35670 0.51 +1.1 3,453,587 + RR42_RS35670 0.51 -1.6 3,453,668 - RR42_RS35670 0.66 +1.1 3,453,734 - RR42_RS35670 0.78 -0.7 3,453,740 - RR42_RS35670 0.79 +0.2 3,453,876 + +0.2 3,453,876 + +0.3 3,453,877 - +0.3
Or see this region's nucleotide sequence