Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31925 and RR42_RS31930 are separated by 54 nucleotides RR42_RS31930 and RR42_RS31935 are separated by 9 nucleotides RR42_RS31935 and RR42_RS31940 are separated by 45 nucleotides
RR42_RS31925: RR42_RS31925 - acyl-CoA dehydrogenase, at 2,539,681 to 2,540,751
_RS31925
RR42_RS31930: RR42_RS31930 - acyl-CoA dehydrogenase, at 2,540,806 to 2,541,975
_RS31930
RR42_RS31935: RR42_RS31935 - enoyl-CoA hydratase, at 2,541,985 to 2,542,770
_RS31935
RR42_RS31940: RR42_RS31940 - 2-nitropropane dioxygenase, at 2,542,816 to 2,543,925
_RS31940
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-1
0
1 at 2540.874 kb on - strand at 2541.051 kb on + strand, within RR42_RS31930 at 2541.086 kb on + strand, within RR42_RS31930 at 2541.527 kb on + strand, within RR42_RS31930 at 2541.528 kb on - strand, within RR42_RS31930 at 2541.740 kb on + strand, within RR42_RS31930 at 2541.741 kb on - strand, within RR42_RS31930 at 2541.741 kb on - strand, within RR42_RS31930 at 2541.962 kb on + strand at 2542.074 kb on + strand, within RR42_RS31935 at 2542.075 kb on - strand, within RR42_RS31935 at 2542.077 kb on - strand, within RR42_RS31935 at 2542.110 kb on - strand, within RR42_RS31935 at 2542.302 kb on - strand, within RR42_RS31935 at 2542.302 kb on - strand, within RR42_RS31935 at 2542.416 kb on + strand, within RR42_RS31935 at 2542.517 kb on + strand, within RR42_RS31935 at 2542.518 kb on - strand, within RR42_RS31935 at 2542.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,540,874 - -0.1 2,541,051 + RR42_RS31930 0.21 -0.9 2,541,086 + RR42_RS31930 0.24 -0.4 2,541,527 + RR42_RS31930 0.62 +0.8 2,541,528 - RR42_RS31930 0.62 +0.6 2,541,740 + RR42_RS31930 0.80 +0.8 2,541,741 - RR42_RS31930 0.80 +1.0 2,541,741 - RR42_RS31930 0.80 +0.9 2,541,962 + -1.0 2,542,074 + RR42_RS31935 0.11 +0.3 2,542,075 - RR42_RS31935 0.11 -0.5 2,542,077 - RR42_RS31935 0.12 -0.3 2,542,110 - RR42_RS31935 0.16 +1.1 2,542,302 - RR42_RS31935 0.40 +1.1 2,542,302 - RR42_RS31935 0.40 -0.9 2,542,416 + RR42_RS31935 0.55 +1.0 2,542,517 + RR42_RS31935 0.68 -0.0 2,542,518 - RR42_RS31935 0.68 +1.2 2,542,775 - -1.4
Or see this region's nucleotide sequence