Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30610 and RR42_RS30615 are separated by 57 nucleotides RR42_RS30615 and RR42_RS30620 overlap by 4 nucleotides RR42_RS30620 and RR42_RS30625 are separated by 133 nucleotides RR42_RS30625 and RR42_RS30630 are separated by 16 nucleotides RR42_RS30630 and RR42_RS30635 are separated by 172 nucleotides
RR42_RS30610: RR42_RS30610 - cystathionine beta-lyase, at 2,245,319 to 2,246,536
_RS30610
RR42_RS30615: RR42_RS30615 - plasmid stabilization protein, at 2,246,594 to 2,246,839
_RS30615
RR42_RS30620: RR42_RS30620 - plasmid stabilization protein, at 2,246,836 to 2,247,255
_RS30620
RR42_RS30625: RR42_RS30625 - cob(II)yrinic acid a,c-diamide reductase, at 2,247,389 to 2,248,054
_RS30625
RR42_RS30630: RR42_RS30630 - AsnC family transcriptional regulator, at 2,248,071 to 2,248,541
_RS30630
RR42_RS30635: RR42_RS30635 - hypothetical protein, at 2,248,714 to 2,249,034
_RS30635
Position (kb)
2247
2248
2249 Strain fitness (log2 ratio)
-2
-1
0
1 at 2246.554 kb on + strand at 2246.555 kb on - strand at 2246.555 kb on - strand at 2246.582 kb on + strand at 2246.583 kb on - strand at 2246.843 kb on + strand at 2246.843 kb on + strand at 2246.843 kb on + strand at 2246.850 kb on + strand at 2246.851 kb on - strand at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.900 kb on + strand, within RR42_RS30620 at 2246.901 kb on - strand, within RR42_RS30620 at 2246.932 kb on + strand, within RR42_RS30620 at 2246.933 kb on - strand, within RR42_RS30620 at 2246.966 kb on + strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.967 kb on - strand, within RR42_RS30620 at 2246.968 kb on + strand, within RR42_RS30620 at 2246.968 kb on + strand, within RR42_RS30620 at 2247.059 kb on + strand, within RR42_RS30620 at 2247.059 kb on + strand, within RR42_RS30620 at 2247.059 kb on + strand, within RR42_RS30620 at 2247.060 kb on - strand, within RR42_RS30620 at 2247.060 kb on - strand, within RR42_RS30620 at 2247.060 kb on - strand, within RR42_RS30620 at 2247.079 kb on - strand, within RR42_RS30620 at 2247.079 kb on - strand, within RR42_RS30620 at 2247.091 kb on + strand, within RR42_RS30620 at 2247.092 kb on - strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.162 kb on + strand, within RR42_RS30620 at 2247.163 kb on - strand, within RR42_RS30620 at 2247.163 kb on - strand, within RR42_RS30620 at 2247.163 kb on - strand, within RR42_RS30620 at 2247.222 kb on - strand at 2247.222 kb on - strand at 2247.287 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.345 kb on + strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.346 kb on - strand at 2247.440 kb on + strand at 2247.440 kb on + strand at 2247.441 kb on - strand at 2247.441 kb on - strand at 2247.441 kb on - strand at 2247.574 kb on + strand, within RR42_RS30625 at 2247.923 kb on - strand, within RR42_RS30625 at 2247.959 kb on + strand, within RR42_RS30625 at 2247.959 kb on + strand, within RR42_RS30625 at 2248.151 kb on - strand, within RR42_RS30630 at 2248.151 kb on - strand, within RR42_RS30630 at 2248.151 kb on - strand, within RR42_RS30630 at 2248.151 kb on - strand, within RR42_RS30630 at 2248.213 kb on + strand, within RR42_RS30630 at 2248.213 kb on + strand, within RR42_RS30630 at 2248.213 kb on + strand, within RR42_RS30630 at 2248.214 kb on - strand, within RR42_RS30630 at 2248.214 kb on - strand, within RR42_RS30630 at 2248.214 kb on - strand, within RR42_RS30630 at 2248.219 kb on + strand, within RR42_RS30630 at 2248.219 kb on + strand, within RR42_RS30630 at 2248.219 kb on + strand, within RR42_RS30630 at 2248.220 kb on - strand, within RR42_RS30630 at 2248.237 kb on + strand, within RR42_RS30630 at 2248.237 kb on + strand, within RR42_RS30630 at 2248.376 kb on - strand, within RR42_RS30630 at 2248.575 kb on + strand at 2248.589 kb on - strand at 2248.594 kb on + strand at 2248.595 kb on - strand at 2248.658 kb on + strand at 2249.032 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,246,554 + -0.2 2,246,555 - -0.4 2,246,555 - +0.0 2,246,582 + -0.1 2,246,583 - -0.3 2,246,843 + -0.1 2,246,843 + -0.1 2,246,843 + +0.4 2,246,850 + -0.7 2,246,851 - -0.1 2,246,900 + RR42_RS30620 0.15 +1.1 2,246,900 + RR42_RS30620 0.15 -0.3 2,246,900 + RR42_RS30620 0.15 -0.7 2,246,900 + RR42_RS30620 0.15 +0.2 2,246,900 + RR42_RS30620 0.15 -0.8 2,246,900 + RR42_RS30620 0.15 -0.1 2,246,901 - RR42_RS30620 0.15 -0.0 2,246,932 + RR42_RS30620 0.23 -0.2 2,246,933 - RR42_RS30620 0.23 +0.9 2,246,966 + RR42_RS30620 0.31 -0.2 2,246,967 - RR42_RS30620 0.31 -0.5 2,246,967 - RR42_RS30620 0.31 -0.7 2,246,967 - RR42_RS30620 0.31 -0.7 2,246,967 - RR42_RS30620 0.31 +1.1 2,246,968 + RR42_RS30620 0.31 -0.0 2,246,968 + RR42_RS30620 0.31 +0.3 2,247,059 + RR42_RS30620 0.53 +0.3 2,247,059 + RR42_RS30620 0.53 +1.3 2,247,059 + RR42_RS30620 0.53 +0.6 2,247,060 - RR42_RS30620 0.53 +0.4 2,247,060 - RR42_RS30620 0.53 +0.9 2,247,060 - RR42_RS30620 0.53 -0.5 2,247,079 - RR42_RS30620 0.58 +1.3 2,247,079 - RR42_RS30620 0.58 -1.2 2,247,091 + RR42_RS30620 0.61 +0.2 2,247,092 - RR42_RS30620 0.61 -2.4 2,247,162 + RR42_RS30620 0.78 -0.5 2,247,162 + RR42_RS30620 0.78 -0.6 2,247,162 + RR42_RS30620 0.78 +0.3 2,247,162 + RR42_RS30620 0.78 -0.2 2,247,163 - RR42_RS30620 0.78 +1.3 2,247,163 - RR42_RS30620 0.78 +0.3 2,247,163 - RR42_RS30620 0.78 +0.2 2,247,222 - +0.3 2,247,222 - -0.7 2,247,287 + -0.4 2,247,345 + -0.2 2,247,345 + +0.4 2,247,345 + +0.3 2,247,345 + +0.9 2,247,345 + -0.9 2,247,345 + -2.1 2,247,345 + -1.6 2,247,345 + -0.7 2,247,346 - +0.4 2,247,346 - -0.4 2,247,346 - +0.1 2,247,346 - -0.3 2,247,346 - +0.0 2,247,346 - +0.1 2,247,346 - -0.1 2,247,346 - +0.6 2,247,440 + +0.7 2,247,440 + -0.6 2,247,441 - +0.2 2,247,441 - -0.5 2,247,441 - +0.0 2,247,574 + RR42_RS30625 0.28 -1.0 2,247,923 - RR42_RS30625 0.80 -0.2 2,247,959 + RR42_RS30625 0.86 -0.4 2,247,959 + RR42_RS30625 0.86 -1.6 2,248,151 - RR42_RS30630 0.17 -0.4 2,248,151 - RR42_RS30630 0.17 +1.3 2,248,151 - RR42_RS30630 0.17 +0.7 2,248,151 - RR42_RS30630 0.17 -0.2 2,248,213 + RR42_RS30630 0.30 -0.1 2,248,213 + RR42_RS30630 0.30 -0.0 2,248,213 + RR42_RS30630 0.30 -0.4 2,248,214 - RR42_RS30630 0.30 -0.2 2,248,214 - RR42_RS30630 0.30 -0.6 2,248,214 - RR42_RS30630 0.30 -0.1 2,248,219 + RR42_RS30630 0.31 -0.6 2,248,219 + RR42_RS30630 0.31 +0.2 2,248,219 + RR42_RS30630 0.31 -0.1 2,248,220 - RR42_RS30630 0.32 -0.3 2,248,237 + RR42_RS30630 0.35 -0.1 2,248,237 + RR42_RS30630 0.35 -0.5 2,248,376 - RR42_RS30630 0.65 -0.2 2,248,575 + +0.2 2,248,589 - +0.1 2,248,594 + +0.6 2,248,595 - -1.5 2,248,658 + +0.6 2,249,032 + +0.5
Or see this region's nucleotide sequence