Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15710 and RR42_RS15715 overlap by 4 nucleotides RR42_RS15715 and RR42_RS15720 are separated by 133 nucleotides
RR42_RS15710: RR42_RS15710 - LPS biosynthesis protein, at 3,394,992 to 3,396,242
_RS15710
RR42_RS15715: RR42_RS15715 - glycosyl transferase, at 3,396,239 to 3,397,315
_RS15715
RR42_RS15720: RR42_RS15720 - hypothetical protein, at 3,397,449 to 3,398,375
_RS15720
Position (kb)
3396
3397
3398 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3395.278 kb on - strand, within RR42_RS15710 at 3395.426 kb on + strand, within RR42_RS15710 at 3395.427 kb on - strand, within RR42_RS15710 at 3395.569 kb on - strand, within RR42_RS15710 at 3395.582 kb on - strand, within RR42_RS15710 at 3395.766 kb on + strand, within RR42_RS15710 at 3395.766 kb on + strand, within RR42_RS15710 at 3395.811 kb on + strand, within RR42_RS15710 at 3395.811 kb on + strand, within RR42_RS15710 at 3395.812 kb on - strand, within RR42_RS15710 at 3395.891 kb on - strand, within RR42_RS15710 at 3395.943 kb on + strand, within RR42_RS15710 at 3396.157 kb on - strand at 3396.158 kb on + strand at 3396.159 kb on - strand at 3396.253 kb on + strand at 3396.253 kb on + strand at 3396.254 kb on - strand at 3396.254 kb on - strand at 3396.254 kb on - strand at 3396.452 kb on + strand, within RR42_RS15715 at 3396.452 kb on + strand, within RR42_RS15715 at 3396.453 kb on - strand, within RR42_RS15715 at 3396.843 kb on + strand, within RR42_RS15715 at 3396.843 kb on + strand, within RR42_RS15715 at 3396.844 kb on - strand, within RR42_RS15715 at 3396.844 kb on - strand, within RR42_RS15715 at 3396.997 kb on - strand, within RR42_RS15715 at 3397.032 kb on + strand, within RR42_RS15715 at 3397.032 kb on + strand, within RR42_RS15715 at 3397.033 kb on - strand, within RR42_RS15715 at 3397.060 kb on + strand, within RR42_RS15715 at 3397.061 kb on - strand, within RR42_RS15715 at 3397.098 kb on + strand, within RR42_RS15715 at 3397.099 kb on - strand, within RR42_RS15715 at 3397.162 kb on - strand, within RR42_RS15715 at 3397.162 kb on - strand, within RR42_RS15715 at 3397.236 kb on + strand at 3397.237 kb on - strand at 3397.237 kb on - strand at 3397.237 kb on - strand at 3397.416 kb on + strand at 3397.417 kb on - strand at 3397.453 kb on + strand at 3397.453 kb on + strand at 3397.454 kb on - strand at 3397.462 kb on + strand at 3397.465 kb on - strand at 3397.468 kb on + strand at 3397.468 kb on + strand at 3397.468 kb on + strand at 3397.469 kb on - strand at 3397.469 kb on - strand at 3397.469 kb on - strand at 3397.471 kb on + strand at 3397.542 kb on + strand, within RR42_RS15720 at 3397.542 kb on + strand, within RR42_RS15720 at 3397.542 kb on + strand, within RR42_RS15720 at 3397.543 kb on - strand, within RR42_RS15720 at 3397.714 kb on + strand, within RR42_RS15720 at 3397.784 kb on + strand, within RR42_RS15720 at 3397.795 kb on + strand, within RR42_RS15720 at 3397.795 kb on + strand, within RR42_RS15720 at 3397.800 kb on - strand, within RR42_RS15720 at 3397.800 kb on - strand, within RR42_RS15720 at 3397.823 kb on + strand, within RR42_RS15720 at 3397.982 kb on + strand, within RR42_RS15720 at 3397.982 kb on + strand, within RR42_RS15720 at 3398.064 kb on + strand, within RR42_RS15720 at 3398.077 kb on - strand, within RR42_RS15720 at 3398.152 kb on + strand, within RR42_RS15720 at 3398.192 kb on + strand, within RR42_RS15720 at 3398.260 kb on - strand, within RR42_RS15720 at 3398.298 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 3,395,278 - RR42_RS15710 0.23 -2.9 3,395,426 + RR42_RS15710 0.35 -2.2 3,395,427 - RR42_RS15710 0.35 -1.6 3,395,569 - RR42_RS15710 0.46 -1.1 3,395,582 - RR42_RS15710 0.47 -1.1 3,395,766 + RR42_RS15710 0.62 -1.9 3,395,766 + RR42_RS15710 0.62 -0.8 3,395,811 + RR42_RS15710 0.65 +0.4 3,395,811 + RR42_RS15710 0.65 -1.5 3,395,812 - RR42_RS15710 0.66 -2.4 3,395,891 - RR42_RS15710 0.72 -2.1 3,395,943 + RR42_RS15710 0.76 -2.2 3,396,157 - -1.3 3,396,158 + +0.3 3,396,159 - -2.5 3,396,253 + +0.0 3,396,253 + +0.3 3,396,254 - -1.1 3,396,254 - -0.6 3,396,254 - -1.8 3,396,452 + RR42_RS15715 0.20 -0.8 3,396,452 + RR42_RS15715 0.20 -0.6 3,396,453 - RR42_RS15715 0.20 -0.4 3,396,843 + RR42_RS15715 0.56 -0.8 3,396,843 + RR42_RS15715 0.56 -0.3 3,396,844 - RR42_RS15715 0.56 -0.5 3,396,844 - RR42_RS15715 0.56 -1.4 3,396,997 - RR42_RS15715 0.70 -0.1 3,397,032 + RR42_RS15715 0.74 -0.1 3,397,032 + RR42_RS15715 0.74 -1.3 3,397,033 - RR42_RS15715 0.74 +0.6 3,397,060 + RR42_RS15715 0.76 -0.4 3,397,061 - RR42_RS15715 0.76 +1.0 3,397,098 + RR42_RS15715 0.80 -0.5 3,397,099 - RR42_RS15715 0.80 -0.4 3,397,162 - RR42_RS15715 0.86 -1.6 3,397,162 - RR42_RS15715 0.86 -0.7 3,397,236 + +0.1 3,397,237 - -0.3 3,397,237 - +1.3 3,397,237 - +0.0 3,397,416 + +0.0 3,397,417 - -1.8 3,397,453 + -0.2 3,397,453 + +0.6 3,397,454 - -0.3 3,397,462 + +1.1 3,397,465 - -0.9 3,397,468 + -0.2 3,397,468 + -0.2 3,397,468 + -0.6 3,397,469 - -1.0 3,397,469 - -1.2 3,397,469 - +0.2 3,397,471 + -0.2 3,397,542 + RR42_RS15720 0.10 +0.2 3,397,542 + RR42_RS15720 0.10 -0.3 3,397,542 + RR42_RS15720 0.10 -0.3 3,397,543 - RR42_RS15720 0.10 +0.0 3,397,714 + RR42_RS15720 0.29 -0.4 3,397,784 + RR42_RS15720 0.36 -1.8 3,397,795 + RR42_RS15720 0.37 -1.7 3,397,795 + RR42_RS15720 0.37 -0.8 3,397,800 - RR42_RS15720 0.38 +0.5 3,397,800 - RR42_RS15720 0.38 +0.9 3,397,823 + RR42_RS15720 0.40 -1.7 3,397,982 + RR42_RS15720 0.57 +0.1 3,397,982 + RR42_RS15720 0.57 -0.6 3,398,064 + RR42_RS15720 0.66 -2.4 3,398,077 - RR42_RS15720 0.68 -0.8 3,398,152 + RR42_RS15720 0.76 -1.5 3,398,192 + RR42_RS15720 0.80 -0.5 3,398,260 - RR42_RS15720 0.87 -1.3 3,398,298 + +0.2
Or see this region's nucleotide sequence