Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15590 and RR42_RS15595 are separated by 82 nucleotides RR42_RS15595 and RR42_RS15600 are separated by 76 nucleotides
RR42_RS15590: RR42_RS15590 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,366,920 to 3,368,449
_RS15590
RR42_RS15595: RR42_RS15595 - acetoacetyl-CoA synthetase, at 3,368,532 to 3,370,568
_RS15595
RR42_RS15600: RR42_RS15600 - glutathione S-transferase, at 3,370,645 to 3,371,301
_RS15600
Position (kb)
3368
3369
3370
3371 Strain fitness (log2 ratio)
-1
0
1
2 at 3368.464 kb on + strand at 3368.464 kb on + strand at 3368.465 kb on - strand at 3368.715 kb on + strand at 3368.808 kb on + strand, within RR42_RS15595 at 3368.808 kb on + strand, within RR42_RS15595 at 3368.809 kb on - strand, within RR42_RS15595 at 3368.809 kb on - strand, within RR42_RS15595 at 3368.809 kb on - strand, within RR42_RS15595 at 3368.992 kb on - strand, within RR42_RS15595 at 3369.473 kb on + strand, within RR42_RS15595 at 3369.504 kb on + strand, within RR42_RS15595 at 3369.504 kb on + strand, within RR42_RS15595 at 3369.505 kb on - strand, within RR42_RS15595 at 3369.577 kb on - strand, within RR42_RS15595 at 3369.719 kb on - strand, within RR42_RS15595 at 3369.771 kb on + strand, within RR42_RS15595 at 3369.771 kb on + strand, within RR42_RS15595 at 3369.772 kb on - strand, within RR42_RS15595 at 3369.879 kb on + strand, within RR42_RS15595 at 3370.122 kb on + strand, within RR42_RS15595 at 3370.123 kb on - strand, within RR42_RS15595 at 3370.240 kb on - strand, within RR42_RS15595 at 3370.240 kb on - strand, within RR42_RS15595 at 3370.240 kb on - strand, within RR42_RS15595 at 3370.257 kb on + strand, within RR42_RS15595 at 3370.257 kb on + strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.258 kb on - strand, within RR42_RS15595 at 3370.259 kb on + strand, within RR42_RS15595 at 3370.260 kb on - strand, within RR42_RS15595 at 3370.260 kb on - strand, within RR42_RS15595 at 3370.509 kb on + strand at 3370.510 kb on - strand at 3370.742 kb on + strand, within RR42_RS15600 at 3370.775 kb on + strand, within RR42_RS15600 at 3370.775 kb on + strand, within RR42_RS15600 at 3370.776 kb on - strand, within RR42_RS15600 at 3370.776 kb on - strand, within RR42_RS15600 at 3370.806 kb on - strand, within RR42_RS15600 at 3370.806 kb on - strand, within RR42_RS15600 at 3370.845 kb on - strand, within RR42_RS15600 at 3371.079 kb on - strand, within RR42_RS15600 at 3371.272 kb on - strand at 3371.435 kb on + strand at 3371.436 kb on - strand at 3371.449 kb on - strand at 3371.451 kb on + strand at 3371.451 kb on + strand at 3371.451 kb on + strand at 3371.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 3,368,464 + -0.5 3,368,464 + -0.5 3,368,465 - +0.3 3,368,715 + +0.1 3,368,808 + RR42_RS15595 0.14 -0.4 3,368,808 + RR42_RS15595 0.14 -0.8 3,368,809 - RR42_RS15595 0.14 -0.1 3,368,809 - RR42_RS15595 0.14 +0.1 3,368,809 - RR42_RS15595 0.14 -1.3 3,368,992 - RR42_RS15595 0.23 -1.1 3,369,473 + RR42_RS15595 0.46 -0.1 3,369,504 + RR42_RS15595 0.48 +0.7 3,369,504 + RR42_RS15595 0.48 +1.4 3,369,505 - RR42_RS15595 0.48 -0.0 3,369,577 - RR42_RS15595 0.51 -0.3 3,369,719 - RR42_RS15595 0.58 -0.5 3,369,771 + RR42_RS15595 0.61 -1.0 3,369,771 + RR42_RS15595 0.61 -0.5 3,369,772 - RR42_RS15595 0.61 -1.1 3,369,879 + RR42_RS15595 0.66 +0.3 3,370,122 + RR42_RS15595 0.78 -0.3 3,370,123 - RR42_RS15595 0.78 +0.1 3,370,240 - RR42_RS15595 0.84 -0.5 3,370,240 - RR42_RS15595 0.84 -0.0 3,370,240 - RR42_RS15595 0.84 +0.2 3,370,257 + RR42_RS15595 0.85 +0.2 3,370,257 + RR42_RS15595 0.85 -0.2 3,370,258 - RR42_RS15595 0.85 -0.2 3,370,258 - RR42_RS15595 0.85 -0.1 3,370,258 - RR42_RS15595 0.85 +0.1 3,370,258 - RR42_RS15595 0.85 -1.7 3,370,259 + RR42_RS15595 0.85 +0.1 3,370,260 - RR42_RS15595 0.85 -0.4 3,370,260 - RR42_RS15595 0.85 +0.3 3,370,509 + -0.2 3,370,510 - -0.0 3,370,742 + RR42_RS15600 0.15 +0.8 3,370,775 + RR42_RS15600 0.20 +0.0 3,370,775 + RR42_RS15600 0.20 +1.1 3,370,776 - RR42_RS15600 0.20 +0.7 3,370,776 - RR42_RS15600 0.20 -0.3 3,370,806 - RR42_RS15600 0.25 +2.1 3,370,806 - RR42_RS15600 0.25 -0.1 3,370,845 - RR42_RS15600 0.30 +0.0 3,371,079 - RR42_RS15600 0.66 -0.2 3,371,272 - -0.3 3,371,435 + -0.4 3,371,436 - +0.3 3,371,449 - -0.7 3,371,451 + +0.5 3,371,451 + -0.5 3,371,451 + +0.1 3,371,497 + +0.5
Or see this region's nucleotide sequence