Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13210 and RR42_RS13215 are separated by 261 nucleotides RR42_RS13215 and RR42_RS13220 overlap by 4 nucleotides
RR42_RS13210: RR42_RS13210 - porin, at 2,845,732 to 2,846,790
_RS13210
RR42_RS13215: RR42_RS13215 - transcriptional regulator, at 2,847,052 to 2,847,750
_RS13215
RR42_RS13220: RR42_RS13220 - histidine kinase, at 2,847,747 to 2,849,120
_RS13220
Position (kb)
2847
2848 Strain fitness (log2 ratio)
-1
0
1
2 at 2846.063 kb on + strand, within RR42_RS13210 at 2846.087 kb on + strand, within RR42_RS13210 at 2846.088 kb on - strand, within RR42_RS13210 at 2846.204 kb on + strand, within RR42_RS13210 at 2846.205 kb on - strand, within RR42_RS13210 at 2846.205 kb on - strand, within RR42_RS13210 at 2846.222 kb on + strand, within RR42_RS13210 at 2846.223 kb on - strand, within RR42_RS13210 at 2846.223 kb on - strand, within RR42_RS13210 at 2846.384 kb on + strand, within RR42_RS13210 at 2846.385 kb on - strand, within RR42_RS13210 at 2846.429 kb on + strand, within RR42_RS13210 at 2846.429 kb on + strand, within RR42_RS13210 at 2846.430 kb on - strand, within RR42_RS13210 at 2846.430 kb on - strand, within RR42_RS13210 at 2846.430 kb on - strand, within RR42_RS13210 at 2846.430 kb on - strand, within RR42_RS13210 at 2846.430 kb on - strand, within RR42_RS13210 at 2846.455 kb on + strand, within RR42_RS13210 at 2846.455 kb on + strand, within RR42_RS13210 at 2846.468 kb on + strand, within RR42_RS13210 at 2846.468 kb on + strand, within RR42_RS13210 at 2846.469 kb on - strand, within RR42_RS13210 at 2846.682 kb on - strand, within RR42_RS13210 at 2846.778 kb on - strand at 2846.867 kb on + strand at 2846.867 kb on + strand at 2846.867 kb on + strand at 2846.867 kb on + strand at 2846.868 kb on - strand at 2846.868 kb on - strand at 2846.868 kb on - strand at 2846.868 kb on - strand at 2846.868 kb on - strand at 2846.975 kb on + strand at 2846.975 kb on + strand at 2846.976 kb on - strand at 2846.976 kb on - strand at 2846.976 kb on - strand at 2846.976 kb on - strand at 2846.976 kb on - strand at 2847.138 kb on + strand, within RR42_RS13215 at 2847.138 kb on + strand, within RR42_RS13215 at 2847.138 kb on + strand, within RR42_RS13215 at 2847.138 kb on + strand, within RR42_RS13215 at 2847.138 kb on + strand, within RR42_RS13215 at 2847.138 kb on + strand, within RR42_RS13215 at 2847.139 kb on - strand, within RR42_RS13215 at 2847.139 kb on - strand, within RR42_RS13215 at 2847.139 kb on - strand, within RR42_RS13215 at 2847.139 kb on - strand, within RR42_RS13215 at 2847.139 kb on - strand, within RR42_RS13215 at 2847.344 kb on - strand, within RR42_RS13215 at 2847.398 kb on + strand, within RR42_RS13215 at 2847.452 kb on - strand, within RR42_RS13215 at 2847.452 kb on - strand, within RR42_RS13215 at 2847.628 kb on + strand, within RR42_RS13215 at 2847.628 kb on + strand, within RR42_RS13215 at 2847.629 kb on - strand, within RR42_RS13215 at 2847.698 kb on - strand at 2847.834 kb on + strand at 2847.834 kb on + strand at 2847.834 kb on + strand at 2847.835 kb on - strand at 2847.835 kb on - strand at 2847.835 kb on - strand at 2847.835 kb on - strand at 2847.835 kb on - strand at 2847.952 kb on - strand, within RR42_RS13220 at 2847.952 kb on - strand, within RR42_RS13220 at 2847.996 kb on + strand, within RR42_RS13220 at 2847.996 kb on + strand, within RR42_RS13220 at 2847.996 kb on + strand, within RR42_RS13220 at 2847.997 kb on - strand, within RR42_RS13220 at 2847.997 kb on - strand, within RR42_RS13220 at 2847.999 kb on + strand, within RR42_RS13220 at 2847.999 kb on + strand, within RR42_RS13220 at 2847.999 kb on + strand, within RR42_RS13220 at 2847.999 kb on + strand, within RR42_RS13220 at 2848.000 kb on - strand, within RR42_RS13220 at 2848.000 kb on - strand, within RR42_RS13220 at 2848.000 kb on - strand, within RR42_RS13220 at 2848.038 kb on + strand, within RR42_RS13220 at 2848.080 kb on + strand, within RR42_RS13220 at 2848.080 kb on + strand, within RR42_RS13220 at 2848.096 kb on - strand, within RR42_RS13220 at 2848.437 kb on + strand, within RR42_RS13220 at 2848.437 kb on + strand, within RR42_RS13220 at 2848.437 kb on + strand, within RR42_RS13220 at 2848.438 kb on - strand, within RR42_RS13220 at 2848.586 kb on - strand, within RR42_RS13220 at 2848.700 kb on - strand, within RR42_RS13220 at 2848.700 kb on - strand, within RR42_RS13220 at 2848.703 kb on + strand, within RR42_RS13220 at 2848.703 kb on + strand, within RR42_RS13220 at 2848.703 kb on + strand, within RR42_RS13220 at 2848.704 kb on - strand, within RR42_RS13220 at 2848.704 kb on - strand, within RR42_RS13220 at 2848.704 kb on - strand, within RR42_RS13220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,846,063 + RR42_RS13210 0.31 +0.6 2,846,087 + RR42_RS13210 0.34 +0.5 2,846,088 - RR42_RS13210 0.34 -0.6 2,846,204 + RR42_RS13210 0.45 +0.0 2,846,205 - RR42_RS13210 0.45 +0.7 2,846,205 - RR42_RS13210 0.45 -0.0 2,846,222 + RR42_RS13210 0.46 +1.0 2,846,223 - RR42_RS13210 0.46 +0.9 2,846,223 - RR42_RS13210 0.46 +0.3 2,846,384 + RR42_RS13210 0.62 +0.5 2,846,385 - RR42_RS13210 0.62 -0.2 2,846,429 + RR42_RS13210 0.66 +1.7 2,846,429 + RR42_RS13210 0.66 -0.3 2,846,430 - RR42_RS13210 0.66 +0.3 2,846,430 - RR42_RS13210 0.66 +0.7 2,846,430 - RR42_RS13210 0.66 -0.2 2,846,430 - RR42_RS13210 0.66 +1.0 2,846,430 - RR42_RS13210 0.66 +2.0 2,846,455 + RR42_RS13210 0.68 +0.7 2,846,455 + RR42_RS13210 0.68 +0.6 2,846,468 + RR42_RS13210 0.69 +0.5 2,846,468 + RR42_RS13210 0.69 +0.5 2,846,469 - RR42_RS13210 0.70 +0.1 2,846,682 - RR42_RS13210 0.90 +0.6 2,846,778 - -1.6 2,846,867 + +0.4 2,846,867 + -0.3 2,846,867 + +1.2 2,846,867 + -0.5 2,846,868 - +0.7 2,846,868 - +0.7 2,846,868 - +0.6 2,846,868 - +0.9 2,846,868 - +0.1 2,846,975 + +0.2 2,846,975 + +0.2 2,846,976 - -1.4 2,846,976 - +0.6 2,846,976 - -0.8 2,846,976 - -0.2 2,846,976 - +0.2 2,847,138 + RR42_RS13215 0.12 -0.5 2,847,138 + RR42_RS13215 0.12 -0.7 2,847,138 + RR42_RS13215 0.12 -0.0 2,847,138 + RR42_RS13215 0.12 -0.5 2,847,138 + RR42_RS13215 0.12 +1.2 2,847,138 + RR42_RS13215 0.12 +0.7 2,847,139 - RR42_RS13215 0.12 -0.1 2,847,139 - RR42_RS13215 0.12 -0.2 2,847,139 - RR42_RS13215 0.12 +1.2 2,847,139 - RR42_RS13215 0.12 +0.9 2,847,139 - RR42_RS13215 0.12 +1.8 2,847,344 - RR42_RS13215 0.42 -0.5 2,847,398 + RR42_RS13215 0.49 +0.9 2,847,452 - RR42_RS13215 0.57 +0.6 2,847,452 - RR42_RS13215 0.57 +1.0 2,847,628 + RR42_RS13215 0.82 +1.0 2,847,628 + RR42_RS13215 0.82 -0.4 2,847,629 - RR42_RS13215 0.83 -0.3 2,847,698 - -0.1 2,847,834 + +0.8 2,847,834 + +1.9 2,847,834 + -0.7 2,847,835 - +1.1 2,847,835 - +0.1 2,847,835 - +1.4 2,847,835 - +0.8 2,847,835 - +1.0 2,847,952 - RR42_RS13220 0.15 +0.2 2,847,952 - RR42_RS13220 0.15 +0.8 2,847,996 + RR42_RS13220 0.18 -0.8 2,847,996 + RR42_RS13220 0.18 +0.6 2,847,996 + RR42_RS13220 0.18 +0.6 2,847,997 - RR42_RS13220 0.18 +0.3 2,847,997 - RR42_RS13220 0.18 +0.3 2,847,999 + RR42_RS13220 0.18 +0.4 2,847,999 + RR42_RS13220 0.18 +0.3 2,847,999 + RR42_RS13220 0.18 +0.1 2,847,999 + RR42_RS13220 0.18 +0.5 2,848,000 - RR42_RS13220 0.18 +0.4 2,848,000 - RR42_RS13220 0.18 +0.5 2,848,000 - RR42_RS13220 0.18 -0.2 2,848,038 + RR42_RS13220 0.21 +0.2 2,848,080 + RR42_RS13220 0.24 +0.4 2,848,080 + RR42_RS13220 0.24 +0.5 2,848,096 - RR42_RS13220 0.25 +0.0 2,848,437 + RR42_RS13220 0.50 +0.7 2,848,437 + RR42_RS13220 0.50 -1.4 2,848,437 + RR42_RS13220 0.50 -0.9 2,848,438 - RR42_RS13220 0.50 +0.4 2,848,586 - RR42_RS13220 0.61 +1.4 2,848,700 - RR42_RS13220 0.69 +0.6 2,848,700 - RR42_RS13220 0.69 +0.7 2,848,703 + RR42_RS13220 0.70 +0.2 2,848,703 + RR42_RS13220 0.70 +0.3 2,848,703 + RR42_RS13220 0.70 +0.1 2,848,704 - RR42_RS13220 0.70 +0.5 2,848,704 - RR42_RS13220 0.70 +0.3 2,848,704 - RR42_RS13220 0.70 +0.5
Or see this region's nucleotide sequence