Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11115 and RR42_RS11120 overlap by 11 nucleotides RR42_RS11120 and RR42_RS11125 are separated by 230 nucleotides RR42_RS11125 and RR42_RS11130 are separated by 74 nucleotides RR42_RS11130 and RR42_RS11135 overlap by 1 nucleotides
RR42_RS11115: RR42_RS11115 - ornithine cyclodeaminase, at 2,374,012 to 2,374,983
_RS11115
RR42_RS11120: RR42_RS11120 - DNA-binding protein, at 2,374,973 to 2,375,614
_RS11120
RR42_RS11125: RR42_RS11125 - glycerophosphodiester phosphodiesterase, at 2,375,845 to 2,377,218
_RS11125
RR42_RS11130: RR42_RS11130 - mannosyltransferase, at 2,377,293 to 2,378,006
_RS11130
RR42_RS11135: RR42_RS11135 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,378,006 to 2,378,779
_RS11135
Position (kb)
2375
2376
2377
2378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.879 kb on - strand, within RR42_RS11115 at 2374.983 kb on + strand at 2374.984 kb on - strand at 2374.987 kb on + strand at 2374.987 kb on + strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.988 kb on - strand at 2374.989 kb on + strand at 2374.989 kb on + strand at 2374.990 kb on - strand at 2375.293 kb on - strand, within RR42_RS11120 at 2375.364 kb on + strand, within RR42_RS11120 at 2375.365 kb on - strand, within RR42_RS11120 at 2375.386 kb on - strand, within RR42_RS11120 at 2375.603 kb on - strand at 2375.603 kb on - strand at 2375.704 kb on + strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.705 kb on - strand at 2375.843 kb on - strand at 2375.843 kb on - strand at 2375.989 kb on + strand, within RR42_RS11125 at 2375.990 kb on - strand, within RR42_RS11125 at 2375.990 kb on - strand, within RR42_RS11125 at 2375.992 kb on - strand, within RR42_RS11125 at 2375.992 kb on - strand, within RR42_RS11125 at 2376.250 kb on + strand, within RR42_RS11125 at 2376.250 kb on + strand, within RR42_RS11125 at 2376.251 kb on - strand, within RR42_RS11125 at 2376.251 kb on - strand, within RR42_RS11125 at 2376.253 kb on + strand, within RR42_RS11125 at 2376.361 kb on + strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125 at 2376.362 kb on - strand, within RR42_RS11125 at 2376.931 kb on + strand, within RR42_RS11125 at 2376.932 kb on - strand, within RR42_RS11125 at 2377.216 kb on + strand at 2377.217 kb on - strand at 2377.294 kb on + strand at 2377.295 kb on - strand at 2377.472 kb on - strand, within RR42_RS11130 at 2377.611 kb on - strand, within RR42_RS11130 at 2377.645 kb on + strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.646 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.740 kb on - strand, within RR42_RS11130 at 2377.759 kb on + strand, within RR42_RS11130 at 2377.925 kb on + strand, within RR42_RS11130 at 2377.950 kb on - strand at 2377.967 kb on - strand at 2378.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 2,374,879 - RR42_RS11115 0.89 -0.3 2,374,879 - RR42_RS11115 0.89 +0.2 2,374,983 + +0.2 2,374,984 - +0.3 2,374,987 + -1.7 2,374,987 + -0.5 2,374,988 - +0.6 2,374,988 - +0.9 2,374,988 - +1.8 2,374,988 - -0.0 2,374,988 - -0.0 2,374,989 + -0.6 2,374,989 + -0.8 2,374,990 - -1.1 2,375,293 - RR42_RS11120 0.50 -0.8 2,375,364 + RR42_RS11120 0.61 -0.9 2,375,365 - RR42_RS11120 0.61 -2.1 2,375,386 - RR42_RS11120 0.64 -1.2 2,375,603 - -0.8 2,375,603 - -1.5 2,375,704 + -0.3 2,375,705 - -0.9 2,375,705 - -0.4 2,375,705 - +0.7 2,375,843 - -1.5 2,375,843 - +0.6 2,375,989 + RR42_RS11125 0.10 -0.2 2,375,990 - RR42_RS11125 0.11 -0.4 2,375,990 - RR42_RS11125 0.11 -0.4 2,375,992 - RR42_RS11125 0.11 +0.6 2,375,992 - RR42_RS11125 0.11 -0.2 2,376,250 + RR42_RS11125 0.29 +0.3 2,376,250 + RR42_RS11125 0.29 +0.8 2,376,251 - RR42_RS11125 0.30 -0.8 2,376,251 - RR42_RS11125 0.30 -2.2 2,376,253 + RR42_RS11125 0.30 +1.4 2,376,361 + RR42_RS11125 0.38 -0.3 2,376,362 - RR42_RS11125 0.38 +0.3 2,376,362 - RR42_RS11125 0.38 -0.2 2,376,931 + RR42_RS11125 0.79 -0.2 2,376,932 - RR42_RS11125 0.79 -0.7 2,377,216 + +1.5 2,377,217 - +0.0 2,377,294 + -1.2 2,377,295 - -0.0 2,377,472 - RR42_RS11130 0.25 -0.2 2,377,611 - RR42_RS11130 0.45 -0.1 2,377,645 + RR42_RS11130 0.49 +0.5 2,377,646 - RR42_RS11130 0.49 -1.1 2,377,646 - RR42_RS11130 0.49 +0.8 2,377,646 - RR42_RS11130 0.49 +1.4 2,377,646 - RR42_RS11130 0.49 +0.2 2,377,646 - RR42_RS11130 0.49 -0.4 2,377,646 - RR42_RS11130 0.49 -0.4 2,377,646 - RR42_RS11130 0.49 +0.6 2,377,740 - RR42_RS11130 0.63 -0.2 2,377,740 - RR42_RS11130 0.63 -0.9 2,377,759 + RR42_RS11130 0.65 +0.4 2,377,925 + RR42_RS11130 0.89 +0.3 2,377,950 - +0.7 2,377,967 - -0.4 2,378,052 + -0.8
Or see this region's nucleotide sequence