Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS10650

Experiment: R2A with Nickel (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS10640 and RR42_RS10645 are separated by 154 nucleotidesRR42_RS10645 and RR42_RS10650 are separated by 177 nucleotidesRR42_RS10650 and RR42_RS10655 overlap by 4 nucleotides RR42_RS10640: RR42_RS10640 - alanine dehydrogenase, at 2,273,201 to 2,274,316 _RS10640 RR42_RS10645: RR42_RS10645 - hypothetical protein, at 2,274,471 to 2,274,818 _RS10645 RR42_RS10650: RR42_RS10650 - acetyl-CoA synthetase, at 2,274,996 to 2,276,762 _RS10650 RR42_RS10655: RR42_RS10655 - pyruvate dehydrogenase, at 2,276,759 to 2,277,781 _RS10655 Position (kb) 2274 2275 2276 2277Strain fitness (log2 ratio) -3 -2 -1 0 1at 2274.037 kb on - strand, within RR42_RS10640at 2274.037 kb on - strand, within RR42_RS10640at 2274.043 kb on - strand, within RR42_RS10640at 2274.125 kb on + strand, within RR42_RS10640at 2274.146 kb on + strand, within RR42_RS10640at 2274.146 kb on + strand, within RR42_RS10640at 2274.147 kb on - strand, within RR42_RS10640at 2274.169 kb on - strand, within RR42_RS10640at 2274.251 kb on - strandat 2274.472 kb on + strandat 2274.472 kb on + strandat 2274.473 kb on - strandat 2274.549 kb on + strand, within RR42_RS10645at 2274.549 kb on + strand, within RR42_RS10645at 2274.654 kb on + strand, within RR42_RS10645at 2274.654 kb on + strand, within RR42_RS10645at 2274.655 kb on - strand, within RR42_RS10645at 2274.655 kb on - strand, within RR42_RS10645at 2274.656 kb on + strand, within RR42_RS10645at 2274.657 kb on - strand, within RR42_RS10645at 2274.657 kb on - strand, within RR42_RS10645at 2274.751 kb on - strand, within RR42_RS10645at 2274.751 kb on - strand, within RR42_RS10645at 2274.767 kb on - strand, within RR42_RS10645at 2274.767 kb on - strand, within RR42_RS10645at 2274.894 kb on - strandat 2274.894 kb on - strandat 2274.895 kb on + strandat 2274.895 kb on + strandat 2274.895 kb on + strandat 2274.895 kb on + strandat 2274.895 kb on + strandat 2274.895 kb on + strandat 2274.896 kb on - strandat 2274.959 kb on - strandat 2274.966 kb on + strandat 2274.967 kb on - strandat 2274.967 kb on - strandat 2275.175 kb on + strand, within RR42_RS10650at 2275.239 kb on + strand, within RR42_RS10650at 2275.348 kb on - strand, within RR42_RS10650at 2275.654 kb on + strand, within RR42_RS10650at 2275.655 kb on - strand, within RR42_RS10650at 2275.773 kb on + strand, within RR42_RS10650at 2275.773 kb on + strand, within RR42_RS10650at 2275.773 kb on + strand, within RR42_RS10650at 2275.782 kb on - strand, within RR42_RS10650at 2275.815 kb on + strand, within RR42_RS10650at 2275.890 kb on + strand, within RR42_RS10650at 2275.926 kb on + strand, within RR42_RS10650at 2275.926 kb on + strand, within RR42_RS10650at 2275.986 kb on + strand, within RR42_RS10650at 2276.228 kb on - strand, within RR42_RS10650at 2276.228 kb on - strand, within RR42_RS10650at 2276.230 kb on + strand, within RR42_RS10650at 2276.231 kb on - strand, within RR42_RS10650at 2276.231 kb on - strand, within RR42_RS10650at 2276.382 kb on + strand, within RR42_RS10650at 2276.383 kb on - strand, within RR42_RS10650at 2276.386 kb on - strand, within RR42_RS10650at 2276.386 kb on - strand, within RR42_RS10650at 2276.386 kb on - strand, within RR42_RS10650at 2276.518 kb on + strand, within RR42_RS10650at 2276.519 kb on - strand, within RR42_RS10650at 2276.736 kb on - strandat 2276.816 kb on - strandat 2276.889 kb on - strand, within RR42_RS10655at 2276.904 kb on + strand, within RR42_RS10655at 2276.905 kb on - strand, within RR42_RS10655at 2276.924 kb on + strand, within RR42_RS10655at 2276.924 kb on + strand, within RR42_RS10655at 2276.925 kb on - strand, within RR42_RS10655at 2276.996 kb on + strand, within RR42_RS10655at 2276.997 kb on - strand, within RR42_RS10655at 2277.059 kb on + strand, within RR42_RS10655at 2277.395 kb on + strand, within RR42_RS10655at 2277.396 kb on - strand, within RR42_RS10655at 2277.401 kb on - strand, within RR42_RS10655at 2277.401 kb on - strand, within RR42_RS10655at 2277.401 kb on - strand, within RR42_RS10655at 2277.515 kb on + strand, within RR42_RS10655at 2277.516 kb on - strand, within RR42_RS10655at 2277.540 kb on - strand, within RR42_RS10655at 2277.554 kb on + strand, within RR42_RS10655at 2277.604 kb on + strand, within RR42_RS10655at 2277.605 kb on - strand, within RR42_RS10655at 2277.605 kb on - strand, within RR42_RS10655at 2277.755 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM
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2,274,037 - RR42_RS10640 0.75 -0.1
2,274,037 - RR42_RS10640 0.75 -0.2
2,274,043 - RR42_RS10640 0.75 -0.3
2,274,125 + RR42_RS10640 0.83 -0.0
2,274,146 + RR42_RS10640 0.85 -0.6
2,274,146 + RR42_RS10640 0.85 -0.2
2,274,147 - RR42_RS10640 0.85 -0.1
2,274,169 - RR42_RS10640 0.87 -0.3
2,274,251 - +0.5
2,274,472 + -0.3
2,274,472 + -0.9
2,274,473 - +0.4
2,274,549 + RR42_RS10645 0.22 -0.1
2,274,549 + RR42_RS10645 0.22 -0.0
2,274,654 + RR42_RS10645 0.53 -0.5
2,274,654 + RR42_RS10645 0.53 +0.1
2,274,655 - RR42_RS10645 0.53 -0.1
2,274,655 - RR42_RS10645 0.53 +0.9
2,274,656 + RR42_RS10645 0.53 -0.5
2,274,657 - RR42_RS10645 0.53 -1.3
2,274,657 - RR42_RS10645 0.53 +0.4
2,274,751 - RR42_RS10645 0.80 -0.4
2,274,751 - RR42_RS10645 0.80 -0.1
2,274,767 - RR42_RS10645 0.85 +0.0
2,274,767 - RR42_RS10645 0.85 +0.9
2,274,894 - +0.3
2,274,894 - +1.4
2,274,895 + +1.0
2,274,895 + +0.2
2,274,895 + -0.1
2,274,895 + -0.3
2,274,895 + -2.8
2,274,895 + -0.0
2,274,896 - +0.0
2,274,959 - -1.6
2,274,966 + -0.2
2,274,967 - -0.7
2,274,967 - -2.0
2,275,175 + RR42_RS10650 0.10 -1.0
2,275,239 + RR42_RS10650 0.14 +0.2
2,275,348 - RR42_RS10650 0.20 -0.7
2,275,654 + RR42_RS10650 0.37 -0.1
2,275,655 - RR42_RS10650 0.37 +1.1
2,275,773 + RR42_RS10650 0.44 +0.2
2,275,773 + RR42_RS10650 0.44 -0.2
2,275,773 + RR42_RS10650 0.44 +0.1
2,275,782 - RR42_RS10650 0.44 -0.6
2,275,815 + RR42_RS10650 0.46 -0.1
2,275,890 + RR42_RS10650 0.51 +0.4
2,275,926 + RR42_RS10650 0.53 -0.7
2,275,926 + RR42_RS10650 0.53 -0.3
2,275,986 + RR42_RS10650 0.56 -0.1
2,276,228 - RR42_RS10650 0.70 -0.9
2,276,228 - RR42_RS10650 0.70 -0.3
2,276,230 + RR42_RS10650 0.70 -1.9
2,276,231 - RR42_RS10650 0.70 -0.0
2,276,231 - RR42_RS10650 0.70 -1.0
2,276,382 + RR42_RS10650 0.78 +0.1
2,276,383 - RR42_RS10650 0.78 +0.0
2,276,386 - RR42_RS10650 0.79 -1.2
2,276,386 - RR42_RS10650 0.79 +0.2
2,276,386 - RR42_RS10650 0.79 -0.2
2,276,518 + RR42_RS10650 0.86 -0.5
2,276,519 - RR42_RS10650 0.86 -0.7
2,276,736 - +0.3
2,276,816 - -0.0
2,276,889 - RR42_RS10655 0.13 +1.4
2,276,904 + RR42_RS10655 0.14 +0.6
2,276,905 - RR42_RS10655 0.14 -0.3
2,276,924 + RR42_RS10655 0.16 +0.4
2,276,924 + RR42_RS10655 0.16 +0.5
2,276,925 - RR42_RS10655 0.16 -0.1
2,276,996 + RR42_RS10655 0.23 -0.1
2,276,997 - RR42_RS10655 0.23 -0.6
2,277,059 + RR42_RS10655 0.29 +0.7
2,277,395 + RR42_RS10655 0.62 -0.5
2,277,396 - RR42_RS10655 0.62 +0.0
2,277,401 - RR42_RS10655 0.63 -0.9
2,277,401 - RR42_RS10655 0.63 -0.9
2,277,401 - RR42_RS10655 0.63 -0.0
2,277,515 + RR42_RS10655 0.74 +0.9
2,277,516 - RR42_RS10655 0.74 -1.2
2,277,540 - RR42_RS10655 0.76 -0.5
2,277,554 + RR42_RS10655 0.78 +0.1
2,277,604 + RR42_RS10655 0.83 +0.8
2,277,605 - RR42_RS10655 0.83 +0.0
2,277,605 - RR42_RS10655 0.83 +0.5
2,277,755 + -0.2

Or see this region's nucleotide sequence