Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS06015

Experiment: R2A with Nickel (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS06005 and RR42_RS06010 are separated by 213 nucleotidesRR42_RS06010 and RR42_RS06015 are separated by 48 nucleotidesRR42_RS06015 and RR42_RS06020 are separated by 80 nucleotidesRR42_RS06020 and RR42_RS06025 are separated by 68 nucleotides RR42_RS06005: RR42_RS06005 - hypothetical protein, at 1,320,695 to 1,321,030 _RS06005 RR42_RS06010: RR42_RS06010 - phosphotyrosine protein phosphatase, at 1,321,244 to 1,321,735 _RS06010 RR42_RS06015: RR42_RS06015 - recombinase, at 1,321,784 to 1,323,874 _RS06015 RR42_RS06020: RR42_RS06020 - Fe-S cluster assembly transcriptional regulator IscR, at 1,323,955 to 1,324,509 _RS06020 RR42_RS06025: RR42_RS06025 - cysteine desulfurase, at 1,324,578 to 1,325,795 _RS06025 Position (kb) 1321 1322 1323 1324Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1321.040 kb on - strandat 1321.096 kb on - strandat 1321.254 kb on - strandat 1321.284 kb on + strandat 1321.284 kb on + strandat 1321.284 kb on + strandat 1321.284 kb on + strandat 1321.285 kb on - strandat 1321.285 kb on - strandat 1321.285 kb on - strandat 1321.382 kb on + strand, within RR42_RS06010at 1321.383 kb on - strand, within RR42_RS06010at 1321.383 kb on - strand, within RR42_RS06010at 1321.383 kb on - strand, within RR42_RS06010at 1321.440 kb on - strand, within RR42_RS06010at 1321.628 kb on + strand, within RR42_RS06010at 1321.632 kb on - strand, within RR42_RS06010at 1321.667 kb on + strand, within RR42_RS06010at 1321.667 kb on + strand, within RR42_RS06010at 1321.668 kb on - strand, within RR42_RS06010at 1321.668 kb on - strand, within RR42_RS06010at 1321.668 kb on - strand, within RR42_RS06010at 1321.768 kb on - strandat 1321.994 kb on + strand, within RR42_RS06015at 1321.994 kb on + strand, within RR42_RS06015at 1322.189 kb on - strand, within RR42_RS06015at 1322.215 kb on + strand, within RR42_RS06015at 1322.291 kb on - strand, within RR42_RS06015at 1322.543 kb on + strand, within RR42_RS06015at 1322.591 kb on + strand, within RR42_RS06015at 1322.592 kb on - strand, within RR42_RS06015at 1322.592 kb on - strand, within RR42_RS06015at 1322.686 kb on + strand, within RR42_RS06015at 1322.846 kb on + strand, within RR42_RS06015at 1322.847 kb on - strand, within RR42_RS06015at 1322.927 kb on + strand, within RR42_RS06015at 1322.928 kb on - strand, within RR42_RS06015at 1322.945 kb on + strand, within RR42_RS06015at 1322.945 kb on + strand, within RR42_RS06015at 1322.946 kb on - strand, within RR42_RS06015at 1322.946 kb on - strand, within RR42_RS06015at 1322.996 kb on + strand, within RR42_RS06015at 1322.997 kb on - strand, within RR42_RS06015at 1322.997 kb on - strand, within RR42_RS06015at 1322.997 kb on - strand, within RR42_RS06015at 1322.997 kb on - strand, within RR42_RS06015at 1322.997 kb on - strand, within RR42_RS06015at 1323.134 kb on - strand, within RR42_RS06015at 1323.134 kb on - strand, within RR42_RS06015at 1323.134 kb on - strand, within RR42_RS06015at 1323.134 kb on - strand, within RR42_RS06015at 1323.134 kb on - strand, within RR42_RS06015at 1323.331 kb on - strand, within RR42_RS06015at 1323.410 kb on + strand, within RR42_RS06015at 1323.410 kb on + strand, within RR42_RS06015at 1323.411 kb on - strand, within RR42_RS06015at 1323.411 kb on - strand, within RR42_RS06015at 1323.411 kb on - strand, within RR42_RS06015at 1323.428 kb on + strand, within RR42_RS06015at 1323.428 kb on + strand, within RR42_RS06015at 1323.429 kb on - strand, within RR42_RS06015at 1323.481 kb on + strand, within RR42_RS06015at 1323.481 kb on + strand, within RR42_RS06015at 1323.482 kb on - strand, within RR42_RS06015at 1323.482 kb on - strand, within RR42_RS06015at 1324.248 kb on + strand, within RR42_RS06020

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM
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1,321,040 - -0.7
1,321,096 - +0.4
1,321,254 - -0.3
1,321,284 + -0.1
1,321,284 + -2.4
1,321,284 + +0.6
1,321,284 + +0.0
1,321,285 - -0.3
1,321,285 - -0.4
1,321,285 - +1.8
1,321,382 + RR42_RS06010 0.28 -0.1
1,321,383 - RR42_RS06010 0.28 -0.6
1,321,383 - RR42_RS06010 0.28 -1.0
1,321,383 - RR42_RS06010 0.28 -0.9
1,321,440 - RR42_RS06010 0.40 -0.6
1,321,628 + RR42_RS06010 0.78 +0.5
1,321,632 - RR42_RS06010 0.79 +0.7
1,321,667 + RR42_RS06010 0.86 +0.2
1,321,667 + RR42_RS06010 0.86 -0.1
1,321,668 - RR42_RS06010 0.86 -0.8
1,321,668 - RR42_RS06010 0.86 +0.6
1,321,668 - RR42_RS06010 0.86 +0.4
1,321,768 - -0.3
1,321,994 + RR42_RS06015 0.10 +0.1
1,321,994 + RR42_RS06015 0.10 +1.3
1,322,189 - RR42_RS06015 0.19 +0.2
1,322,215 + RR42_RS06015 0.21 -0.2
1,322,291 - RR42_RS06015 0.24 +0.2
1,322,543 + RR42_RS06015 0.36 +0.8
1,322,591 + RR42_RS06015 0.39 -0.2
1,322,592 - RR42_RS06015 0.39 +0.6
1,322,592 - RR42_RS06015 0.39 +0.2
1,322,686 + RR42_RS06015 0.43 -0.8
1,322,846 + RR42_RS06015 0.51 -0.9
1,322,847 - RR42_RS06015 0.51 -1.8
1,322,927 + RR42_RS06015 0.55 -0.4
1,322,928 - RR42_RS06015 0.55 -0.9
1,322,945 + RR42_RS06015 0.56 +0.2
1,322,945 + RR42_RS06015 0.56 -0.2
1,322,946 - RR42_RS06015 0.56 -0.1
1,322,946 - RR42_RS06015 0.56 +0.8
1,322,996 + RR42_RS06015 0.58 -0.4
1,322,997 - RR42_RS06015 0.58 -2.7
1,322,997 - RR42_RS06015 0.58 +1.5
1,322,997 - RR42_RS06015 0.58 +0.3
1,322,997 - RR42_RS06015 0.58 +0.5
1,322,997 - RR42_RS06015 0.58 +0.2
1,323,134 - RR42_RS06015 0.65 +0.1
1,323,134 - RR42_RS06015 0.65 +0.3
1,323,134 - RR42_RS06015 0.65 -1.0
1,323,134 - RR42_RS06015 0.65 -0.5
1,323,134 - RR42_RS06015 0.65 +0.2
1,323,331 - RR42_RS06015 0.74 +1.6
1,323,410 + RR42_RS06015 0.78 -0.6
1,323,410 + RR42_RS06015 0.78 -0.7
1,323,411 - RR42_RS06015 0.78 -0.3
1,323,411 - RR42_RS06015 0.78 +0.8
1,323,411 - RR42_RS06015 0.78 -0.4
1,323,428 + RR42_RS06015 0.79 -0.6
1,323,428 + RR42_RS06015 0.79 -0.2
1,323,429 - RR42_RS06015 0.79 +0.4
1,323,481 + RR42_RS06015 0.81 +0.3
1,323,481 + RR42_RS06015 0.81 -0.5
1,323,482 - RR42_RS06015 0.81 +1.1
1,323,482 - RR42_RS06015 0.81 +1.2
1,324,248 + RR42_RS06020 0.53 -4.2

Or see this region's nucleotide sequence