Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS04525

Experiment: R2A with Nickel (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS04520 and RR42_RS04525 are separated by 200 nucleotidesRR42_RS04525 and RR42_RS04530 are separated by 284 nucleotides RR42_RS04520: RR42_RS04520 - ADP-L-glycero-D-manno-heptose-6-epimerase, at 1,009,490 to 1,010,485 _RS04520 RR42_RS04525: RR42_RS04525 - hypothetical protein, at 1,010,686 to 1,011,048 _RS04525 RR42_RS04530: RR42_RS04530 - pyrimidine permease, at 1,011,333 to 1,012,649 _RS04530 Position (kb) 1010 1011 1012Strain fitness (log2 ratio) -1 0 1at 1010.505 kb on + strandat 1010.505 kb on + strandat 1010.506 kb on - strandat 1010.605 kb on + strandat 1010.606 kb on - strandat 1010.658 kb on - strandat 1010.772 kb on + strand, within RR42_RS04525at 1011.046 kb on + strandat 1011.047 kb on - strandat 1011.088 kb on + strandat 1011.176 kb on - strandat 1011.296 kb on + strandat 1011.441 kb on - strandat 1011.455 kb on - strandat 1011.579 kb on + strand, within RR42_RS04530at 1011.579 kb on + strand, within RR42_RS04530at 1011.580 kb on - strand, within RR42_RS04530at 1011.580 kb on - strand, within RR42_RS04530at 1011.699 kb on + strand, within RR42_RS04530at 1011.700 kb on - strand, within RR42_RS04530at 1011.700 kb on - strand, within RR42_RS04530at 1011.700 kb on - strand, within RR42_RS04530at 1011.700 kb on - strand, within RR42_RS04530at 1011.848 kb on + strand, within RR42_RS04530at 1011.929 kb on - strand, within RR42_RS04530at 1011.964 kb on - strand, within RR42_RS04530at 1011.964 kb on - strand, within RR42_RS04530

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM
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1,010,505 + +0.0
1,010,505 + +0.1
1,010,506 - -0.2
1,010,605 + +0.0
1,010,606 - +0.1
1,010,658 - -1.0
1,010,772 + RR42_RS04525 0.24 +0.5
1,011,046 + -0.6
1,011,047 - +0.1
1,011,088 + +0.1
1,011,176 - -0.9
1,011,296 + -0.1
1,011,441 - -0.3
1,011,455 - +0.0
1,011,579 + RR42_RS04530 0.19 -0.1
1,011,579 + RR42_RS04530 0.19 -1.3
1,011,580 - RR42_RS04530 0.19 -0.8
1,011,580 - RR42_RS04530 0.19 +0.1
1,011,699 + RR42_RS04530 0.28 +0.2
1,011,700 - RR42_RS04530 0.28 -0.3
1,011,700 - RR42_RS04530 0.28 -0.7
1,011,700 - RR42_RS04530 0.28 -0.0
1,011,700 - RR42_RS04530 0.28 -1.4
1,011,848 + RR42_RS04530 0.39 -1.4
1,011,929 - RR42_RS04530 0.45 -0.1
1,011,964 - RR42_RS04530 0.48 +0.3
1,011,964 - RR42_RS04530 0.48 -0.2

Or see this region's nucleotide sequence