Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04315 and RR42_RS04320 are separated by 170 nucleotides RR42_RS04320 and RR42_RS04325 are separated by 133 nucleotides RR42_RS04325 and RR42_RS04330 are separated by 23 nucleotides
RR42_RS04315: RR42_RS04315 - thioredoxin reductase, at 957,970 to 958,917
_RS04315
RR42_RS04320: RR42_RS04320 - cell division protein FtsK, at 959,088 to 961,412
_RS04320
RR42_RS04325: RR42_RS04325 - membrane protein, at 961,546 to 962,184
_RS04325
RR42_RS04330: RR42_RS04330 - lipoprotein, at 962,208 to 962,612
_RS04330
Position (kb) 959
960
961
962 Strain fitness (log2 ratio) -2
-1
0
1 at 958.978 kb on + strand at 958.978 kb on + strand at 958.978 kb on + strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 958.979 kb on - strand at 959.041 kb on + strand at 959.042 kb on - strand at 959.042 kb on - strand at 960.196 kb on - strand, within RR42_RS04320 at 960.196 kb on - strand, within RR42_RS04320 at 960.426 kb on + strand, within RR42_RS04320 at 960.426 kb on + strand, within RR42_RS04320 at 960.427 kb on - strand, within RR42_RS04320 at 960.492 kb on + strand, within RR42_RS04320 at 960.492 kb on + strand, within RR42_RS04320 at 960.630 kb on + strand, within RR42_RS04320 at 961.002 kb on + strand, within RR42_RS04320 at 961.002 kb on + strand, within RR42_RS04320 at 961.002 kb on + strand, within RR42_RS04320 at 961.110 kb on + strand, within RR42_RS04320 at 961.111 kb on - strand, within RR42_RS04320 at 961.410 kb on + strand at 961.411 kb on - strand at 961.411 kb on - strand at 961.424 kb on + strand at 961.424 kb on + strand at 961.435 kb on + strand at 961.828 kb on + strand, within RR42_RS04325 at 961.828 kb on + strand, within RR42_RS04325 at 961.828 kb on + strand, within RR42_RS04325 at 961.834 kb on + strand, within RR42_RS04325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 958,978 + +0.1 958,978 + -1.5 958,978 + -0.5 958,979 - +1.4 958,979 - -0.4 958,979 - +1.3 958,979 - +0.0 958,979 - -0.3 958,979 - +0.6 959,041 + +0.4 959,042 - +0.7 959,042 - +0.5 960,196 - RR42_RS04320 0.48 -0.7 960,196 - RR42_RS04320 0.48 -0.8 960,426 + RR42_RS04320 0.58 -0.9 960,426 + RR42_RS04320 0.58 -0.2 960,427 - RR42_RS04320 0.58 -2.0 960,492 + RR42_RS04320 0.60 -0.8 960,492 + RR42_RS04320 0.60 -1.6 960,630 + RR42_RS04320 0.66 -1.9 961,002 + RR42_RS04320 0.82 -0.3 961,002 + RR42_RS04320 0.82 -0.7 961,002 + RR42_RS04320 0.82 -0.8 961,110 + RR42_RS04320 0.87 +0.5 961,111 - RR42_RS04320 0.87 -2.0 961,410 + -0.0 961,411 - +0.1 961,411 - -1.4 961,424 + -0.5 961,424 + -1.0 961,435 + +0.0 961,828 + RR42_RS04325 0.44 -1.7 961,828 + RR42_RS04325 0.44 -1.1 961,828 + RR42_RS04325 0.44 -1.7 961,834 + RR42_RS04325 0.45 -2.7
Or see this region's nucleotide sequence