Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03140 and RR42_RS03145 are separated by 94 nucleotides RR42_RS03145 and RR42_RS03150 are separated by 48 nucleotides RR42_RS03150 and RR42_RS03155 are separated by 77 nucleotides
RR42_RS03140: RR42_RS03140 - molybdenum cofactor biosynthesis protein MoaC, at 694,065 to 694,547
_RS03140
RR42_RS03145: RR42_RS03145 - peptidase M48, at 694,642 to 696,450
_RS03145
RR42_RS03150: RR42_RS03150 - hypothetical protein, at 696,499 to 697,110
_RS03150
RR42_RS03155: RR42_RS03155 - alpha/beta hydrolase, at 697,188 to 698,174
_RS03155
Position (kb) 694
695
696
697 Strain fitness (log2 ratio) -2
-1
0
1 at 693.661 kb on - strand at 693.684 kb on + strand at 693.685 kb on - strand at 693.723 kb on - strand at 693.799 kb on + strand at 693.799 kb on + strand at 693.800 kb on - strand at 693.800 kb on - strand at 693.800 kb on - strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.936 kb on + strand at 693.937 kb on - strand at 693.965 kb on + strand at 694.028 kb on - strand at 694.066 kb on + strand at 694.066 kb on + strand at 694.067 kb on - strand at 694.168 kb on + strand, within RR42_RS03140 at 694.168 kb on + strand, within RR42_RS03140 at 694.169 kb on - strand, within RR42_RS03140 at 694.169 kb on - strand, within RR42_RS03140 at 694.169 kb on - strand, within RR42_RS03140 at 694.285 kb on + strand, within RR42_RS03140 at 694.324 kb on - strand, within RR42_RS03140 at 694.383 kb on + strand, within RR42_RS03140 at 694.383 kb on + strand, within RR42_RS03140 at 694.383 kb on + strand, within RR42_RS03140 at 694.505 kb on + strand at 694.505 kb on + strand at 694.505 kb on + strand at 694.506 kb on - strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.671 kb on + strand at 694.671 kb on + strand at 694.671 kb on + strand at 694.671 kb on + strand at 694.672 kb on - strand at 694.672 kb on - strand at 694.672 kb on - strand at 694.855 kb on - strand, within RR42_RS03145 at 694.855 kb on - strand, within RR42_RS03145 at 694.912 kb on + strand, within RR42_RS03145 at 694.912 kb on + strand, within RR42_RS03145 at 694.913 kb on - strand, within RR42_RS03145 at 694.913 kb on - strand, within RR42_RS03145 at 694.913 kb on - strand, within RR42_RS03145 at 695.104 kb on + strand, within RR42_RS03145 at 695.104 kb on + strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.131 kb on + strand, within RR42_RS03145 at 695.132 kb on - strand, within RR42_RS03145 at 695.279 kb on - strand, within RR42_RS03145 at 695.280 kb on + strand, within RR42_RS03145 at 695.281 kb on - strand, within RR42_RS03145 at 695.377 kb on - strand, within RR42_RS03145 at 695.377 kb on - strand, within RR42_RS03145 at 695.668 kb on + strand, within RR42_RS03145 at 695.669 kb on - strand, within RR42_RS03145 at 695.765 kb on - strand, within RR42_RS03145 at 695.968 kb on + strand, within RR42_RS03145 at 695.969 kb on - strand, within RR42_RS03145 at 695.969 kb on - strand, within RR42_RS03145 at 695.969 kb on - strand, within RR42_RS03145 at 696.121 kb on + strand, within RR42_RS03145 at 696.122 kb on - strand, within RR42_RS03145 at 696.122 kb on - strand, within RR42_RS03145 at 696.236 kb on - strand, within RR42_RS03145 at 696.236 kb on - strand, within RR42_RS03145 at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.287 kb on - strand at 696.287 kb on - strand at 696.305 kb on - strand at 696.361 kb on + strand at 696.362 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.614 kb on - strand, within RR42_RS03150 at 696.663 kb on + strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.905 kb on + strand, within RR42_RS03150 at 697.004 kb on + strand, within RR42_RS03150 at 697.005 kb on - strand, within RR42_RS03150 at 697.055 kb on + strand at 697.066 kb on + strand at 697.168 kb on - strand at 697.168 kb on - strand at 697.239 kb on - strand at 697.277 kb on + strand at 697.277 kb on + strand at 697.278 kb on - strand at 697.370 kb on - strand, within RR42_RS03155
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 693,661 - -0.8 693,684 + +0.5 693,685 - +0.5 693,723 - +0.0 693,799 + +1.1 693,799 + -0.6 693,800 - +0.0 693,800 - -1.7 693,800 - +0.3 693,933 + -1.0 693,933 + -0.6 693,933 + -0.2 693,933 + -0.1 693,933 + -1.5 693,933 + -0.6 693,933 + +0.5 693,934 - -0.9 693,934 - +1.1 693,934 - -2.1 693,934 - +0.5 693,934 - +0.6 693,936 + +0.5 693,937 - -0.0 693,965 + -0.5 694,028 - -0.0 694,066 + -0.2 694,066 + +0.4 694,067 - -1.4 694,168 + RR42_RS03140 0.21 +0.1 694,168 + RR42_RS03140 0.21 -0.0 694,169 - RR42_RS03140 0.22 -0.4 694,169 - RR42_RS03140 0.22 -0.2 694,169 - RR42_RS03140 0.22 +0.2 694,285 + RR42_RS03140 0.46 +0.3 694,324 - RR42_RS03140 0.54 -0.3 694,383 + RR42_RS03140 0.66 +0.4 694,383 + RR42_RS03140 0.66 +0.7 694,383 + RR42_RS03140 0.66 -0.7 694,505 + +0.4 694,505 + -0.4 694,505 + -0.6 694,506 - -0.3 694,667 - -0.2 694,667 - -1.6 694,667 - -0.4 694,671 + +0.0 694,671 + -0.6 694,671 + -0.1 694,671 + +0.8 694,672 - -0.5 694,672 - -0.3 694,672 - +0.0 694,855 - RR42_RS03145 0.12 +0.3 694,855 - RR42_RS03145 0.12 +0.6 694,912 + RR42_RS03145 0.15 -0.9 694,912 + RR42_RS03145 0.15 -0.5 694,913 - RR42_RS03145 0.15 +0.1 694,913 - RR42_RS03145 0.15 -1.3 694,913 - RR42_RS03145 0.15 -1.9 695,104 + RR42_RS03145 0.26 -1.0 695,104 + RR42_RS03145 0.26 -1.6 695,105 - RR42_RS03145 0.26 -0.4 695,105 - RR42_RS03145 0.26 +0.2 695,105 - RR42_RS03145 0.26 +0.2 695,105 - RR42_RS03145 0.26 -1.3 695,131 + RR42_RS03145 0.27 +0.3 695,132 - RR42_RS03145 0.27 +0.0 695,279 - RR42_RS03145 0.35 +0.4 695,280 + RR42_RS03145 0.35 -1.3 695,281 - RR42_RS03145 0.35 -0.4 695,377 - RR42_RS03145 0.41 +1.3 695,377 - RR42_RS03145 0.41 -0.4 695,668 + RR42_RS03145 0.57 -0.1 695,669 - RR42_RS03145 0.57 -0.1 695,765 - RR42_RS03145 0.62 +0.1 695,968 + RR42_RS03145 0.73 -0.7 695,969 - RR42_RS03145 0.73 -1.0 695,969 - RR42_RS03145 0.73 +0.1 695,969 - RR42_RS03145 0.73 -0.1 696,121 + RR42_RS03145 0.82 -1.4 696,122 - RR42_RS03145 0.82 -0.5 696,122 - RR42_RS03145 0.82 +0.2 696,236 - RR42_RS03145 0.88 -1.9 696,236 - RR42_RS03145 0.88 -1.8 696,286 + -0.5 696,286 + +0.4 696,286 + -0.6 696,286 + -0.5 696,286 + +0.2 696,287 - -1.4 696,287 - -0.1 696,305 - -0.9 696,361 + -1.6 696,362 - -1.6 696,445 - +0.5 696,445 - +0.3 696,445 - -0.1 696,614 - RR42_RS03150 0.19 +0.2 696,663 + RR42_RS03150 0.27 -0.2 696,664 - RR42_RS03150 0.27 -0.4 696,664 - RR42_RS03150 0.27 -0.0 696,864 - RR42_RS03150 0.60 -0.5 696,864 - RR42_RS03150 0.60 -0.3 696,905 + RR42_RS03150 0.66 +0.3 697,004 + RR42_RS03150 0.83 +0.2 697,005 - RR42_RS03150 0.83 -0.3 697,055 + -0.4 697,066 + +0.4 697,168 - +0.6 697,168 - +0.6 697,239 - +0.9 697,277 + +0.6 697,277 + -0.1 697,278 - -0.3 697,370 - RR42_RS03155 0.18 +0.7
Or see this region's nucleotide sequence