Experiment: R2A with Nickel (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01125 and RR42_RS01130 are separated by 55 nucleotides RR42_RS01130 and RR42_RS01135 overlap by 4 nucleotides RR42_RS01135 and RR42_RS01140 are separated by 50 nucleotides RR42_RS01140 and RR42_RS01145 are separated by 16 nucleotides
RR42_RS01125: RR42_RS01125 - acetylglutamate kinase, at 259,367 to 260,284
_RS01125
RR42_RS01130: RR42_RS01130 - haloacid dehalogenase, at 260,340 to 261,320
_RS01130
RR42_RS01135: RR42_RS01135 - dihydroorotate oxidase, at 261,317 to 261,976
_RS01135
RR42_RS01140: RR42_RS01140 - hypothetical protein, at 262,027 to 262,260
_RS01140
RR42_RS01145: RR42_RS01145 - membrane protein, at 262,277 to 263,710
_RS01145
Position (kb) 260
261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.282 kb on + strand at 260.293 kb on + strand at 260.294 kb on - strand at 260.294 kb on - strand at 260.349 kb on - strand at 260.349 kb on - strand at 260.349 kb on - strand at 260.505 kb on + strand, within RR42_RS01130 at 260.505 kb on + strand, within RR42_RS01130 at 260.506 kb on - strand, within RR42_RS01130 at 260.614 kb on + strand, within RR42_RS01130 at 260.615 kb on - strand, within RR42_RS01130 at 260.751 kb on + strand, within RR42_RS01130 at 260.986 kb on - strand, within RR42_RS01130 at 260.986 kb on - strand, within RR42_RS01130 at 261.265 kb on + strand at 261.265 kb on + strand at 261.265 kb on + strand at 261.272 kb on - strand at 261.974 kb on + strand at 261.975 kb on - strand at 262.028 kb on + strand at 262.250 kb on + strand at 262.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.25 mM remove 260,282 + +0.2 260,293 + +0.3 260,294 - -0.6 260,294 - -0.7 260,349 - -0.5 260,349 - -0.1 260,349 - +0.1 260,505 + RR42_RS01130 0.17 -0.2 260,505 + RR42_RS01130 0.17 +0.1 260,506 - RR42_RS01130 0.17 -0.4 260,614 + RR42_RS01130 0.28 +1.4 260,615 - RR42_RS01130 0.28 -1.2 260,751 + RR42_RS01130 0.42 -0.8 260,986 - RR42_RS01130 0.66 -0.3 260,986 - RR42_RS01130 0.66 -0.1 261,265 + +1.0 261,265 + -0.3 261,265 + +0.0 261,272 - +0.2 261,974 + -0.6 261,975 - -0.1 262,028 + -3.5 262,250 + -0.3 262,250 + -0.5
Or see this region's nucleotide sequence