Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_14345

Experiment: Minimal media pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_14335 and AO356_14340 overlap by 4 nucleotidesAO356_14340 and AO356_14345 overlap by 4 nucleotidesAO356_14345 and AO356_14350 are separated by 16 nucleotides AO356_14335: AO356_14335 - rRNA methyltransferase, at 3,259,240 to 3,260,058 _14335 AO356_14340: AO356_14340 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,260,055 to 3,261,044 _14340 AO356_14345: AO356_14345 - molecular chaperone SurA, at 3,261,041 to 3,262,321 _14345 AO356_14350: AO356_14350 - LPS biosynthesis protein, at 3,262,338 to 3,265,136 _14350 Position (kb) 3261 3262 3263Strain fitness (log2 ratio) -1 0 1at 3260.045 kb on + strandat 3260.046 kb on - strandat 3260.058 kb on + strandat 3260.058 kb on + strandat 3260.059 kb on - strandat 3262.399 kb on - strandat 3262.399 kb on - strandat 3262.399 kb on - strandat 3262.399 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH8
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3,260,045 + -0.8
3,260,046 - -0.9
3,260,058 + +0.3
3,260,058 + +0.1
3,260,059 - -0.6
3,262,399 - -0.3
3,262,399 - +0.9
3,262,399 - +0.4
3,262,399 - -0.2

Or see this region's nucleotide sequence