Experiment: Minimal media pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00335 and AO356_00340 are separated by 222 nucleotides AO356_00340 and AO356_00345 are separated by 63 nucleotides AO356_00345 and AO356_00350 are separated by 32 nucleotides
AO356_00335: AO356_00335 - integrase, at 65,556 to 67,397
_00335
AO356_00340: AO356_00340 - tRNA-Gly, at 67,620 to 67,695
_00340
AO356_00345: AO356_00345 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 67,759 to 68,319
_00345
AO356_00350: AO356_00350 - excinuclease ABC subunit C, at 68,352 to 70,175
_00350
Position (kb) 67
68 Strain fitness (log2 ratio) -1
0
1 at 66.719 kb on - strand, within AO356_00335 at 66.936 kb on + strand, within AO356_00335 at 66.936 kb on + strand, within AO356_00335 at 66.942 kb on + strand, within AO356_00335 at 66.943 kb on - strand, within AO356_00335 at 66.967 kb on + strand, within AO356_00335 at 66.967 kb on + strand, within AO356_00335 at 66.967 kb on + strand, within AO356_00335 at 66.967 kb on + strand, within AO356_00335 at 66.968 kb on - strand, within AO356_00335 at 66.971 kb on + strand, within AO356_00335 at 66.971 kb on + strand, within AO356_00335 at 66.971 kb on + strand, within AO356_00335 at 66.972 kb on - strand, within AO356_00335 at 66.972 kb on - strand, within AO356_00335 at 67.153 kb on + strand, within AO356_00335 at 67.153 kb on + strand, within AO356_00335 at 67.153 kb on + strand, within AO356_00335 at 67.181 kb on + strand, within AO356_00335 at 67.182 kb on - strand, within AO356_00335 at 67.231 kb on + strand at 67.231 kb on + strand at 67.274 kb on - strand at 67.345 kb on + strand at 67.345 kb on + strand at 67.346 kb on - strand at 67.506 kb on + strand at 67.507 kb on - strand at 67.519 kb on + strand at 67.564 kb on - strand at 67.717 kb on + strand at 67.718 kb on - strand at 68.354 kb on - strand at 68.375 kb on - strand at 68.375 kb on - strand at 68.375 kb on - strand at 68.397 kb on - strand at 68.397 kb on - strand at 68.418 kb on + strand at 68.418 kb on + strand at 68.419 kb on - strand at 68.519 kb on + strand at 68.520 kb on - strand at 68.525 kb on + strand at 68.526 kb on - strand at 68.659 kb on + strand, within AO356_00350 at 68.660 kb on - strand, within AO356_00350
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH8 remove 66,719 - AO356_00335 0.63 -1.0 66,936 + AO356_00335 0.75 -1.1 66,936 + AO356_00335 0.75 -0.7 66,942 + AO356_00335 0.75 +0.6 66,943 - AO356_00335 0.75 -0.1 66,967 + AO356_00335 0.77 +1.5 66,967 + AO356_00335 0.77 +1.3 66,967 + AO356_00335 0.77 -0.0 66,967 + AO356_00335 0.77 +0.1 66,968 - AO356_00335 0.77 -0.2 66,971 + AO356_00335 0.77 -0.2 66,971 + AO356_00335 0.77 +0.1 66,971 + AO356_00335 0.77 -1.7 66,972 - AO356_00335 0.77 -0.1 66,972 - AO356_00335 0.77 -0.5 67,153 + AO356_00335 0.87 -0.2 67,153 + AO356_00335 0.87 -0.5 67,153 + AO356_00335 0.87 +0.6 67,181 + AO356_00335 0.88 -0.6 67,182 - AO356_00335 0.88 -0.8 67,231 + -0.2 67,231 + +0.2 67,274 - +0.3 67,345 + +1.2 67,345 + -1.2 67,346 - -0.2 67,506 + +0.7 67,507 - +0.1 67,519 + -0.2 67,564 - -0.3 67,717 + -0.2 67,718 - +0.8 68,354 - +0.0 68,375 - +0.3 68,375 - +1.5 68,375 - +0.6 68,397 - +0.1 68,397 - -0.1 68,418 + +0.6 68,418 + +0.3 68,419 - -0.7 68,519 + +0.4 68,520 - +0.2 68,525 + -0.5 68,526 - -0.7 68,659 + AO356_00350 0.17 +0.5 68,660 - AO356_00350 0.17 +0.0
Or see this region's nucleotide sequence