Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27235 and BWI76_RS27240 are separated by 3 nucleotides BWI76_RS27240 and BWI76_RS27245 overlap by 4 nucleotides BWI76_RS27245 and BWI76_RS27250 are separated by 160 nucleotides
BWI76_RS27235: BWI76_RS27235 - murein hydrolase activator EnvC, at 5,652,316 to 5,653,587
_RS27235
BWI76_RS27240: BWI76_RS27240 - hypothetical protein, at 5,653,591 to 5,654,523
_RS27240
BWI76_RS27245: BWI76_RS27245 - glycosyl transferase family protein, at 5,654,520 to 5,655,317
_RS27245
BWI76_RS27250: BWI76_RS27250 - L-threonine 3-dehydrogenase, at 5,655,478 to 5,656,503
_RS27250
Position (kb)
5653
5654
5655 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 5652.782 kb on - strand, within BWI76_RS27235 at 5652.842 kb on + strand, within BWI76_RS27235 at 5652.929 kb on - strand, within BWI76_RS27235 at 5653.100 kb on + strand, within BWI76_RS27235 at 5653.122 kb on + strand, within BWI76_RS27235 at 5653.148 kb on + strand, within BWI76_RS27235 at 5653.204 kb on - strand, within BWI76_RS27235 at 5653.266 kb on + strand, within BWI76_RS27235 at 5653.272 kb on + strand, within BWI76_RS27235 at 5653.323 kb on + strand, within BWI76_RS27235 at 5653.454 kb on - strand, within BWI76_RS27235 at 5653.464 kb on + strand at 5653.507 kb on + strand at 5653.562 kb on + strand at 5653.570 kb on - strand at 5653.643 kb on - strand at 5653.763 kb on - strand, within BWI76_RS27240 at 5653.879 kb on + strand, within BWI76_RS27240 at 5653.900 kb on - strand, within BWI76_RS27240 at 5653.903 kb on - strand, within BWI76_RS27240 at 5653.948 kb on - strand, within BWI76_RS27240 at 5653.962 kb on + strand, within BWI76_RS27240 at 5653.965 kb on - strand, within BWI76_RS27240 at 5653.998 kb on - strand, within BWI76_RS27240 at 5654.067 kb on + strand, within BWI76_RS27240 at 5654.085 kb on + strand, within BWI76_RS27240 at 5654.088 kb on - strand, within BWI76_RS27240 at 5654.187 kb on + strand, within BWI76_RS27240 at 5654.195 kb on - strand, within BWI76_RS27240 at 5654.223 kb on + strand, within BWI76_RS27240 at 5654.254 kb on + strand, within BWI76_RS27240 at 5654.254 kb on + strand, within BWI76_RS27240 at 5654.278 kb on + strand, within BWI76_RS27240 at 5654.295 kb on - strand, within BWI76_RS27240 at 5654.332 kb on - strand, within BWI76_RS27240 at 5654.568 kb on + strand at 5654.571 kb on - strand at 5654.588 kb on - strand at 5654.621 kb on + strand, within BWI76_RS27245 at 5654.644 kb on + strand, within BWI76_RS27245 at 5654.673 kb on - strand, within BWI76_RS27245 at 5654.799 kb on + strand, within BWI76_RS27245 at 5654.805 kb on - strand, within BWI76_RS27245 at 5654.807 kb on - strand, within BWI76_RS27245 at 5655.008 kb on - strand, within BWI76_RS27245 at 5655.024 kb on - strand, within BWI76_RS27245 at 5655.038 kb on - strand, within BWI76_RS27245 at 5655.065 kb on + strand, within BWI76_RS27245 at 5655.075 kb on + strand, within BWI76_RS27245 at 5655.094 kb on - strand, within BWI76_RS27245 at 5655.094 kb on - strand, within BWI76_RS27245 at 5655.142 kb on + strand, within BWI76_RS27245 at 5655.258 kb on - strand at 5655.329 kb on + strand at 5655.330 kb on + strand at 5655.339 kb on + strand at 5655.347 kb on - strand at 5655.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 5,652,782 - BWI76_RS27235 0.37 +1.3 5,652,842 + BWI76_RS27235 0.41 +1.4 5,652,929 - BWI76_RS27235 0.48 +1.2 5,653,100 + BWI76_RS27235 0.62 +0.9 5,653,122 + BWI76_RS27235 0.63 +0.4 5,653,148 + BWI76_RS27235 0.65 +2.8 5,653,204 - BWI76_RS27235 0.70 +0.9 5,653,266 + BWI76_RS27235 0.75 +1.3 5,653,272 + BWI76_RS27235 0.75 +1.5 5,653,323 + BWI76_RS27235 0.79 +2.0 5,653,454 - BWI76_RS27235 0.89 +0.9 5,653,464 + +0.7 5,653,507 + +1.6 5,653,562 + -0.4 5,653,570 - +1.1 5,653,643 - +1.6 5,653,763 - BWI76_RS27240 0.18 +0.2 5,653,879 + BWI76_RS27240 0.31 +0.1 5,653,900 - BWI76_RS27240 0.33 +0.8 5,653,903 - BWI76_RS27240 0.33 +0.3 5,653,948 - BWI76_RS27240 0.38 +0.7 5,653,962 + BWI76_RS27240 0.40 +1.0 5,653,965 - BWI76_RS27240 0.40 +3.1 5,653,998 - BWI76_RS27240 0.44 +1.3 5,654,067 + BWI76_RS27240 0.51 +0.8 5,654,085 + BWI76_RS27240 0.53 +1.0 5,654,088 - BWI76_RS27240 0.53 +0.8 5,654,187 + BWI76_RS27240 0.64 +0.9 5,654,195 - BWI76_RS27240 0.65 +0.3 5,654,223 + BWI76_RS27240 0.68 +0.1 5,654,254 + BWI76_RS27240 0.71 +0.3 5,654,254 + BWI76_RS27240 0.71 +0.5 5,654,278 + BWI76_RS27240 0.74 +1.5 5,654,295 - BWI76_RS27240 0.75 +0.3 5,654,332 - BWI76_RS27240 0.79 -0.6 5,654,568 + +3.8 5,654,571 - -0.2 5,654,588 - -0.5 5,654,621 + BWI76_RS27245 0.13 +0.6 5,654,644 + BWI76_RS27245 0.16 +1.8 5,654,673 - BWI76_RS27245 0.19 -0.2 5,654,799 + BWI76_RS27245 0.35 -0.4 5,654,805 - BWI76_RS27245 0.36 +0.1 5,654,807 - BWI76_RS27245 0.36 +0.1 5,655,008 - BWI76_RS27245 0.61 -0.5 5,655,024 - BWI76_RS27245 0.63 +0.3 5,655,038 - BWI76_RS27245 0.65 +0.1 5,655,065 + BWI76_RS27245 0.68 +0.3 5,655,075 + BWI76_RS27245 0.70 +0.7 5,655,094 - BWI76_RS27245 0.72 -0.3 5,655,094 - BWI76_RS27245 0.72 -0.5 5,655,142 + BWI76_RS27245 0.78 +0.1 5,655,258 - +0.4 5,655,329 + +1.0 5,655,330 + -0.2 5,655,339 + +0.1 5,655,347 - -0.1 5,655,510 + +0.2
Or see this region's nucleotide sequence