Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22335 and BWI76_RS22340 are separated by 82 nucleotides BWI76_RS22340 and BWI76_RS22345 are separated by 208 nucleotides BWI76_RS22345 and BWI76_RS22350 are separated by 3 nucleotides BWI76_RS22350 and BWI76_RS22355 overlap by 10 nucleotides
BWI76_RS22335: BWI76_RS22335 - 4Fe-4S ferredoxin, at 4,656,627 to 4,657,235
_RS22335
BWI76_RS22340: BWI76_RS22340 - formate hydrogenlyase regulatory protein HycA, at 4,657,318 to 4,657,767
_RS22340
BWI76_RS22345: BWI76_RS22345 - hydrogenase maturation nickel metallochaperone HypA, at 4,657,976 to 4,658,326
_RS22345
BWI76_RS22350: BWI76_RS22350 - hydrogenase nickel incorporation protein HypB, at 4,658,330 to 4,659,202
_RS22350
BWI76_RS22355: BWI76_RS22355 - hydrogenase assembly chaperone, at 4,659,193 to 4,659,465
_RS22355
Position (kb) 4657
4658
4659 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4657.292 kb on - strand at 4657.609 kb on - strand, within BWI76_RS22340 at 4657.659 kb on + strand, within BWI76_RS22340 at 4657.659 kb on + strand, within BWI76_RS22340 at 4657.667 kb on - strand, within BWI76_RS22340 at 4657.671 kb on + strand, within BWI76_RS22340 at 4657.783 kb on + strand at 4657.783 kb on + strand at 4657.989 kb on + strand at 4657.989 kb on + strand at 4657.997 kb on - strand at 4658.045 kb on + strand, within BWI76_RS22345 at 4658.102 kb on + strand, within BWI76_RS22345 at 4658.151 kb on + strand, within BWI76_RS22345 at 4658.161 kb on + strand, within BWI76_RS22345 at 4658.174 kb on - strand, within BWI76_RS22345 at 4658.259 kb on - strand, within BWI76_RS22345 at 4658.426 kb on + strand, within BWI76_RS22350 at 4658.470 kb on + strand, within BWI76_RS22350 at 4658.478 kb on - strand, within BWI76_RS22350 at 4658.745 kb on - strand, within BWI76_RS22350 at 4658.745 kb on - strand, within BWI76_RS22350 at 4658.950 kb on + strand, within BWI76_RS22350 at 4659.156 kb on + strand at 4659.163 kb on - strand at 4659.254 kb on - strand, within BWI76_RS22355
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 4,657,292 - +0.9 4,657,609 - BWI76_RS22340 0.65 -0.1 4,657,659 + BWI76_RS22340 0.76 -0.2 4,657,659 + BWI76_RS22340 0.76 -0.1 4,657,667 - BWI76_RS22340 0.78 -0.1 4,657,671 + BWI76_RS22340 0.78 -0.5 4,657,783 + -0.1 4,657,783 + +0.4 4,657,989 + +1.0 4,657,989 + -0.3 4,657,997 - -0.4 4,658,045 + BWI76_RS22345 0.20 -1.8 4,658,102 + BWI76_RS22345 0.36 +1.7 4,658,151 + BWI76_RS22345 0.50 -0.1 4,658,161 + BWI76_RS22345 0.53 +1.2 4,658,174 - BWI76_RS22345 0.56 +0.1 4,658,259 - BWI76_RS22345 0.81 -0.3 4,658,426 + BWI76_RS22350 0.11 -0.5 4,658,470 + BWI76_RS22350 0.16 +4.0 4,658,478 - BWI76_RS22350 0.17 -0.3 4,658,745 - BWI76_RS22350 0.48 +4.1 4,658,745 - BWI76_RS22350 0.48 -0.4 4,658,950 + BWI76_RS22350 0.71 +0.6 4,659,156 + -0.7 4,659,163 - +3.9 4,659,254 - BWI76_RS22355 0.22 +0.8
Or see this region's nucleotide sequence