Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22125 and BWI76_RS22130 overlap by 4 nucleotides BWI76_RS22130 and BWI76_RS22135 are separated by 127 nucleotides BWI76_RS22135 and BWI76_RS22140 are separated by 203 nucleotides BWI76_RS22140 and BWI76_RS22145 are separated by 192 nucleotides BWI76_RS22145 and BWI76_RS22150 are separated by 196 nucleotides BWI76_RS22150 and BWI76_RS22155 are separated by 60 nucleotides BWI76_RS22155 and BWI76_RS22160 are separated by 4 nucleotides
BWI76_RS22125: BWI76_RS22125 - membrane protein, at 4,619,468 to 4,619,896
_RS22125
BWI76_RS22130: BWI76_RS22130 - fructose-1-phosphate/6-phosphogluconate phosphatase, at 4,619,893 to 4,620,459
_RS22130
BWI76_RS22135: BWI76_RS22135 - transport-associated protein, at 4,620,587 to 4,620,901
_RS22135
BWI76_RS22140: BWI76_RS22140 - tRNA-Arg, at 4,621,105 to 4,621,181
_RS22140
BWI76_RS22145: BWI76_RS22145 - tRNA-Arg, at 4,621,374 to 4,621,450
_RS22145
BWI76_RS22150: BWI76_RS22150 - tRNA-Arg, at 4,621,647 to 4,621,723
_RS22150
BWI76_RS22155: BWI76_RS22155 - tRNA-Arg, at 4,621,784 to 4,621,860
_RS22155
BWI76_RS22160: BWI76_RS22160 - tRNA-Ser, at 4,621,865 to 4,621,957
_RS22160
Position (kb)
4620
4621 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4619.594 kb on - strand, within BWI76_RS22125 at 4619.772 kb on + strand, within BWI76_RS22125 at 4619.780 kb on - strand, within BWI76_RS22125 at 4619.847 kb on - strand, within BWI76_RS22125 at 4619.881 kb on + strand at 4619.941 kb on + strand at 4620.018 kb on - strand, within BWI76_RS22130 at 4620.032 kb on + strand, within BWI76_RS22130 at 4620.184 kb on - strand, within BWI76_RS22130 at 4620.188 kb on + strand, within BWI76_RS22130 at 4620.613 kb on - strand at 4620.718 kb on - strand, within BWI76_RS22135 at 4620.762 kb on + strand, within BWI76_RS22135 at 4620.796 kb on - strand, within BWI76_RS22135 at 4620.912 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 4,619,594 - BWI76_RS22125 0.29 +0.8 4,619,772 + BWI76_RS22125 0.71 +0.5 4,619,780 - BWI76_RS22125 0.73 -0.1 4,619,847 - BWI76_RS22125 0.88 -0.4 4,619,881 + -2.9 4,619,941 + -0.1 4,620,018 - BWI76_RS22130 0.22 -0.8 4,620,032 + BWI76_RS22130 0.25 -0.3 4,620,184 - BWI76_RS22130 0.51 -0.5 4,620,188 + BWI76_RS22130 0.52 +0.0 4,620,613 - -0.2 4,620,718 - BWI76_RS22135 0.42 -0.9 4,620,762 + BWI76_RS22135 0.56 -0.9 4,620,796 - BWI76_RS22135 0.66 -1.4 4,620,912 + +1.4
Or see this region's nucleotide sequence