Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22135

Experiment: Chloramphenicol 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22125 and BWI76_RS22130 overlap by 4 nucleotidesBWI76_RS22130 and BWI76_RS22135 are separated by 127 nucleotidesBWI76_RS22135 and BWI76_RS22140 are separated by 203 nucleotidesBWI76_RS22140 and BWI76_RS22145 are separated by 192 nucleotidesBWI76_RS22145 and BWI76_RS22150 are separated by 196 nucleotidesBWI76_RS22150 and BWI76_RS22155 are separated by 60 nucleotidesBWI76_RS22155 and BWI76_RS22160 are separated by 4 nucleotides BWI76_RS22125: BWI76_RS22125 - membrane protein, at 4,619,468 to 4,619,896 _RS22125 BWI76_RS22130: BWI76_RS22130 - fructose-1-phosphate/6-phosphogluconate phosphatase, at 4,619,893 to 4,620,459 _RS22130 BWI76_RS22135: BWI76_RS22135 - transport-associated protein, at 4,620,587 to 4,620,901 _RS22135 BWI76_RS22140: BWI76_RS22140 - tRNA-Arg, at 4,621,105 to 4,621,181 _RS22140 BWI76_RS22145: BWI76_RS22145 - tRNA-Arg, at 4,621,374 to 4,621,450 _RS22145 BWI76_RS22150: BWI76_RS22150 - tRNA-Arg, at 4,621,647 to 4,621,723 _RS22150 BWI76_RS22155: BWI76_RS22155 - tRNA-Arg, at 4,621,784 to 4,621,860 _RS22155 BWI76_RS22160: BWI76_RS22160 - tRNA-Ser, at 4,621,865 to 4,621,957 _RS22160 Position (kb) 4620 4621Strain fitness (log2 ratio) -3 -2 -1 0 1at 4619.594 kb on - strand, within BWI76_RS22125at 4619.772 kb on + strand, within BWI76_RS22125at 4619.780 kb on - strand, within BWI76_RS22125at 4619.847 kb on - strand, within BWI76_RS22125at 4619.881 kb on + strandat 4619.941 kb on + strandat 4620.018 kb on - strand, within BWI76_RS22130at 4620.032 kb on + strand, within BWI76_RS22130at 4620.184 kb on - strand, within BWI76_RS22130at 4620.188 kb on + strand, within BWI76_RS22130at 4620.613 kb on - strandat 4620.718 kb on - strand, within BWI76_RS22135at 4620.762 kb on + strand, within BWI76_RS22135at 4620.796 kb on - strand, within BWI76_RS22135at 4620.912 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml
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4,619,594 - BWI76_RS22125 0.29 +0.8
4,619,772 + BWI76_RS22125 0.71 +0.5
4,619,780 - BWI76_RS22125 0.73 -0.1
4,619,847 - BWI76_RS22125 0.88 -0.4
4,619,881 + -2.9
4,619,941 + -0.1
4,620,018 - BWI76_RS22130 0.22 -0.8
4,620,032 + BWI76_RS22130 0.25 -0.3
4,620,184 - BWI76_RS22130 0.51 -0.5
4,620,188 + BWI76_RS22130 0.52 +0.0
4,620,613 - -0.2
4,620,718 - BWI76_RS22135 0.42 -0.9
4,620,762 + BWI76_RS22135 0.56 -0.9
4,620,796 - BWI76_RS22135 0.66 -1.4
4,620,912 + +1.4

Or see this region's nucleotide sequence