Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20670 and BWI76_RS20675 are separated by 14 nucleotides BWI76_RS20675 and BWI76_RS20680 are separated by 45 nucleotides BWI76_RS20680 and BWI76_RS20685 overlap by 4 nucleotides
BWI76_RS20670: BWI76_RS20670 - transcriptional regulator PtsJ, at 4,324,370 to 4,325,668
_RS20670
BWI76_RS20675: BWI76_RS20675 - GMP synthase, at 4,325,683 to 4,326,390
_RS20675
BWI76_RS20680: BWI76_RS20680 - hypothetical protein, at 4,326,436 to 4,326,840
_RS20680
BWI76_RS20685: BWI76_RS20685 - cysteine synthase B, at 4,326,837 to 4,327,748
_RS20685
Position (kb)
4325
4326
4327 Strain fitness (log2 ratio)
-1
0
1 at 4325.054 kb on - strand, within BWI76_RS20670 at 4325.140 kb on + strand, within BWI76_RS20670 at 4325.549 kb on + strand at 4325.701 kb on - strand at 4325.726 kb on + strand at 4325.731 kb on + strand at 4325.779 kb on - strand, within BWI76_RS20675 at 4325.864 kb on + strand, within BWI76_RS20675 at 4325.904 kb on + strand, within BWI76_RS20675 at 4326.249 kb on + strand, within BWI76_RS20675 at 4326.249 kb on + strand at 4326.258 kb on - strand, within BWI76_RS20675 at 4326.315 kb on + strand, within BWI76_RS20675 at 4327.165 kb on - strand, within BWI76_RS20685
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 4,325,054 - BWI76_RS20670 0.53 +1.0 4,325,140 + BWI76_RS20670 0.59 -0.7 4,325,549 + -0.1 4,325,701 - -0.4 4,325,726 + -0.5 4,325,731 + -1.2 4,325,779 - BWI76_RS20675 0.14 -0.5 4,325,864 + BWI76_RS20675 0.26 -0.7 4,325,904 + BWI76_RS20675 0.31 -0.6 4,326,249 + BWI76_RS20675 0.80 -0.8 4,326,249 + +1.3 4,326,258 - BWI76_RS20675 0.81 -1.1 4,326,315 + BWI76_RS20675 0.89 +0.0 4,327,165 - BWI76_RS20685 0.36 +0.1
Or see this region's nucleotide sequence