Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17800 and BWI76_RS17805 are separated by 175 nucleotides BWI76_RS17805 and BWI76_RS17810 are separated by 43 nucleotides BWI76_RS17810 and BWI76_RS17815 are separated by 67 nucleotides
BWI76_RS17800: BWI76_RS17800 - long-chain-fatty-acid--CoA ligase, at 3,699,129 to 3,700,847
_RS17800
BWI76_RS17805: BWI76_RS17805 - membrane protein, at 3,701,023 to 3,701,607
_RS17805
BWI76_RS17810: BWI76_RS17810 - tRNA N6-adenosine(37)-N6-threonylcarbamoyltransferase complex dimerization subunit TsaB, at 3,701,651 to 3,702,346
_RS17810
BWI76_RS17815: BWI76_RS17815 - ATP-dependent helicase, at 3,702,414 to 3,704,324
_RS17815
Position (kb) 3701
3702
3703 Strain fitness (log2 ratio) -1
0
1
2
3 at 3700.716 kb on + strand at 3701.461 kb on - strand, within BWI76_RS17805 at 3702.456 kb on + strand at 3702.587 kb on - strand at 3702.590 kb on + strand at 3702.598 kb on - strand at 3702.866 kb on - strand, within BWI76_RS17815 at 3702.902 kb on - strand, within BWI76_RS17815 at 3702.917 kb on + strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.948 kb on - strand, within BWI76_RS17815 at 3702.990 kb on - strand, within BWI76_RS17815 at 3703.026 kb on - strand, within BWI76_RS17815 at 3703.037 kb on - strand, within BWI76_RS17815 at 3703.093 kb on - strand, within BWI76_RS17815
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 3,700,716 + -1.3 3,701,461 - BWI76_RS17805 0.75 +3.1 3,702,456 + -0.6 3,702,587 - +0.0 3,702,590 + +0.2 3,702,598 - +1.8 3,702,866 - BWI76_RS17815 0.24 +1.3 3,702,902 - BWI76_RS17815 0.26 -0.2 3,702,917 + BWI76_RS17815 0.26 -0.7 3,702,925 - BWI76_RS17815 0.27 -0.9 3,702,925 - BWI76_RS17815 0.27 +1.0 3,702,925 - BWI76_RS17815 0.27 +0.4 3,702,925 - BWI76_RS17815 0.27 -0.4 3,702,948 - BWI76_RS17815 0.28 +0.2 3,702,990 - BWI76_RS17815 0.30 +2.8 3,703,026 - BWI76_RS17815 0.32 +0.0 3,703,037 - BWI76_RS17815 0.33 -0.1 3,703,093 - BWI76_RS17815 0.36 -1.0
Or see this region's nucleotide sequence