Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16205 and BWI76_RS16210 are separated by 343 nucleotides BWI76_RS16210 and BWI76_RS16215 overlap by 4 nucleotides BWI76_RS16215 and BWI76_RS16220 overlap by 23 nucleotides BWI76_RS16220 and BWI76_RS16225 overlap by 1 nucleotides
BWI76_RS16205: BWI76_RS16205 - NAD(P) transhydrogenase subunit alpha, at 3,362,286 to 3,363,815
_RS16205
BWI76_RS16210: BWI76_RS16210 - hypothetical protein, at 3,364,159 to 3,364,341
_RS16210
BWI76_RS16215: BWI76_RS16215 - hypothetical protein, at 3,364,338 to 3,365,288
_RS16215
BWI76_RS16220: BWI76_RS16220 - hypothetical protein, at 3,365,266 to 3,365,468
_RS16220
BWI76_RS16225: BWI76_RS16225 - arginine:ornithine antiporter, at 3,365,468 to 3,366,850
_RS16225
Position (kb)
3364
3365
3366 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3363.786 kb on - strand at 3363.893 kb on + strand at 3364.072 kb on + strand at 3364.250 kb on + strand, within BWI76_RS16210 at 3364.318 kb on - strand, within BWI76_RS16210 at 3364.356 kb on + strand at 3364.364 kb on - strand at 3364.458 kb on + strand, within BWI76_RS16215 at 3364.581 kb on - strand, within BWI76_RS16215 at 3364.813 kb on + strand, within BWI76_RS16215 at 3364.835 kb on + strand, within BWI76_RS16215 at 3365.240 kb on + strand at 3365.251 kb on - strand at 3365.402 kb on - strand, within BWI76_RS16220 at 3365.522 kb on - strand at 3365.686 kb on - strand at 3365.958 kb on - strand, within BWI76_RS16225 at 3366.062 kb on - strand, within BWI76_RS16225 at 3366.087 kb on - strand, within BWI76_RS16225 at 3366.160 kb on - strand, within BWI76_RS16225
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 3,363,786 - +1.5 3,363,893 + +2.8 3,364,072 + +1.9 3,364,250 + BWI76_RS16210 0.50 -0.5 3,364,318 - BWI76_RS16210 0.87 -0.5 3,364,356 + +0.6 3,364,364 - +0.5 3,364,458 + BWI76_RS16215 0.13 -1.4 3,364,581 - BWI76_RS16215 0.26 -1.5 3,364,813 + BWI76_RS16215 0.50 -0.6 3,364,835 + BWI76_RS16215 0.52 -0.8 3,365,240 + -1.3 3,365,251 - -0.5 3,365,402 - BWI76_RS16220 0.67 -0.8 3,365,522 - -0.9 3,365,686 - -0.2 3,365,958 - BWI76_RS16225 0.35 +1.4 3,366,062 - BWI76_RS16225 0.43 -0.2 3,366,087 - BWI76_RS16225 0.45 +0.3 3,366,160 - BWI76_RS16225 0.50 -1.9
Or see this region's nucleotide sequence