Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15040 and BWI76_RS15045 are separated by 92 nucleotides BWI76_RS15045 and BWI76_RS15050 overlap by 18 nucleotides BWI76_RS15050 and BWI76_RS15055 are separated by 194 nucleotides
BWI76_RS15040: BWI76_RS15040 - putative 2-nitropropane dioxygenase, at 3,147,935 to 3,148,981
_RS15040
BWI76_RS15045: BWI76_RS15045 - LysR family transcriptional regulator, at 3,149,074 to 3,149,910
_RS15045
BWI76_RS15050: BWI76_RS15050 - sugar transporter, at 3,149,893 to 3,151,080
_RS15050
BWI76_RS15055: BWI76_RS15055 - lysine transporter LysE, at 3,151,275 to 3,151,895
_RS15055
Position (kb)
3149
3150
3151
3152 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3149.322 kb on - strand, within BWI76_RS15045 at 3149.332 kb on - strand, within BWI76_RS15045 at 3149.625 kb on + strand, within BWI76_RS15045 at 3149.848 kb on - strand at 3149.989 kb on - strand at 3150.125 kb on - strand, within BWI76_RS15050 at 3150.239 kb on - strand, within BWI76_RS15050 at 3150.461 kb on + strand, within BWI76_RS15050 at 3151.182 kb on - strand at 3151.390 kb on + strand, within BWI76_RS15055 at 3151.462 kb on + strand, within BWI76_RS15055 at 3151.577 kb on - strand, within BWI76_RS15055 at 3151.596 kb on + strand, within BWI76_RS15055 at 3151.604 kb on - strand, within BWI76_RS15055 at 3151.627 kb on - strand, within BWI76_RS15055 at 3151.662 kb on - strand, within BWI76_RS15055 at 3151.671 kb on - strand, within BWI76_RS15055 at 3151.673 kb on + strand, within BWI76_RS15055 at 3151.680 kb on + strand, within BWI76_RS15055 at 3151.680 kb on + strand, within BWI76_RS15055 at 3151.681 kb on - strand, within BWI76_RS15055 at 3151.682 kb on - strand, within BWI76_RS15055 at 3151.682 kb on - strand, within BWI76_RS15055 at 3151.688 kb on - strand, within BWI76_RS15055 at 3151.688 kb on - strand, within BWI76_RS15055 at 3151.701 kb on - strand, within BWI76_RS15055 at 3151.708 kb on - strand, within BWI76_RS15055 at 3151.708 kb on - strand, within BWI76_RS15055 at 3151.712 kb on + strand, within BWI76_RS15055 at 3151.727 kb on - strand, within BWI76_RS15055 at 3151.746 kb on + strand at 3151.761 kb on - strand, within BWI76_RS15055 at 3151.822 kb on + strand, within BWI76_RS15055 at 3151.843 kb on - strand at 3151.848 kb on - strand at 3151.871 kb on - strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.959 kb on - strand at 3151.959 kb on - strand at 3151.959 kb on - strand at 3151.990 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 3,149,322 - BWI76_RS15045 0.30 +0.3 3,149,332 - BWI76_RS15045 0.31 +0.1 3,149,625 + BWI76_RS15045 0.66 -0.2 3,149,848 - +1.9 3,149,989 - -0.9 3,150,125 - BWI76_RS15050 0.20 +3.4 3,150,239 - BWI76_RS15050 0.29 -0.3 3,150,461 + BWI76_RS15050 0.48 +0.3 3,151,182 - +0.9 3,151,390 + BWI76_RS15055 0.19 +3.5 3,151,462 + BWI76_RS15055 0.30 -0.5 3,151,577 - BWI76_RS15055 0.49 -0.2 3,151,596 + BWI76_RS15055 0.52 +1.2 3,151,604 - BWI76_RS15055 0.53 +0.0 3,151,627 - BWI76_RS15055 0.57 +0.2 3,151,662 - BWI76_RS15055 0.62 -1.6 3,151,671 - BWI76_RS15055 0.64 -0.1 3,151,673 + BWI76_RS15055 0.64 -1.5 3,151,680 + BWI76_RS15055 0.65 -1.5 3,151,680 + BWI76_RS15055 0.65 +0.0 3,151,681 - BWI76_RS15055 0.65 +0.4 3,151,682 - BWI76_RS15055 0.66 +0.3 3,151,682 - BWI76_RS15055 0.66 +0.3 3,151,688 - BWI76_RS15055 0.67 -0.2 3,151,688 - BWI76_RS15055 0.67 -0.2 3,151,701 - BWI76_RS15055 0.69 -0.5 3,151,708 - BWI76_RS15055 0.70 +0.1 3,151,708 - BWI76_RS15055 0.70 -0.4 3,151,712 + BWI76_RS15055 0.70 -2.2 3,151,727 - BWI76_RS15055 0.73 -0.7 3,151,746 + -0.0 3,151,761 - BWI76_RS15055 0.78 -0.1 3,151,822 + BWI76_RS15055 0.88 +2.8 3,151,843 - -1.1 3,151,848 - -0.2 3,151,871 - -0.4 3,151,951 + +0.9 3,151,951 + +2.5 3,151,951 + +0.6 3,151,951 + -0.3 3,151,951 + -0.5 3,151,959 - -1.7 3,151,959 - -0.6 3,151,959 - +1.4 3,151,990 - -0.1
Or see this region's nucleotide sequence