Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14835 and BWI76_RS14840 are separated by 180 nucleotides BWI76_RS14840 and BWI76_RS14845 are separated by 208 nucleotides BWI76_RS14845 and BWI76_RS14850 are separated by 366 nucleotides
BWI76_RS14835: BWI76_RS14835 - hypothetical protein, at 3,108,038 to 3,108,430
_RS14835
BWI76_RS14840: BWI76_RS14840 - hypothetical protein, at 3,108,611 to 3,109,213
_RS14840
BWI76_RS14845: BWI76_RS14845 - hypothetical protein, at 3,109,422 to 3,110,048
_RS14845
BWI76_RS14850: BWI76_RS14850 - LacI family transcriptional regulator, at 3,110,415 to 3,111,446
_RS14850
Position (kb)
3109
3110
3111 Strain fitness (log2 ratio)
-1
0
1
2 at 3108.425 kb on + strand at 3108.594 kb on - strand at 3108.726 kb on - strand, within BWI76_RS14840 at 3108.847 kb on - strand, within BWI76_RS14840 at 3109.135 kb on + strand, within BWI76_RS14840 at 3109.175 kb on - strand at 3109.240 kb on + strand at 3109.361 kb on - strand at 3109.674 kb on - strand, within BWI76_RS14845 at 3109.679 kb on - strand, within BWI76_RS14845 at 3109.803 kb on - strand, within BWI76_RS14845 at 3110.173 kb on - strand at 3110.219 kb on + strand at 3110.219 kb on + strand at 3110.219 kb on + strand at 3110.826 kb on - strand, within BWI76_RS14850 at 3110.991 kb on - strand, within BWI76_RS14850 at 3111.013 kb on - strand, within BWI76_RS14850
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 3,108,425 + +1.4 3,108,594 - +2.6 3,108,726 - BWI76_RS14840 0.19 -1.5 3,108,847 - BWI76_RS14840 0.39 +0.1 3,109,135 + BWI76_RS14840 0.87 -0.2 3,109,175 - -0.1 3,109,240 + +1.2 3,109,361 - -1.2 3,109,674 - BWI76_RS14845 0.40 +2.0 3,109,679 - BWI76_RS14845 0.41 +1.6 3,109,803 - BWI76_RS14845 0.61 -0.8 3,110,173 - +1.9 3,110,219 + -1.1 3,110,219 + +0.8 3,110,219 + -0.0 3,110,826 - BWI76_RS14850 0.40 -0.2 3,110,991 - BWI76_RS14850 0.56 +1.0 3,111,013 - BWI76_RS14850 0.58 +0.0
Or see this region's nucleotide sequence