Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10975 and BWI76_RS10980 are separated by 124 nucleotides BWI76_RS10980 and BWI76_RS10985 are separated by 285 nucleotides BWI76_RS10985 and BWI76_RS10990 are separated by 72 nucleotides
BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347
_RS10975
BWI76_RS10980: BWI76_RS10980 - biofilm formation regulatory protein BssS, at 2,317,472 to 2,317,726
_RS10980
BWI76_RS10985: BWI76_RS10985 - DNA damage-inducible protein I, at 2,318,012 to 2,318,257
_RS10985
BWI76_RS10990: BWI76_RS10990 - dihydroorotase, at 2,318,330 to 2,319,376
_RS10990
Position (kb)
2317
2318 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2316.539 kb on - strand, within BWI76_RS10975 at 2316.610 kb on - strand, within BWI76_RS10975 at 2317.096 kb on + strand, within BWI76_RS10975 at 2317.133 kb on + strand, within BWI76_RS10975 at 2317.198 kb on + strand, within BWI76_RS10975 at 2317.267 kb on + strand at 2317.274 kb on - strand at 2317.284 kb on + strand at 2317.284 kb on + strand at 2317.294 kb on + strand at 2317.337 kb on - strand at 2317.344 kb on + strand at 2317.352 kb on + strand at 2317.361 kb on - strand at 2317.367 kb on - strand at 2317.433 kb on - strand at 2317.492 kb on - strand at 2317.497 kb on - strand at 2317.517 kb on - strand, within BWI76_RS10980 at 2317.518 kb on - strand, within BWI76_RS10980 at 2317.638 kb on + strand, within BWI76_RS10980 at 2317.643 kb on + strand, within BWI76_RS10980 at 2317.670 kb on + strand, within BWI76_RS10980 at 2317.676 kb on - strand, within BWI76_RS10980 at 2317.680 kb on + strand, within BWI76_RS10980 at 2317.690 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.698 kb on - strand, within BWI76_RS10980 at 2317.705 kb on - strand at 2317.705 kb on - strand at 2317.726 kb on + strand at 2317.927 kb on + strand at 2317.935 kb on - strand at 2317.945 kb on - strand at 2318.025 kb on + strand at 2318.033 kb on - strand at 2318.033 kb on - strand at 2318.035 kb on - strand at 2318.045 kb on - strand, within BWI76_RS10985 at 2318.134 kb on + strand, within BWI76_RS10985 at 2318.152 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.439 kb on + strand, within BWI76_RS10990 at 2318.567 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 2,316,539 - BWI76_RS10975 0.28 +2.9 2,316,610 - BWI76_RS10975 0.34 -0.2 2,317,096 + BWI76_RS10975 0.77 -0.7 2,317,133 + BWI76_RS10975 0.81 -0.4 2,317,198 + BWI76_RS10975 0.87 -0.1 2,317,267 + +3.2 2,317,274 - +2.3 2,317,284 + -0.8 2,317,284 + +0.2 2,317,294 + +1.0 2,317,337 - +1.8 2,317,344 + -0.2 2,317,352 + -0.7 2,317,361 - -1.3 2,317,367 - +0.9 2,317,433 - -0.1 2,317,492 - -0.4 2,317,497 - -0.4 2,317,517 - BWI76_RS10980 0.18 -1.7 2,317,518 - BWI76_RS10980 0.18 -0.4 2,317,638 + BWI76_RS10980 0.65 +1.2 2,317,643 + BWI76_RS10980 0.67 +0.3 2,317,670 + BWI76_RS10980 0.78 +0.2 2,317,676 - BWI76_RS10980 0.80 +0.4 2,317,680 + BWI76_RS10980 0.82 -0.2 2,317,690 + BWI76_RS10980 0.85 -0.5 2,317,697 + BWI76_RS10980 0.88 +0.2 2,317,697 + BWI76_RS10980 0.88 -0.7 2,317,698 - BWI76_RS10980 0.89 -0.0 2,317,705 - -0.2 2,317,705 - -0.8 2,317,726 + -0.4 2,317,927 + +0.2 2,317,935 - +0.3 2,317,945 - +1.0 2,318,025 + -1.1 2,318,033 - +1.2 2,318,033 - -0.0 2,318,035 - +0.8 2,318,045 - BWI76_RS10985 0.13 +0.1 2,318,134 + BWI76_RS10985 0.50 -0.7 2,318,152 - BWI76_RS10985 0.57 -0.5 2,318,193 - BWI76_RS10985 0.74 -0.2 2,318,193 - BWI76_RS10985 0.74 -0.1 2,318,439 + BWI76_RS10990 0.10 -0.8 2,318,567 + +0.4
Or see this region's nucleotide sequence