Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotides BWI76_RS10055 and BWI76_RS10060 are separated by 51 nucleotides BWI76_RS10060 and BWI76_RS10065 overlap by 4 nucleotides BWI76_RS10065 and BWI76_RS10070 are separated by 89 nucleotides
BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270
_RS10050
BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074
_RS10055
BWI76_RS10060: BWI76_RS10060 - acylphosphatase, at 2,131,126 to 2,131,407
_RS10060
BWI76_RS10065: BWI76_RS10065 - sulfurtransferase TusE, at 2,131,404 to 2,131,733
_RS10065
BWI76_RS10070: BWI76_RS10070 - BAX inhibitor protein, at 2,131,823 to 2,132,482
_RS10070
Position (kb)
2131
2132 Strain fitness (log2 ratio)
-2
-1
0
1 at 2130.140 kb on + strand, within BWI76_RS10050 at 2130.164 kb on + strand, within BWI76_RS10050 at 2130.246 kb on + strand at 2130.784 kb on + strand, within BWI76_RS10055 at 2130.866 kb on + strand, within BWI76_RS10055 at 2131.088 kb on + strand at 2131.358 kb on + strand, within BWI76_RS10060 at 2132.155 kb on + strand, within BWI76_RS10070 at 2132.211 kb on - strand, within BWI76_RS10070 at 2132.355 kb on + strand, within BWI76_RS10070 at 2132.389 kb on + strand, within BWI76_RS10070
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 2,130,140 + BWI76_RS10050 0.76 -0.5 2,130,164 + BWI76_RS10050 0.81 +0.6 2,130,246 + -0.1 2,130,784 + BWI76_RS10055 0.63 +0.0 2,130,866 + BWI76_RS10055 0.74 -0.0 2,131,088 + -0.1 2,131,358 + BWI76_RS10060 0.82 -2.3 2,132,155 + BWI76_RS10070 0.50 -1.2 2,132,211 - BWI76_RS10070 0.59 +1.0 2,132,355 + BWI76_RS10070 0.81 -0.9 2,132,389 + BWI76_RS10070 0.86 -0.2
Or see this region's nucleotide sequence