Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09925 and BWI76_RS09930 are separated by 12 nucleotides BWI76_RS09930 and BWI76_RS09935 are separated by 14 nucleotides
BWI76_RS09925: BWI76_RS09925 - 23S rRNA (guanine(2445)-N(2))/(guanine(2069)-N(7))- methyltransferase, at 2,087,155 to 2,089,260
_RS09925
BWI76_RS09930: BWI76_RS09930 - ABC transporter ATPase, at 2,089,273 to 2,091,180
_RS09930
BWI76_RS09935: BWI76_RS09935 - paraquat-inducible membrane protein A, at 2,091,195 to 2,092,463
_RS09935
Position (kb)
2089
2090
2091
2092 Strain fitness (log2 ratio)
-2
-1
0
1 at 2088.679 kb on - strand, within BWI76_RS09925 at 2088.884 kb on + strand, within BWI76_RS09925 at 2089.020 kb on + strand, within BWI76_RS09925 at 2089.173 kb on - strand at 2089.481 kb on + strand, within BWI76_RS09930 at 2089.571 kb on - strand at 2089.601 kb on - strand, within BWI76_RS09930 at 2089.666 kb on - strand, within BWI76_RS09930 at 2089.707 kb on + strand, within BWI76_RS09930 at 2089.785 kb on + strand, within BWI76_RS09930 at 2089.785 kb on + strand, within BWI76_RS09930 at 2089.882 kb on - strand, within BWI76_RS09930 at 2089.971 kb on - strand, within BWI76_RS09930 at 2090.403 kb on + strand, within BWI76_RS09930 at 2090.615 kb on - strand, within BWI76_RS09930 at 2090.790 kb on + strand, within BWI76_RS09930 at 2090.848 kb on - strand, within BWI76_RS09930 at 2091.184 kb on + strand at 2091.477 kb on + strand, within BWI76_RS09935 at 2091.625 kb on - strand, within BWI76_RS09935 at 2091.988 kb on + strand, within BWI76_RS09935
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 2,088,679 - BWI76_RS09925 0.72 -1.1 2,088,884 + BWI76_RS09925 0.82 +1.0 2,089,020 + BWI76_RS09925 0.89 +0.8 2,089,173 - +0.2 2,089,481 + BWI76_RS09930 0.11 -0.9 2,089,571 - +0.2 2,089,601 - BWI76_RS09930 0.17 -1.0 2,089,666 - BWI76_RS09930 0.21 -1.1 2,089,707 + BWI76_RS09930 0.23 -0.5 2,089,785 + BWI76_RS09930 0.27 -1.6 2,089,785 + BWI76_RS09930 0.27 -0.8 2,089,882 - BWI76_RS09930 0.32 -1.1 2,089,971 - BWI76_RS09930 0.37 -2.2 2,090,403 + BWI76_RS09930 0.59 -0.2 2,090,615 - BWI76_RS09930 0.70 -0.2 2,090,790 + BWI76_RS09930 0.80 -1.2 2,090,848 - BWI76_RS09930 0.83 -1.3 2,091,184 + -1.0 2,091,477 + BWI76_RS09935 0.22 +0.8 2,091,625 - BWI76_RS09935 0.34 +0.3 2,091,988 + BWI76_RS09935 0.62 -0.4
Or see this region's nucleotide sequence