Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08015

Experiment: Chloramphenicol 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08005 and BWI76_RS08010 are separated by 137 nucleotidesBWI76_RS08010 and BWI76_RS08015 are separated by 91 nucleotidesBWI76_RS08015 and BWI76_RS08020 are separated by 106 nucleotides BWI76_RS08005: BWI76_RS08005 - transcriptional regulator, at 1,697,375 to 1,698,328 _RS08005 BWI76_RS08010: BWI76_RS08010 - octanoyltransferase, at 1,698,466 to 1,699,110 _RS08010 BWI76_RS08015: BWI76_RS08015 - hypothetical protein, at 1,699,202 to 1,699,465 _RS08015 BWI76_RS08020: BWI76_RS08020 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,699,572 to 1,700,771 _RS08020 Position (kb) 1699 1700Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1698.211 kb on - strand, within BWI76_RS08005at 1698.357 kb on + strandat 1698.357 kb on - strandat 1698.365 kb on - strandat 1698.459 kb on - strandat 1699.151 kb on + strandat 1699.755 kb on - strand, within BWI76_RS08020at 1700.122 kb on - strand, within BWI76_RS08020at 1700.205 kb on + strand, within BWI76_RS08020at 1700.215 kb on + strand, within BWI76_RS08020at 1700.223 kb on - strand, within BWI76_RS08020at 1700.259 kb on - strand, within BWI76_RS08020

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml
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1,698,211 - BWI76_RS08005 0.88 -2.3
1,698,357 + +1.4
1,698,357 - -0.0
1,698,365 - +0.1
1,698,459 - +1.1
1,699,151 + +0.6
1,699,755 - BWI76_RS08020 0.15 +1.5
1,700,122 - BWI76_RS08020 0.46 +4.4
1,700,205 + BWI76_RS08020 0.53 +0.3
1,700,215 + BWI76_RS08020 0.54 +0.4
1,700,223 - BWI76_RS08020 0.54 +0.7
1,700,259 - BWI76_RS08020 0.57 +0.6

Or see this region's nucleotide sequence