Experiment: sodium iodate 0.005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_646 and Pf6N2E2_647 are separated by 147 nucleotides Pf6N2E2_647 and Pf6N2E2_648 are separated by 47 nucleotides Pf6N2E2_648 and Pf6N2E2_649 overlap by 4 nucleotides
Pf6N2E2_646 - General secretion pathway protein I, at 735,700 to 736,074
_646
Pf6N2E2_647 - General secretion pathway protein G, at 736,222 to 736,671
_647
Pf6N2E2_648 - General secretion pathway protein K, at 736,719 to 737,666
_648
Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835
_649
Position (kb) 736
737
738 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 736.159 kb on + strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.519 kb on + strand, within Pf6N2E2_647 at 736.559 kb on - strand, within Pf6N2E2_647 at 736.564 kb on + strand, within Pf6N2E2_647 at 736.565 kb on - strand, within Pf6N2E2_647 at 736.670 kb on - strand at 736.670 kb on - strand at 737.400 kb on + strand, within Pf6N2E2_648 at 737.468 kb on + strand, within Pf6N2E2_648 at 737.618 kb on + strand at 737.662 kb on + strand at 738.077 kb on + strand, within Pf6N2E2_649 at 738.113 kb on - strand, within Pf6N2E2_649 at 738.413 kb on + strand, within Pf6N2E2_649
Per-strain Table
Position Strand Gene LocusTag Fraction sodium iodate 0.005 M remove 736,159 + +1.2 736,171 - +0.4 736,171 - -1.1 736,171 - +0.8 736,519 + Pf6N2E2_647 0.66 +0.5 736,559 - Pf6N2E2_647 0.75 -1.0 736,564 + Pf6N2E2_647 0.76 +0.3 736,565 - Pf6N2E2_647 0.76 +0.0 736,670 - +0.2 736,670 - +1.6 737,400 + Pf6N2E2_648 0.72 -3.5 737,468 + Pf6N2E2_648 0.79 -0.1 737,618 + +0.2 737,662 + -0.3 738,077 + Pf6N2E2_649 0.35 +0.6 738,113 - Pf6N2E2_649 0.38 -1.0 738,413 + Pf6N2E2_649 0.64 +0.4
Or see this region's nucleotide sequence