Experiment: Minimal media pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02295 and AO356_02300 are separated by 10 nucleotides AO356_02300 and AO356_02305 are separated by 71 nucleotides AO356_02305 and AO356_02310 are separated by 106 nucleotides AO356_02310 and AO356_02315 are separated by 236 nucleotides
AO356_02295: AO356_02295 - LexA family transcriptional regulator, at 496,975 to 497,583
_02295
AO356_02300: AO356_02300 - CDP-glycerol--UDP-pyrophosphoryl-N- acetylglucosaminyl-N-acetylmannosamine glycerophosphotransferase, at 497,594 to 498,067
_02300
AO356_02305: AO356_02305 - hypothetical protein, at 498,139 to 498,372
_02305
AO356_02310: AO356_02310 - PasA protein, at 498,479 to 499,003
_02310
AO356_02315: AO356_02315 - DNA topoisomerase I, at 499,240 to 501,852
_02315
Position (kb) 498
499
500 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 497.581 kb on + strand at 497.593 kb on + strand at 497.593 kb on + strand at 497.593 kb on + strand at 497.593 kb on + strand at 497.593 kb on + strand at 497.593 kb on + strand at 497.593 kb on + strand at 497.593 kb on + strand at 497.594 kb on - strand at 497.594 kb on - strand at 498.066 kb on - strand at 498.092 kb on + strand at 498.092 kb on + strand at 498.114 kb on + strand at 498.259 kb on + strand, within AO356_02305 at 498.259 kb on + strand, within AO356_02305 at 498.259 kb on + strand, within AO356_02305 at 498.260 kb on - strand, within AO356_02305 at 498.366 kb on + strand at 498.366 kb on + strand at 498.366 kb on + strand at 498.366 kb on + strand at 498.372 kb on + strand at 498.372 kb on + strand at 498.372 kb on + strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.373 kb on - strand at 498.403 kb on + strand at 498.403 kb on + strand at 498.403 kb on + strand at 498.403 kb on + strand at 498.404 kb on - strand at 498.404 kb on - strand at 498.404 kb on - strand at 498.404 kb on - strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.405 kb on + strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.406 kb on - strand at 498.407 kb on + strand at 498.407 kb on + strand at 498.407 kb on + strand at 498.407 kb on + strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.408 kb on - strand at 498.460 kb on + strand at 498.461 kb on - strand at 498.680 kb on + strand, within AO356_02310 at 498.680 kb on + strand, within AO356_02310 at 498.681 kb on - strand, within AO356_02310 at 498.855 kb on + strand, within AO356_02310 at 498.856 kb on - strand, within AO356_02310 at 498.963 kb on + strand at 498.987 kb on + strand at 498.987 kb on + strand at 498.987 kb on + strand at 498.988 kb on - strand at 499.107 kb on + strand at 499.107 kb on + strand at 499.107 kb on + strand at 499.108 kb on - strand at 499.149 kb on + strand at 499.150 kb on - strand at 499.158 kb on + strand at 499.158 kb on + strand at 499.159 kb on - strand at 499.159 kb on - strand at 499.159 kb on - strand at 499.159 kb on - strand at 499.281 kb on - strand at 499.281 kb on - strand at 499.281 kb on - strand at 499.325 kb on + strand at 499.410 kb on - strand at 499.532 kb on + strand, within AO356_02315 at 499.532 kb on + strand, within AO356_02315 at 499.532 kb on + strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315 at 499.533 kb on - strand, within AO356_02315
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH6 remove 497,581 + +0.8 497,593 + +0.6 497,593 + +0.6 497,593 + +1.3 497,593 + +0.4 497,593 + -0.1 497,593 + +0.3 497,593 + -0.2 497,593 + +0.1 497,594 - -0.7 497,594 - -0.2 498,066 - +0.0 498,092 + -0.8 498,092 + -0.0 498,114 + -0.2 498,259 + AO356_02305 0.51 +0.0 498,259 + AO356_02305 0.51 +0.2 498,259 + AO356_02305 0.51 -1.2 498,260 - AO356_02305 0.52 -0.1 498,366 + +0.1 498,366 + -1.5 498,366 + -2.4 498,366 + +0.9 498,372 + -0.1 498,372 + -1.7 498,372 + -0.8 498,373 - +0.1 498,373 - -1.9 498,373 - -2.0 498,373 - -1.4 498,373 - -0.8 498,373 - -0.7 498,373 - -1.1 498,403 + -1.0 498,403 + -0.6 498,403 + -1.3 498,403 + +0.3 498,404 - +0.1 498,404 - -0.9 498,404 - -0.4 498,404 - +0.9 498,405 + +0.1 498,405 + +1.4 498,405 + +0.5 498,405 + +0.2 498,405 + -0.7 498,405 + -0.9 498,405 + -1.7 498,405 + -1.4 498,405 + -0.3 498,406 - -0.5 498,406 - +0.2 498,406 - +0.4 498,406 - +0.6 498,406 - +0.3 498,406 - -0.7 498,406 - +0.7 498,406 - +0.2 498,406 - +0.3 498,406 - -1.2 498,406 - -0.6 498,406 - +0.3 498,406 - -0.0 498,407 + -0.3 498,407 + +0.5 498,407 + -0.7 498,407 + -0.0 498,408 - -0.1 498,408 - +1.1 498,408 - -0.0 498,408 - -0.7 498,408 - +0.6 498,460 + -0.8 498,461 - -1.1 498,680 + AO356_02310 0.38 -1.1 498,680 + AO356_02310 0.38 -1.0 498,681 - AO356_02310 0.38 -0.1 498,855 + AO356_02310 0.72 -2.0 498,856 - AO356_02310 0.72 -1.0 498,963 + -1.8 498,987 + -1.1 498,987 + -0.5 498,987 + -1.5 498,988 - -0.4 499,107 + +1.4 499,107 + +0.7 499,107 + -1.6 499,108 - +1.5 499,149 + -0.0 499,150 - +0.5 499,158 + +0.9 499,158 + +0.1 499,159 - +0.3 499,159 - -0.3 499,159 - +0.2 499,159 - +0.8 499,281 - -0.2 499,281 - -1.7 499,281 - -2.0 499,325 + +0.9 499,410 - -0.6 499,532 + AO356_02315 0.11 -1.6 499,532 + AO356_02315 0.11 -0.4 499,532 + AO356_02315 0.11 -0.6 499,533 - AO356_02315 0.11 -3.4 499,533 - AO356_02315 0.11 +2.0 499,533 - AO356_02315 0.11 -0.1 499,533 - AO356_02315 0.11 +0.3 499,533 - AO356_02315 0.11 -0.7
Or see this region's nucleotide sequence