Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12430

Experiment: Urea (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12420 and BWI76_RS12425 are separated by 363 nucleotidesBWI76_RS12425 and BWI76_RS12430 are separated by 169 nucleotidesBWI76_RS12430 and BWI76_RS12435 are separated by 169 nucleotides BWI76_RS12420: BWI76_RS12420 - peptide ABC transporter substrate-binding protein SapA, at 2,594,005 to 2,595,648 _RS12420 BWI76_RS12425: BWI76_RS12425 - hypothetical protein, at 2,596,012 to 2,596,296 _RS12425 BWI76_RS12430: BWI76_RS12430 - AraC family transcriptional regulator, at 2,596,466 to 2,598,772 _RS12430 BWI76_RS12435: BWI76_RS12435 - putative amino acid amidohydrolase, at 2,598,942 to 2,600,111 _RS12435 Position (kb) 2596 2597 2598 2599Strain fitness (log2 ratio) -2 -1 0 1 2at 2595.881 kb on + strandat 2595.889 kb on - strandat 2595.889 kb on - strandat 2595.889 kb on - strandat 2596.004 kb on - strandat 2596.086 kb on - strand, within BWI76_RS12425at 2596.105 kb on - strand, within BWI76_RS12425at 2596.115 kb on - strand, within BWI76_RS12425at 2596.244 kb on + strand, within BWI76_RS12425at 2596.252 kb on - strand, within BWI76_RS12425at 2596.594 kb on + strandat 2596.728 kb on + strand, within BWI76_RS12430at 2597.157 kb on - strand, within BWI76_RS12430at 2597.377 kb on - strand, within BWI76_RS12430at 2597.428 kb on + strand, within BWI76_RS12430at 2597.566 kb on - strand, within BWI76_RS12430at 2597.648 kb on - strand, within BWI76_RS12430at 2597.733 kb on + strand, within BWI76_RS12430at 2597.844 kb on + strand, within BWI76_RS12430at 2597.875 kb on - strand, within BWI76_RS12430at 2597.889 kb on + strand, within BWI76_RS12430at 2598.015 kb on - strand, within BWI76_RS12430at 2598.102 kb on + strand, within BWI76_RS12430at 2598.151 kb on + strand, within BWI76_RS12430at 2598.151 kb on + strand, within BWI76_RS12430at 2598.344 kb on - strand, within BWI76_RS12430at 2598.459 kb on - strand, within BWI76_RS12430at 2598.543 kb on - strandat 2598.719 kb on + strandat 2598.920 kb on + strandat 2598.969 kb on - strandat 2599.137 kb on - strand, within BWI76_RS12435at 2599.211 kb on + strand, within BWI76_RS12435at 2599.219 kb on - strand, within BWI76_RS12435at 2599.285 kb on - strand, within BWI76_RS12435at 2599.366 kb on - strand, within BWI76_RS12435at 2599.366 kb on - strand, within BWI76_RS12435at 2599.366 kb on - strand, within BWI76_RS12435at 2599.381 kb on - strand, within BWI76_RS12435at 2599.657 kb on + strand, within BWI76_RS12435at 2599.664 kb on + strand, within BWI76_RS12435

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Per-strain Table

Position Strand Gene LocusTag Fraction Urea (N)
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2,595,881 + +1.0
2,595,889 - -0.3
2,595,889 - -0.2
2,595,889 - -2.1
2,596,004 - -0.5
2,596,086 - BWI76_RS12425 0.26 +0.0
2,596,105 - BWI76_RS12425 0.33 +0.2
2,596,115 - BWI76_RS12425 0.36 -0.1
2,596,244 + BWI76_RS12425 0.81 -0.1
2,596,252 - BWI76_RS12425 0.84 +0.0
2,596,594 + +0.5
2,596,728 + BWI76_RS12430 0.11 +0.3
2,597,157 - BWI76_RS12430 0.30 +0.1
2,597,377 - BWI76_RS12430 0.39 +0.3
2,597,428 + BWI76_RS12430 0.42 +0.0
2,597,566 - BWI76_RS12430 0.48 +1.3
2,597,648 - BWI76_RS12430 0.51 +1.0
2,597,733 + BWI76_RS12430 0.55 +0.7
2,597,844 + BWI76_RS12430 0.60 +0.2
2,597,875 - BWI76_RS12430 0.61 +0.1
2,597,889 + BWI76_RS12430 0.62 +0.3
2,598,015 - BWI76_RS12430 0.67 +1.7
2,598,102 + BWI76_RS12430 0.71 +0.6
2,598,151 + BWI76_RS12430 0.73 +0.7
2,598,151 + BWI76_RS12430 0.73 +0.4
2,598,344 - BWI76_RS12430 0.81 -0.8
2,598,459 - BWI76_RS12430 0.86 +0.2
2,598,543 - -0.8
2,598,719 + +2.2
2,598,920 + +0.3
2,598,969 - +1.1
2,599,137 - BWI76_RS12435 0.17 +0.2
2,599,211 + BWI76_RS12435 0.23 +0.4
2,599,219 - BWI76_RS12435 0.24 -0.6
2,599,285 - BWI76_RS12435 0.29 -2.4
2,599,366 - BWI76_RS12435 0.36 +0.6
2,599,366 - BWI76_RS12435 0.36 +0.1
2,599,366 - BWI76_RS12435 0.36 +1.0
2,599,381 - BWI76_RS12435 0.38 -0.3
2,599,657 + BWI76_RS12435 0.61 -0.3
2,599,664 + BWI76_RS12435 0.62 -0.4

Or see this region's nucleotide sequence