Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01285

Experiment: Urea (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01280 and BWI76_RS01285 are separated by 165 nucleotidesBWI76_RS01285 and BWI76_RS01290 are separated by 3 nucleotides BWI76_RS01280: BWI76_RS01280 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 261,981 to 264,242 _RS01280 BWI76_RS01285: BWI76_RS01285 - amino acid ABC transporter substrate-binding protein, at 264,408 to 265,097 _RS01285 BWI76_RS01290: BWI76_RS01290 - putative dienelactone hydrolase, at 265,101 to 265,913 _RS01290 Position (kb) 264 265 266Strain fitness (log2 ratio) -1 0 1at 263.459 kb on - strand, within BWI76_RS01280at 263.513 kb on - strand, within BWI76_RS01280at 263.518 kb on - strand, within BWI76_RS01280at 263.525 kb on - strand, within BWI76_RS01280at 263.535 kb on - strand, within BWI76_RS01280at 263.901 kb on + strand, within BWI76_RS01280at 263.906 kb on + strand, within BWI76_RS01280at 264.050 kb on + strandat 264.087 kb on - strandat 264.104 kb on + strandat 264.142 kb on + strandat 264.217 kb on - strandat 264.217 kb on - strandat 264.217 kb on - strandat 264.319 kb on - strandat 264.367 kb on - strandat 264.448 kb on + strandat 264.505 kb on + strand, within BWI76_RS01285at 264.568 kb on - strand, within BWI76_RS01285at 264.662 kb on - strand, within BWI76_RS01285at 264.761 kb on - strand, within BWI76_RS01285at 264.958 kb on + strand, within BWI76_RS01285at 264.992 kb on + strand, within BWI76_RS01285at 265.007 kb on - strand, within BWI76_RS01285at 265.060 kb on - strandat 265.099 kb on - strandat 265.132 kb on - strandat 265.279 kb on + strand, within BWI76_RS01290at 265.420 kb on + strand, within BWI76_RS01290at 265.420 kb on + strand, within BWI76_RS01290at 265.444 kb on + strand, within BWI76_RS01290at 265.468 kb on + strand, within BWI76_RS01290at 265.545 kb on - strand, within BWI76_RS01290at 265.846 kb on - strandat 265.961 kb on + strandat 265.961 kb on + strandat 266.033 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Urea (N)
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263,459 - BWI76_RS01280 0.65 +0.9
263,513 - BWI76_RS01280 0.68 +0.3
263,518 - BWI76_RS01280 0.68 +0.7
263,525 - BWI76_RS01280 0.68 -0.1
263,535 - BWI76_RS01280 0.69 -0.9
263,901 + BWI76_RS01280 0.85 -0.3
263,906 + BWI76_RS01280 0.85 -0.3
264,050 + +1.5
264,087 - -0.2
264,104 + -0.3
264,142 + +0.5
264,217 - +0.3
264,217 - -0.5
264,217 - -1.6
264,319 - -0.4
264,367 - -1.1
264,448 + +0.2
264,505 + BWI76_RS01285 0.14 -0.9
264,568 - BWI76_RS01285 0.23 +1.1
264,662 - BWI76_RS01285 0.37 -0.2
264,761 - BWI76_RS01285 0.51 +0.9
264,958 + BWI76_RS01285 0.80 +0.6
264,992 + BWI76_RS01285 0.85 +0.4
265,007 - BWI76_RS01285 0.87 +0.4
265,060 - +1.2
265,099 - +0.1
265,132 - +0.2
265,279 + BWI76_RS01290 0.22 -0.0
265,420 + BWI76_RS01290 0.39 +0.1
265,420 + BWI76_RS01290 0.39 +0.2
265,444 + BWI76_RS01290 0.42 +0.1
265,468 + BWI76_RS01290 0.45 +0.4
265,545 - BWI76_RS01290 0.55 +0.2
265,846 - +0.3
265,961 + +1.2
265,961 + -0.4
266,033 - +1.0

Or see this region's nucleotide sequence