Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_495 and Pf6N2E2_496 are separated by 183 nucleotides Pf6N2E2_496 and Pf6N2E2_497 are separated by 0 nucleotides
Pf6N2E2_495 - YD repeat protein, at 561,448 to 562,563
_495
Pf6N2E2_496 - Dihydropyrimidine dehydrogenase (NADP(+)) (EC 1.3.1.2) <small>(from data)</small>, at 562,747 to 564,021
_496
Pf6N2E2_497 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 564,022 to 565,389
_497
Position (kb) 562
563
564
565 Strain fitness (log2 ratio) -2
-1
0
1
2 at 561.834 kb on + strand, within Pf6N2E2_495 at 562.001 kb on + strand, within Pf6N2E2_495 at 562.085 kb on + strand, within Pf6N2E2_495 at 562.184 kb on + strand, within Pf6N2E2_495 at 562.184 kb on + strand, within Pf6N2E2_495 at 562.607 kb on + strand at 562.625 kb on + strand at 562.628 kb on + strand at 562.640 kb on + strand at 562.640 kb on + strand at 562.640 kb on + strand at 562.640 kb on + strand at 562.641 kb on - strand at 562.764 kb on - strand at 562.867 kb on - strand at 562.892 kb on - strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.898 kb on + strand, within Pf6N2E2_496 at 562.899 kb on - strand, within Pf6N2E2_496 at 562.902 kb on + strand, within Pf6N2E2_496 at 562.902 kb on + strand, within Pf6N2E2_496 at 562.902 kb on + strand, within Pf6N2E2_496 at 562.903 kb on - strand at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 562.903 kb on - strand, within Pf6N2E2_496 at 563.021 kb on + strand, within Pf6N2E2_496 at 563.021 kb on + strand, within Pf6N2E2_496 at 563.022 kb on - strand, within Pf6N2E2_496 at 563.022 kb on - strand, within Pf6N2E2_496 at 563.022 kb on - strand, within Pf6N2E2_496 at 563.036 kb on + strand, within Pf6N2E2_496 at 563.036 kb on + strand, within Pf6N2E2_496 at 563.036 kb on + strand, within Pf6N2E2_496 at 563.522 kb on + strand, within Pf6N2E2_496 at 563.522 kb on + strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.523 kb on - strand, within Pf6N2E2_496 at 563.555 kb on + strand, within Pf6N2E2_496 at 563.555 kb on + strand, within Pf6N2E2_496 at 563.556 kb on - strand, within Pf6N2E2_496 at 563.556 kb on - strand, within Pf6N2E2_496 at 563.843 kb on + strand, within Pf6N2E2_496 at 563.936 kb on + strand at 563.937 kb on - strand at 563.937 kb on - strand at 563.937 kb on - strand at 563.937 kb on - strand at 563.937 kb on - strand at 564.024 kb on - strand at 564.141 kb on - strand at 564.141 kb on - strand at 564.617 kb on + strand, within Pf6N2E2_497 at 564.617 kb on + strand, within Pf6N2E2_497 at 564.815 kb on + strand, within Pf6N2E2_497 at 564.815 kb on + strand, within Pf6N2E2_497 at 564.833 kb on + strand, within Pf6N2E2_497 at 564.834 kb on - strand, within Pf6N2E2_497 at 564.834 kb on - strand, within Pf6N2E2_497 at 564.834 kb on - strand, within Pf6N2E2_497
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 561,834 + Pf6N2E2_495 0.35 +1.1 562,001 + Pf6N2E2_495 0.50 -0.0 562,085 + Pf6N2E2_495 0.57 +0.1 562,184 + Pf6N2E2_495 0.66 -0.9 562,184 + Pf6N2E2_495 0.66 +0.4 562,607 + +1.9 562,625 + +0.8 562,628 + +1.3 562,640 + -0.1 562,640 + -0.3 562,640 + -1.3 562,640 + -0.1 562,641 - -0.0 562,764 - -0.1 562,867 - +0.5 562,892 - Pf6N2E2_496 0.11 -0.1 562,898 + Pf6N2E2_496 0.12 +0.5 562,898 + Pf6N2E2_496 0.12 +1.9 562,898 + Pf6N2E2_496 0.12 -0.7 562,898 + Pf6N2E2_496 0.12 -0.5 562,898 + Pf6N2E2_496 0.12 +0.4 562,898 + Pf6N2E2_496 0.12 -1.5 562,899 - Pf6N2E2_496 0.12 +1.6 562,902 + Pf6N2E2_496 0.12 +0.2 562,902 + Pf6N2E2_496 0.12 -1.0 562,902 + Pf6N2E2_496 0.12 -2.2 562,903 - -0.7 562,903 - Pf6N2E2_496 0.12 -0.1 562,903 - Pf6N2E2_496 0.12 -0.5 562,903 - Pf6N2E2_496 0.12 -0.7 562,903 - Pf6N2E2_496 0.12 +0.2 562,903 - Pf6N2E2_496 0.12 +2.1 563,021 + Pf6N2E2_496 0.21 -1.0 563,021 + Pf6N2E2_496 0.21 -0.4 563,022 - Pf6N2E2_496 0.22 +1.5 563,022 - Pf6N2E2_496 0.22 +0.9 563,022 - Pf6N2E2_496 0.22 +0.3 563,036 + Pf6N2E2_496 0.23 -0.5 563,036 + Pf6N2E2_496 0.23 -2.1 563,036 + Pf6N2E2_496 0.23 -0.5 563,522 + Pf6N2E2_496 0.61 +0.6 563,522 + Pf6N2E2_496 0.61 -0.1 563,523 - Pf6N2E2_496 0.61 -1.3 563,523 - Pf6N2E2_496 0.61 -0.7 563,523 - Pf6N2E2_496 0.61 -0.7 563,523 - Pf6N2E2_496 0.61 -1.6 563,523 - Pf6N2E2_496 0.61 -0.0 563,523 - Pf6N2E2_496 0.61 -0.7 563,555 + Pf6N2E2_496 0.63 -0.7 563,555 + Pf6N2E2_496 0.63 -1.0 563,556 - Pf6N2E2_496 0.63 +0.9 563,556 - Pf6N2E2_496 0.63 +0.3 563,843 + Pf6N2E2_496 0.86 +0.1 563,936 + -0.5 563,937 - +1.4 563,937 - +0.9 563,937 - +0.1 563,937 - +1.1 563,937 - -0.7 564,024 - +0.6 564,141 - -0.3 564,141 - -1.5 564,617 + Pf6N2E2_497 0.43 +1.5 564,617 + Pf6N2E2_497 0.43 +1.9 564,815 + Pf6N2E2_497 0.58 -0.1 564,815 + Pf6N2E2_497 0.58 +0.3 564,833 + Pf6N2E2_497 0.59 +1.4 564,834 - Pf6N2E2_497 0.59 +0.2 564,834 - Pf6N2E2_497 0.59 -0.1 564,834 - Pf6N2E2_497 0.59 -0.6
Or see this region's nucleotide sequence