Experiment: thiosulfate 0.25 M 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf6N2E2_4437 and Pf6N2E2_4438 overlap by 4 nucleotides Pf6N2E2_4438 and Pf6N2E2_4439 are separated by 50 nucleotides Pf6N2E2_4439 and Pf6N2E2_4440 overlap by 1 nucleotides Pf6N2E2_4440 and Pf6N2E2_4441 are separated by 143 nucleotides  
        
        Pf6N2E2_4437 - Twin-arginine translocation protein TatB, at 5,168,942 to 5,170,117 
         
        _4437 
         
        
        Pf6N2E2_4438 - hypothetical protein, at 5,170,114 to 5,170,578 
         
        _4438 
         
        
        Pf6N2E2_4439 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 5,170,629 to 5,172,539 
         
        _4439 
         
        
        Pf6N2E2_4440 - Small-conductance mechanosensitive channel, at 5,172,539 to 5,173,120 
         
        _4440 
         
        
        Pf6N2E2_4441 - Homoserine/homoserine lactone efflux protein, at 5,173,264 to 5,173,896 
         
        _4441 
         Position (kb)  
5170 
 
5171 
 
5172 
 
5173 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 at 5169.647 kb on - strand, within Pf6N2E2_4437 at 5169.685 kb on - strand, within Pf6N2E2_4437 at 5170.115 kb on + strand at 5170.115 kb on + strand at 5170.116 kb on - strand at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.406 kb on - strand, within Pf6N2E2_4438 at 5170.406 kb on - strand, within Pf6N2E2_4438 at 5170.525 kb on + strand, within Pf6N2E2_4438 at 5170.526 kb on - strand, within Pf6N2E2_4438 at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.584 kb on + strand at 5170.612 kb on + strand at 5170.612 kb on + strand at 5170.628 kb on + strand at 5170.629 kb on - strand at 5170.629 kb on - strand at 5170.654 kb on + strand at 5170.654 kb on + strand at 5170.655 kb on - strand at 5170.678 kb on + strand at 5170.678 kb on + strand at 5170.679 kb on - strand at 5170.679 kb on - strand at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.876 kb on - strand, within Pf6N2E2_4439 at 5170.876 kb on - strand, within Pf6N2E2_4439 at 5170.995 kb on + strand, within Pf6N2E2_4439 at 5170.996 kb on - strand, within Pf6N2E2_4439 at 5171.042 kb on + strand, within Pf6N2E2_4439 at 5171.307 kb on + strand, within Pf6N2E2_4439 at 5171.307 kb on + strand, within Pf6N2E2_4439 at 5171.308 kb on - strand, within Pf6N2E2_4439 at 5171.321 kb on + strand, within Pf6N2E2_4439 at 5171.373 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.413 kb on - strand, within Pf6N2E2_4439 at 5171.413 kb on - strand, within Pf6N2E2_4439 at 5171.678 kb on + strand, within Pf6N2E2_4439 at 5171.678 kb on + strand, within Pf6N2E2_4439 at 5172.171 kb on + strand, within Pf6N2E2_4439 at 5172.171 kb on + strand, within Pf6N2E2_4439 at 5172.172 kb on - strand, within Pf6N2E2_4439 at 5172.394 kb on - strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.560 kb on - strand at 5172.789 kb on + strand, within Pf6N2E2_4440 at 5172.790 kb on - strand, within Pf6N2E2_4440 at 5172.853 kb on - strand, within Pf6N2E2_4440 at 5172.884 kb on + strand, within Pf6N2E2_4440 at 5172.884 kb on + strand, within Pf6N2E2_4440 at 5172.884 kb on + strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.983 kb on + strand, within Pf6N2E2_4440 at 5172.983 kb on + strand, within Pf6N2E2_4440 at 5172.983 kb on + strand, within Pf6N2E2_4440 at 5172.984 kb on - strand, within Pf6N2E2_4440 at 5172.984 kb on - strand, within Pf6N2E2_4440 at 5172.984 kb on - strand, within Pf6N2E2_4440 at 5173.038 kb on + strand, within Pf6N2E2_4440 at 5173.038 kb on + strand, within Pf6N2E2_4440 at 5173.118 kb on + strand at 5173.118 kb on + strand at 5173.130 kb on - strand at 5173.180 kb on - strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.229 kb on + strand at 5173.230 kb on - strand at 5173.480 kb on + strand, within Pf6N2E2_4441 at 5173.480 kb on + strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.507 kb on + strand, within Pf6N2E2_4441 at 5173.507 kb on + strand, within Pf6N2E2_4441 at 5173.516 kb on + strand, within Pf6N2E2_4441  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   thiosulfate 0.25 M             remove   5,169,647   -    Pf6N2E2_4437   0.60  -0.5   5,169,685   -    Pf6N2E2_4437   0.63  -1.3   5,170,115   +        +0.6   5,170,115   +        -1.9   5,170,116   -        +0.5   5,170,405   +    Pf6N2E2_4438   0.63  -1.1   5,170,405   +    Pf6N2E2_4438   0.63  -2.8   5,170,405   +    Pf6N2E2_4438   0.63  -0.1   5,170,406   -    Pf6N2E2_4438   0.63  -1.2   5,170,406   -    Pf6N2E2_4438   0.63  +0.2   5,170,525   +    Pf6N2E2_4438   0.88  -0.3   5,170,526   -    Pf6N2E2_4438   0.89  +0.7   5,170,541   +        +1.5   5,170,541   +        +0.2   5,170,541   +        +1.1   5,170,541   +        -0.4   5,170,584   +        +1.6   5,170,612   +        +0.9   5,170,612   +        -0.1   5,170,628   +        +0.3   5,170,629   -        +0.6   5,170,629   -        +0.1   5,170,654   +        +0.5   5,170,654   +        +0.5   5,170,655   -        +0.6   5,170,678   +        -1.3   5,170,678   +        -0.7   5,170,679   -        +0.3   5,170,679   -        -1.3   5,170,875   +    Pf6N2E2_4439   0.13  -1.4   5,170,875   +    Pf6N2E2_4439   0.13  +0.3   5,170,875   +    Pf6N2E2_4439   0.13  +0.2   5,170,875   +    Pf6N2E2_4439   0.13  -0.5   5,170,876   -    Pf6N2E2_4439   0.13  -1.0   5,170,876   -    Pf6N2E2_4439   0.13  -0.3   5,170,995   +    Pf6N2E2_4439   0.19  -0.0   5,170,996   -    Pf6N2E2_4439   0.19  -0.2   5,171,042   +    Pf6N2E2_4439   0.22  +1.4   5,171,307   +    Pf6N2E2_4439   0.35  -0.4   5,171,307   +    Pf6N2E2_4439   0.35  +0.1   5,171,308   -    Pf6N2E2_4439   0.36  -2.1   5,171,321   +    Pf6N2E2_4439   0.36  -0.3   5,171,373   +    Pf6N2E2_4439   0.39  +0.4   5,171,412   +    Pf6N2E2_4439   0.41  -0.6   5,171,412   +    Pf6N2E2_4439   0.41  +0.4   5,171,412   +    Pf6N2E2_4439   0.41  +0.9   5,171,412   +    Pf6N2E2_4439   0.41  +0.3   5,171,412   +    Pf6N2E2_4439   0.41  -2.1   5,171,413   -    Pf6N2E2_4439   0.41  -0.6   5,171,413   -    Pf6N2E2_4439   0.41  +0.7   5,171,678   +    Pf6N2E2_4439   0.55  -0.1   5,171,678   +    Pf6N2E2_4439   0.55  -0.2   5,172,171   +    Pf6N2E2_4439   0.81  +1.9   5,172,171   +    Pf6N2E2_4439   0.81  +1.1   5,172,172   -    Pf6N2E2_4439   0.81  -0.2   5,172,394   -        +0.4   5,172,559   +        +2.2   5,172,559   +        +0.1   5,172,559   +        +1.8   5,172,559   +        +0.6   5,172,559   +        -0.3   5,172,559   +        -0.6   5,172,560   -        -1.0   5,172,789   +    Pf6N2E2_4440   0.43  -0.0   5,172,790   -    Pf6N2E2_4440   0.43  +0.8   5,172,853   -    Pf6N2E2_4440   0.54  +0.3   5,172,884   +    Pf6N2E2_4440   0.59  +1.8   5,172,884   +    Pf6N2E2_4440   0.59  -0.9   5,172,884   +    Pf6N2E2_4440   0.59  -0.3   5,172,885   -    Pf6N2E2_4440   0.59  +0.1   5,172,885   -    Pf6N2E2_4440   0.59  -2.0   5,172,885   -    Pf6N2E2_4440   0.59  -0.2   5,172,885   -    Pf6N2E2_4440   0.59  -0.4   5,172,885   -    Pf6N2E2_4440   0.59  -1.5   5,172,983   +    Pf6N2E2_4440   0.76  +1.7   5,172,983   +    Pf6N2E2_4440   0.76  +0.8   5,172,983   +    Pf6N2E2_4440   0.76  +0.5   5,172,984   -    Pf6N2E2_4440   0.76  -0.4   5,172,984   -    Pf6N2E2_4440   0.76  -0.5   5,172,984   -    Pf6N2E2_4440   0.76  -0.0   5,173,038   +    Pf6N2E2_4440   0.86  +0.3   5,173,038   +    Pf6N2E2_4440   0.86  -0.5   5,173,118   +        +1.0   5,173,118   +        +0.9   5,173,130   -        +1.3   5,173,180   -        -2.6   5,173,224   +        +0.3   5,173,224   +        -0.3   5,173,224   +        -0.6   5,173,224   +        +0.1   5,173,224   +        +0.7   5,173,225   -        -1.5   5,173,225   -        +0.9   5,173,225   -        +1.6   5,173,225   -        -0.7   5,173,225   -        -0.5   5,173,225   -        +0.3   5,173,229   +        -0.5   5,173,230   -        -0.5   5,173,480   +    Pf6N2E2_4441   0.34  -1.0   5,173,480   +    Pf6N2E2_4441   0.34  -0.1   5,173,481   -    Pf6N2E2_4441   0.34  +0.7   5,173,481   -    Pf6N2E2_4441   0.34  -0.7   5,173,481   -    Pf6N2E2_4441   0.34  -1.0   5,173,481   -    Pf6N2E2_4441   0.34  -0.2   5,173,481   -    Pf6N2E2_4441   0.34  -1.4   5,173,507   +    Pf6N2E2_4441   0.38  +0.1   5,173,507   +    Pf6N2E2_4441   0.38  -1.7   5,173,516   +    Pf6N2E2_4441   0.40  +0.5 
 
Or see this region's nucleotide sequence