Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4437 and Pf6N2E2_4438 overlap by 4 nucleotides Pf6N2E2_4438 and Pf6N2E2_4439 are separated by 50 nucleotides
Pf6N2E2_4437 - Twin-arginine translocation protein TatB, at 5,168,942 to 5,170,117
_4437
Pf6N2E2_4438 - hypothetical protein, at 5,170,114 to 5,170,578
_4438
Pf6N2E2_4439 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 5,170,629 to 5,172,539
_4439
Position (kb) 5170
5171 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5169.242 kb on + strand, within Pf6N2E2_4437 at 5169.242 kb on + strand, within Pf6N2E2_4437 at 5169.242 kb on + strand, within Pf6N2E2_4437 at 5169.242 kb on + strand, within Pf6N2E2_4437 at 5169.242 kb on + strand, within Pf6N2E2_4437 at 5169.243 kb on - strand, within Pf6N2E2_4437 at 5169.243 kb on - strand, within Pf6N2E2_4437 at 5169.243 kb on - strand, within Pf6N2E2_4437 at 5169.243 kb on - strand, within Pf6N2E2_4437 at 5169.243 kb on - strand, within Pf6N2E2_4437 at 5169.598 kb on + strand, within Pf6N2E2_4437 at 5169.598 kb on + strand, within Pf6N2E2_4437 at 5169.611 kb on - strand, within Pf6N2E2_4437 at 5169.647 kb on - strand, within Pf6N2E2_4437 at 5169.685 kb on - strand, within Pf6N2E2_4437 at 5170.115 kb on + strand at 5170.115 kb on + strand at 5170.116 kb on - strand at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.406 kb on - strand, within Pf6N2E2_4438 at 5170.406 kb on - strand, within Pf6N2E2_4438 at 5170.525 kb on + strand, within Pf6N2E2_4438 at 5170.526 kb on - strand, within Pf6N2E2_4438 at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.584 kb on + strand at 5170.612 kb on + strand at 5170.612 kb on + strand at 5170.628 kb on + strand at 5170.629 kb on - strand at 5170.629 kb on - strand at 5170.654 kb on + strand at 5170.654 kb on + strand at 5170.655 kb on - strand at 5170.678 kb on + strand at 5170.678 kb on + strand at 5170.679 kb on - strand at 5170.679 kb on - strand at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.876 kb on - strand, within Pf6N2E2_4439 at 5170.876 kb on - strand, within Pf6N2E2_4439 at 5170.995 kb on + strand, within Pf6N2E2_4439 at 5170.996 kb on - strand, within Pf6N2E2_4439 at 5171.042 kb on + strand, within Pf6N2E2_4439 at 5171.307 kb on + strand, within Pf6N2E2_4439 at 5171.307 kb on + strand, within Pf6N2E2_4439 at 5171.308 kb on - strand, within Pf6N2E2_4439 at 5171.321 kb on + strand, within Pf6N2E2_4439 at 5171.373 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.413 kb on - strand, within Pf6N2E2_4439 at 5171.413 kb on - strand, within Pf6N2E2_4439
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 5,169,242 + Pf6N2E2_4437 0.26 -0.2 5,169,242 + Pf6N2E2_4437 0.26 -0.6 5,169,242 + Pf6N2E2_4437 0.26 -1.9 5,169,242 + Pf6N2E2_4437 0.26 +0.3 5,169,242 + Pf6N2E2_4437 0.26 -1.3 5,169,243 - Pf6N2E2_4437 0.26 -0.4 5,169,243 - Pf6N2E2_4437 0.26 -1.7 5,169,243 - Pf6N2E2_4437 0.26 -1.1 5,169,243 - Pf6N2E2_4437 0.26 -0.4 5,169,243 - Pf6N2E2_4437 0.26 -0.8 5,169,598 + Pf6N2E2_4437 0.56 +0.1 5,169,598 + Pf6N2E2_4437 0.56 -0.4 5,169,611 - Pf6N2E2_4437 0.57 -0.1 5,169,647 - Pf6N2E2_4437 0.60 -0.5 5,169,685 - Pf6N2E2_4437 0.63 -1.3 5,170,115 + +0.6 5,170,115 + -1.9 5,170,116 - +0.5 5,170,405 + Pf6N2E2_4438 0.63 -1.1 5,170,405 + Pf6N2E2_4438 0.63 -2.8 5,170,405 + Pf6N2E2_4438 0.63 -0.1 5,170,406 - Pf6N2E2_4438 0.63 -1.2 5,170,406 - Pf6N2E2_4438 0.63 +0.2 5,170,525 + Pf6N2E2_4438 0.88 -0.3 5,170,526 - Pf6N2E2_4438 0.89 +0.7 5,170,541 + +1.5 5,170,541 + +0.2 5,170,541 + +1.1 5,170,541 + -0.4 5,170,584 + +1.6 5,170,612 + +0.9 5,170,612 + -0.1 5,170,628 + +0.3 5,170,629 - +0.6 5,170,629 - +0.1 5,170,654 + +0.5 5,170,654 + +0.5 5,170,655 - +0.6 5,170,678 + -1.3 5,170,678 + -0.7 5,170,679 - +0.3 5,170,679 - -1.3 5,170,875 + Pf6N2E2_4439 0.13 -1.4 5,170,875 + Pf6N2E2_4439 0.13 +0.3 5,170,875 + Pf6N2E2_4439 0.13 +0.2 5,170,875 + Pf6N2E2_4439 0.13 -0.5 5,170,876 - Pf6N2E2_4439 0.13 -1.0 5,170,876 - Pf6N2E2_4439 0.13 -0.3 5,170,995 + Pf6N2E2_4439 0.19 -0.0 5,170,996 - Pf6N2E2_4439 0.19 -0.2 5,171,042 + Pf6N2E2_4439 0.22 +1.4 5,171,307 + Pf6N2E2_4439 0.35 -0.4 5,171,307 + Pf6N2E2_4439 0.35 +0.1 5,171,308 - Pf6N2E2_4439 0.36 -2.1 5,171,321 + Pf6N2E2_4439 0.36 -0.3 5,171,373 + Pf6N2E2_4439 0.39 +0.4 5,171,412 + Pf6N2E2_4439 0.41 -0.6 5,171,412 + Pf6N2E2_4439 0.41 +0.4 5,171,412 + Pf6N2E2_4439 0.41 +0.9 5,171,412 + Pf6N2E2_4439 0.41 +0.3 5,171,412 + Pf6N2E2_4439 0.41 -2.1 5,171,413 - Pf6N2E2_4439 0.41 -0.6 5,171,413 - Pf6N2E2_4439 0.41 +0.7
Or see this region's nucleotide sequence