Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4310 and Pf6N2E2_4311 are separated by 104 nucleotides Pf6N2E2_4311 and Pf6N2E2_4312 overlap by 4 nucleotides
Pf6N2E2_4310 - IcmF-related protein, at 5,025,752 to 5,029,195
_4310
Pf6N2E2_4311 - Protein serine/threonine phosphatase PrpC, regulation of stationary phase, at 5,029,300 to 5,029,917
_4311
Pf6N2E2_4312 - Serine/threonine protein kinase (EC 2.7.11.1), at 5,029,914 to 5,030,846
_4312
Position (kb)
5029
5030 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5028.474 kb on + strand, within Pf6N2E2_4310 at 5028.488 kb on + strand, within Pf6N2E2_4310 at 5028.489 kb on - strand, within Pf6N2E2_4310 at 5028.489 kb on - strand, within Pf6N2E2_4310 at 5028.525 kb on - strand, within Pf6N2E2_4310 at 5028.525 kb on - strand, within Pf6N2E2_4310 at 5028.534 kb on - strand, within Pf6N2E2_4310 at 5028.631 kb on - strand, within Pf6N2E2_4310 at 5028.631 kb on - strand, within Pf6N2E2_4310 at 5028.631 kb on - strand, within Pf6N2E2_4310 at 5028.653 kb on + strand, within Pf6N2E2_4310 at 5028.654 kb on - strand, within Pf6N2E2_4310 at 5028.654 kb on - strand, within Pf6N2E2_4310 at 5028.689 kb on + strand, within Pf6N2E2_4310 at 5028.737 kb on + strand, within Pf6N2E2_4310 at 5028.738 kb on - strand, within Pf6N2E2_4310 at 5028.810 kb on + strand, within Pf6N2E2_4310 at 5028.811 kb on - strand, within Pf6N2E2_4310 at 5028.811 kb on - strand, within Pf6N2E2_4310 at 5028.811 kb on - strand, within Pf6N2E2_4310 at 5028.830 kb on + strand, within Pf6N2E2_4310 at 5028.830 kb on + strand, within Pf6N2E2_4310 at 5028.831 kb on - strand, within Pf6N2E2_4310 at 5028.890 kb on + strand at 5028.922 kb on + strand at 5028.922 kb on + strand at 5028.961 kb on - strand at 5029.055 kb on + strand at 5029.115 kb on + strand at 5029.115 kb on + strand at 5029.116 kb on - strand at 5029.116 kb on - strand at 5029.116 kb on - strand at 5029.116 kb on - strand at 5029.116 kb on - strand at 5029.135 kb on + strand at 5029.213 kb on + strand at 5029.214 kb on - strand at 5029.254 kb on + strand at 5029.255 kb on - strand at 5029.255 kb on - strand at 5029.562 kb on - strand, within Pf6N2E2_4311 at 5029.562 kb on - strand, within Pf6N2E2_4311 at 5029.562 kb on - strand, within Pf6N2E2_4311 at 5029.585 kb on + strand, within Pf6N2E2_4311 at 5029.585 kb on + strand, within Pf6N2E2_4311 at 5029.585 kb on + strand, within Pf6N2E2_4311 at 5029.585 kb on + strand, within Pf6N2E2_4311 at 5029.585 kb on + strand, within Pf6N2E2_4311 at 5029.586 kb on - strand, within Pf6N2E2_4311 at 5029.586 kb on - strand, within Pf6N2E2_4311 at 5029.586 kb on - strand, within Pf6N2E2_4311 at 5029.586 kb on - strand, within Pf6N2E2_4311 at 5029.586 kb on - strand, within Pf6N2E2_4311 at 5029.780 kb on + strand, within Pf6N2E2_4311 at 5029.780 kb on + strand, within Pf6N2E2_4311 at 5029.780 kb on + strand, within Pf6N2E2_4311 at 5029.780 kb on + strand, within Pf6N2E2_4311 at 5029.780 kb on + strand, within Pf6N2E2_4311 at 5029.781 kb on - strand, within Pf6N2E2_4311 at 5029.941 kb on + strand at 5029.941 kb on + strand at 5029.941 kb on + strand at 5029.941 kb on + strand at 5029.942 kb on - strand at 5030.011 kb on + strand, within Pf6N2E2_4312 at 5030.011 kb on + strand, within Pf6N2E2_4312 at 5030.118 kb on + strand, within Pf6N2E2_4312 at 5030.119 kb on - strand, within Pf6N2E2_4312 at 5030.223 kb on - strand, within Pf6N2E2_4312 at 5030.235 kb on + strand, within Pf6N2E2_4312 at 5030.235 kb on + strand, within Pf6N2E2_4312 at 5030.236 kb on - strand, within Pf6N2E2_4312 at 5030.391 kb on + strand, within Pf6N2E2_4312 at 5030.392 kb on - strand, within Pf6N2E2_4312 at 5030.410 kb on + strand, within Pf6N2E2_4312 at 5030.410 kb on + strand, within Pf6N2E2_4312 at 5030.410 kb on + strand, within Pf6N2E2_4312 at 5030.614 kb on - strand, within Pf6N2E2_4312 at 5030.614 kb on - strand, within Pf6N2E2_4312 at 5030.634 kb on + strand, within Pf6N2E2_4312 at 5030.634 kb on + strand, within Pf6N2E2_4312 at 5030.634 kb on + strand, within Pf6N2E2_4312 at 5030.635 kb on - strand, within Pf6N2E2_4312 at 5030.635 kb on - strand, within Pf6N2E2_4312 at 5030.635 kb on - strand, within Pf6N2E2_4312 at 5030.635 kb on - strand, within Pf6N2E2_4312 at 5030.671 kb on + strand, within Pf6N2E2_4312 at 5030.793 kb on - strand at 5030.883 kb on + strand at 5030.883 kb on + strand at 5030.884 kb on - strand at 5030.884 kb on - strand at 5030.884 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 5,028,474 + Pf6N2E2_4310 0.79 +0.0 5,028,488 + Pf6N2E2_4310 0.79 +1.0 5,028,489 - Pf6N2E2_4310 0.79 -0.3 5,028,489 - Pf6N2E2_4310 0.79 -0.1 5,028,525 - Pf6N2E2_4310 0.81 +0.2 5,028,525 - Pf6N2E2_4310 0.81 +1.0 5,028,534 - Pf6N2E2_4310 0.81 -0.3 5,028,631 - Pf6N2E2_4310 0.84 -0.8 5,028,631 - Pf6N2E2_4310 0.84 +0.9 5,028,631 - Pf6N2E2_4310 0.84 -0.5 5,028,653 + Pf6N2E2_4310 0.84 -0.3 5,028,654 - Pf6N2E2_4310 0.84 +0.2 5,028,654 - Pf6N2E2_4310 0.84 -0.1 5,028,689 + Pf6N2E2_4310 0.85 -0.7 5,028,737 + Pf6N2E2_4310 0.87 -0.9 5,028,738 - Pf6N2E2_4310 0.87 -0.8 5,028,810 + Pf6N2E2_4310 0.89 +0.7 5,028,811 - Pf6N2E2_4310 0.89 +0.3 5,028,811 - Pf6N2E2_4310 0.89 +0.1 5,028,811 - Pf6N2E2_4310 0.89 +0.5 5,028,830 + Pf6N2E2_4310 0.89 -0.2 5,028,830 + Pf6N2E2_4310 0.89 +0.6 5,028,831 - Pf6N2E2_4310 0.89 +0.2 5,028,890 + -0.4 5,028,922 + +1.2 5,028,922 + -0.1 5,028,961 - -3.2 5,029,055 + -2.4 5,029,115 + -2.9 5,029,115 + -1.1 5,029,116 - +0.7 5,029,116 - +0.6 5,029,116 - +1.2 5,029,116 - -0.3 5,029,116 - +0.4 5,029,135 + +0.1 5,029,213 + -0.3 5,029,214 - -1.4 5,029,254 + +0.5 5,029,255 - -0.5 5,029,255 - -0.1 5,029,562 - Pf6N2E2_4311 0.42 -0.4 5,029,562 - Pf6N2E2_4311 0.42 -2.0 5,029,562 - Pf6N2E2_4311 0.42 -0.4 5,029,585 + Pf6N2E2_4311 0.46 -1.5 5,029,585 + Pf6N2E2_4311 0.46 -2.3 5,029,585 + Pf6N2E2_4311 0.46 +0.3 5,029,585 + Pf6N2E2_4311 0.46 +0.5 5,029,585 + Pf6N2E2_4311 0.46 +0.1 5,029,586 - Pf6N2E2_4311 0.46 -0.8 5,029,586 - Pf6N2E2_4311 0.46 -0.0 5,029,586 - Pf6N2E2_4311 0.46 -0.9 5,029,586 - Pf6N2E2_4311 0.46 +0.9 5,029,586 - Pf6N2E2_4311 0.46 +0.6 5,029,780 + Pf6N2E2_4311 0.78 +0.2 5,029,780 + Pf6N2E2_4311 0.78 +0.5 5,029,780 + Pf6N2E2_4311 0.78 -0.5 5,029,780 + Pf6N2E2_4311 0.78 -1.2 5,029,780 + Pf6N2E2_4311 0.78 +0.1 5,029,781 - Pf6N2E2_4311 0.78 +0.3 5,029,941 + -0.9 5,029,941 + +0.7 5,029,941 + -0.3 5,029,941 + -0.4 5,029,942 - -1.3 5,030,011 + Pf6N2E2_4312 0.10 +1.6 5,030,011 + Pf6N2E2_4312 0.10 -0.0 5,030,118 + Pf6N2E2_4312 0.22 +1.4 5,030,119 - Pf6N2E2_4312 0.22 -0.7 5,030,223 - Pf6N2E2_4312 0.33 +0.3 5,030,235 + Pf6N2E2_4312 0.34 -1.0 5,030,235 + Pf6N2E2_4312 0.34 +1.4 5,030,236 - Pf6N2E2_4312 0.35 +2.6 5,030,391 + Pf6N2E2_4312 0.51 +0.3 5,030,392 - Pf6N2E2_4312 0.51 -0.1 5,030,410 + Pf6N2E2_4312 0.53 +0.4 5,030,410 + Pf6N2E2_4312 0.53 -0.5 5,030,410 + Pf6N2E2_4312 0.53 +0.2 5,030,614 - Pf6N2E2_4312 0.75 -0.2 5,030,614 - Pf6N2E2_4312 0.75 -1.4 5,030,634 + Pf6N2E2_4312 0.77 +1.4 5,030,634 + Pf6N2E2_4312 0.77 +1.1 5,030,634 + Pf6N2E2_4312 0.77 -0.1 5,030,635 - Pf6N2E2_4312 0.77 -0.4 5,030,635 - Pf6N2E2_4312 0.77 -0.1 5,030,635 - Pf6N2E2_4312 0.77 -0.2 5,030,635 - Pf6N2E2_4312 0.77 +0.3 5,030,671 + Pf6N2E2_4312 0.81 +0.5 5,030,793 - +1.1 5,030,883 + -0.1 5,030,883 + +0.2 5,030,884 - -0.4 5,030,884 - +0.3 5,030,884 - -0.6
Or see this region's nucleotide sequence