Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1467 and Pf6N2E2_1468 overlap by 4 nucleotides Pf6N2E2_1468 and Pf6N2E2_1469 overlap by 7 nucleotides
Pf6N2E2_1467 - NAD-dependent formate dehydrogenase alpha subunit, at 1,714,851 to 1,717,727
_1467
Pf6N2E2_1468 - NAD-dependent formate dehydrogenase beta subunit, at 1,717,724 to 1,719,283
_1468
Pf6N2E2_1469 - NAD-dependent formate dehydrogenase gamma subunit, at 1,719,277 to 1,719,759
_1469
Position (kb)
1717
1718
1719
1720 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1716.725 kb on - strand, within Pf6N2E2_1467 at 1716.760 kb on + strand, within Pf6N2E2_1467 at 1716.979 kb on + strand, within Pf6N2E2_1467 at 1716.992 kb on + strand, within Pf6N2E2_1467 at 1717.393 kb on + strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.404 kb on + strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.830 kb on + strand at 1717.831 kb on - strand at 1718.085 kb on + strand, within Pf6N2E2_1468 at 1718.311 kb on - strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.483 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.594 kb on - strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.632 kb on - strand, within Pf6N2E2_1468 at 1718.760 kb on + strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.925 kb on + strand, within Pf6N2E2_1468 at 1719.147 kb on + strand at 1719.147 kb on + strand at 1719.147 kb on + strand at 1719.185 kb on + strand at 1719.185 kb on + strand at 1719.185 kb on + strand at 1719.268 kb on + strand at 1719.268 kb on + strand at 1719.399 kb on - strand, within Pf6N2E2_1469 at 1719.531 kb on + strand, within Pf6N2E2_1469 at 1719.566 kb on + strand, within Pf6N2E2_1469 at 1719.567 kb on - strand, within Pf6N2E2_1469 at 1719.647 kb on + strand, within Pf6N2E2_1469 at 1719.648 kb on - strand, within Pf6N2E2_1469 at 1719.648 kb on - strand, within Pf6N2E2_1469 at 1719.678 kb on + strand, within Pf6N2E2_1469 at 1719.679 kb on - strand, within Pf6N2E2_1469 at 1719.759 kb on + strand at 1719.759 kb on + strand at 1719.760 kb on - strand at 1719.760 kb on - strand at 1719.765 kb on + strand at 1719.765 kb on + strand at 1719.766 kb on - strand at 1719.766 kb on - strand at 1720.133 kb on - strand at 1720.133 kb on - strand at 1720.134 kb on + strand at 1720.135 kb on - strand at 1720.135 kb on - strand at 1720.135 kb on - strand at 1720.135 kb on - strand at 1720.136 kb on + strand at 1720.136 kb on + strand at 1720.137 kb on - strand at 1720.137 kb on - strand at 1720.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 1,716,725 - Pf6N2E2_1467 0.65 +1.5 1,716,760 + Pf6N2E2_1467 0.66 -1.6 1,716,979 + Pf6N2E2_1467 0.74 +1.3 1,716,992 + Pf6N2E2_1467 0.74 +0.4 1,717,393 + Pf6N2E2_1467 0.88 +1.3 1,717,394 - Pf6N2E2_1467 0.88 -0.3 1,717,394 - Pf6N2E2_1467 0.88 -0.6 1,717,404 + Pf6N2E2_1467 0.89 +0.3 1,717,405 - Pf6N2E2_1467 0.89 -1.8 1,717,405 - Pf6N2E2_1467 0.89 -1.1 1,717,405 - Pf6N2E2_1467 0.89 -0.5 1,717,830 + +0.9 1,717,831 - -0.6 1,718,085 + Pf6N2E2_1468 0.23 -0.9 1,718,311 - Pf6N2E2_1468 0.38 +0.7 1,718,482 + Pf6N2E2_1468 0.49 +0.8 1,718,482 + Pf6N2E2_1468 0.49 +0.3 1,718,483 - Pf6N2E2_1468 0.49 -2.2 1,718,485 - Pf6N2E2_1468 0.49 -1.0 1,718,485 - Pf6N2E2_1468 0.49 -0.7 1,718,485 - Pf6N2E2_1468 0.49 +0.1 1,718,594 - Pf6N2E2_1468 0.56 -0.7 1,718,625 + Pf6N2E2_1468 0.58 -1.3 1,718,625 + Pf6N2E2_1468 0.58 -0.6 1,718,625 + Pf6N2E2_1468 0.58 -0.4 1,718,625 + Pf6N2E2_1468 0.58 +0.1 1,718,625 + Pf6N2E2_1468 0.58 +0.9 1,718,626 - Pf6N2E2_1468 0.58 -0.1 1,718,626 - Pf6N2E2_1468 0.58 +0.1 1,718,626 - Pf6N2E2_1468 0.58 +2.2 1,718,626 - Pf6N2E2_1468 0.58 -1.1 1,718,631 + Pf6N2E2_1468 0.58 -0.6 1,718,631 + Pf6N2E2_1468 0.58 -1.0 1,718,631 + Pf6N2E2_1468 0.58 +0.1 1,718,632 - Pf6N2E2_1468 0.58 +0.5 1,718,760 + Pf6N2E2_1468 0.66 -1.5 1,718,761 - Pf6N2E2_1468 0.66 -1.3 1,718,761 - Pf6N2E2_1468 0.66 -0.0 1,718,761 - Pf6N2E2_1468 0.66 -0.5 1,718,761 - Pf6N2E2_1468 0.66 +0.1 1,718,761 - Pf6N2E2_1468 0.66 -1.5 1,718,925 + Pf6N2E2_1468 0.77 -2.0 1,719,147 + +0.3 1,719,147 + +0.1 1,719,147 + -0.3 1,719,185 + -0.1 1,719,185 + +0.0 1,719,185 + +0.6 1,719,268 + +0.4 1,719,268 + -0.0 1,719,399 - Pf6N2E2_1469 0.25 +0.1 1,719,531 + Pf6N2E2_1469 0.53 +0.0 1,719,566 + Pf6N2E2_1469 0.60 -0.5 1,719,567 - Pf6N2E2_1469 0.60 +0.1 1,719,647 + Pf6N2E2_1469 0.77 -1.2 1,719,648 - Pf6N2E2_1469 0.77 -0.1 1,719,648 - Pf6N2E2_1469 0.77 -0.6 1,719,678 + Pf6N2E2_1469 0.83 +0.0 1,719,679 - Pf6N2E2_1469 0.83 +1.5 1,719,759 + -1.0 1,719,759 + -1.0 1,719,760 - +0.1 1,719,760 - -0.3 1,719,765 + +0.1 1,719,765 + +0.7 1,719,766 - +1.7 1,719,766 - +0.7 1,720,133 - +0.4 1,720,133 - -0.3 1,720,134 + +1.7 1,720,135 - -0.3 1,720,135 - +0.5 1,720,135 - +0.2 1,720,135 - -0.5 1,720,136 + +1.5 1,720,136 + -0.6 1,720,137 - +0.7 1,720,137 - -0.7 1,720,137 - +0.5
Or see this region's nucleotide sequence