Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23055

Experiment: nitrate (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23045 and BWI76_RS23050 overlap by 4 nucleotidesBWI76_RS23050 and BWI76_RS23055 overlap by 1 nucleotidesBWI76_RS23055 and BWI76_RS23060 overlap by 20 nucleotidesBWI76_RS23060 and BWI76_RS23065 overlap by 8 nucleotides BWI76_RS23045: BWI76_RS23045 - cobalt-precorrin-6A/precorrin-6x reductase, at 4,806,055 to 4,806,834 _RS23045 BWI76_RS23050: BWI76_RS23050 - cobalt-precorrin-3B C(17)-methyltransferase, at 4,806,831 to 4,807,556 _RS23050 BWI76_RS23055: BWI76_RS23055 - cobalamin biosynthesis protein CbiG, at 4,807,556 to 4,808,611 _RS23055 BWI76_RS23060: BWI76_RS23060 - cobalt-precorrin-4 C(11)-methyltransferase, at 4,808,592 to 4,809,365 _RS23060 BWI76_RS23065: BWI76_RS23065 - cobalt-precorrin-6Y C(15)-methyltransferase, at 4,809,358 to 4,809,927 _RS23065 Position (kb) 4807 4808 4809Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4806.613 kb on - strand, within BWI76_RS23045at 4806.836 kb on + strandat 4806.846 kb on - strandat 4806.992 kb on - strand, within BWI76_RS23050at 4807.031 kb on + strand, within BWI76_RS23050at 4807.031 kb on + strand, within BWI76_RS23050at 4807.609 kb on - strandat 4807.785 kb on - strand, within BWI76_RS23055at 4807.839 kb on - strand, within BWI76_RS23055at 4808.209 kb on + strand, within BWI76_RS23055at 4808.633 kb on + strandat 4808.673 kb on - strand, within BWI76_RS23060at 4808.696 kb on + strand, within BWI76_RS23060at 4808.869 kb on + strand, within BWI76_RS23060at 4809.059 kb on - strand, within BWI76_RS23060at 4809.064 kb on - strand, within BWI76_RS23060at 4809.194 kb on - strand, within BWI76_RS23060at 4809.229 kb on + strand, within BWI76_RS23060at 4809.288 kb on - strandat 4809.411 kb on + strandat 4809.467 kb on + strand, within BWI76_RS23065at 4809.531 kb on + strand, within BWI76_RS23065

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate (N)
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4,806,613 - BWI76_RS23045 0.72 -4.0
4,806,836 + +0.3
4,806,846 - -4.3
4,806,992 - BWI76_RS23050 0.22 -3.0
4,807,031 + BWI76_RS23050 0.28 +1.8
4,807,031 + BWI76_RS23050 0.28 +0.4
4,807,609 - -2.6
4,807,785 - BWI76_RS23055 0.22 -4.5
4,807,839 - BWI76_RS23055 0.27 -3.6
4,808,209 + BWI76_RS23055 0.62 -0.3
4,808,633 + +1.4
4,808,673 - BWI76_RS23060 0.10 -4.0
4,808,696 + BWI76_RS23060 0.13 +1.0
4,808,869 + BWI76_RS23060 0.36 -0.9
4,809,059 - BWI76_RS23060 0.60 -3.6
4,809,064 - BWI76_RS23060 0.61 -0.8
4,809,194 - BWI76_RS23060 0.78 -2.1
4,809,229 + BWI76_RS23060 0.82 +1.3
4,809,288 - -2.4
4,809,411 + +1.2
4,809,467 + BWI76_RS23065 0.19 +1.4
4,809,531 + BWI76_RS23065 0.30 +1.7

Or see this region's nucleotide sequence