Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20405 and BWI76_RS20410 are separated by 158 nucleotides BWI76_RS20410 and BWI76_RS20415 are separated by 55 nucleotides BWI76_RS20415 and BWI76_RS20420 are separated by 31 nucleotides BWI76_RS20420 and BWI76_RS20425 overlap by 1 nucleotides
BWI76_RS20405: BWI76_RS20405 - beta-ketoacyl-[acyl-carrier-protein] synthase I, at 4,271,411 to 4,272,628
_RS20405
BWI76_RS20410: BWI76_RS20410 - bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 4,272,787 to 4,274,775
_RS20410
BWI76_RS20415: BWI76_RS20415 - hypothetical protein, at 4,274,831 to 4,275,109
_RS20415
BWI76_RS20420: BWI76_RS20420 - elongation factor P hydroxylase, at 4,275,141 to 4,275,689
_RS20420
BWI76_RS20425: BWI76_RS20425 - hypothetical protein, at 4,275,689 to 4,276,498
_RS20425
Position (kb) 4272
4273
4274
4275 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4273.075 kb on - strand, within BWI76_RS20410 at 4273.129 kb on - strand, within BWI76_RS20410 at 4273.202 kb on + strand, within BWI76_RS20410 at 4273.381 kb on + strand, within BWI76_RS20410 at 4273.742 kb on + strand, within BWI76_RS20410 at 4273.929 kb on + strand, within BWI76_RS20410 at 4274.240 kb on + strand, within BWI76_RS20410 at 4274.443 kb on - strand, within BWI76_RS20410 at 4274.452 kb on + strand, within BWI76_RS20410 at 4274.627 kb on - strand at 4274.885 kb on - strand, within BWI76_RS20415 at 4275.178 kb on - strand at 4275.258 kb on + strand, within BWI76_RS20420
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 4,273,075 - BWI76_RS20410 0.14 +1.1 4,273,129 - BWI76_RS20410 0.17 -0.2 4,273,202 + BWI76_RS20410 0.21 +2.1 4,273,381 + BWI76_RS20410 0.30 +1.0 4,273,742 + BWI76_RS20410 0.48 +0.3 4,273,929 + BWI76_RS20410 0.57 +0.9 4,274,240 + BWI76_RS20410 0.73 +0.1 4,274,443 - BWI76_RS20410 0.83 +0.9 4,274,452 + BWI76_RS20410 0.84 +0.5 4,274,627 - -2.2 4,274,885 - BWI76_RS20415 0.19 -3.1 4,275,178 - +2.3 4,275,258 + BWI76_RS20420 0.21 -3.4
Or see this region's nucleotide sequence