Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20065 and BWI76_RS20070 are separated by 14 nucleotides BWI76_RS20070 and BWI76_RS20075 overlap by 4 nucleotides BWI76_RS20075 and BWI76_RS20080 are separated by 77 nucleotides
BWI76_RS20065: BWI76_RS20065 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,203,569 to 4,204,426
_RS20065
BWI76_RS20070: BWI76_RS20070 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 4,204,441 to 4,205,199
_RS20070
BWI76_RS20075: BWI76_RS20075 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 4,205,196 to 4,206,866
_RS20075
BWI76_RS20080: BWI76_RS20080 - isochorismate synthase MenF, at 4,206,944 to 4,208,269
_RS20080
Position (kb) 4205
4206
4207 Strain fitness (log2 ratio) -2
-1
0
1 at 4204.489 kb on + strand at 4204.777 kb on + strand, within BWI76_RS20070 at 4205.061 kb on + strand, within BWI76_RS20070 at 4205.182 kb on - strand at 4205.899 kb on - strand at 4205.899 kb on - strand, within BWI76_RS20075 at 4206.257 kb on - strand, within BWI76_RS20075 at 4206.268 kb on + strand, within BWI76_RS20075 at 4206.414 kb on + strand, within BWI76_RS20075 at 4206.612 kb on - strand, within BWI76_RS20075 at 4206.761 kb on - strand at 4206.830 kb on + strand at 4206.845 kb on + strand at 4206.904 kb on - strand at 4207.023 kb on - strand at 4207.563 kb on - strand, within BWI76_RS20080 at 4207.664 kb on + strand, within BWI76_RS20080 at 4207.671 kb on + strand, within BWI76_RS20080
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 4,204,489 + +0.1 4,204,777 + BWI76_RS20070 0.44 +1.2 4,205,061 + BWI76_RS20070 0.82 -2.6 4,205,182 - +0.0 4,205,899 - +0.2 4,205,899 - BWI76_RS20075 0.42 -1.0 4,206,257 - BWI76_RS20075 0.63 +0.2 4,206,268 + BWI76_RS20075 0.64 -0.4 4,206,414 + BWI76_RS20075 0.73 -0.6 4,206,612 - BWI76_RS20075 0.85 -0.8 4,206,761 - +1.2 4,206,830 + +1.4 4,206,845 + -1.0 4,206,904 - +0.4 4,207,023 - -0.0 4,207,563 - BWI76_RS20080 0.47 +0.3 4,207,664 + BWI76_RS20080 0.54 -0.8 4,207,671 + BWI76_RS20080 0.55 +1.5
Or see this region's nucleotide sequence