Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12445

Experiment: nitrate (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12440 and BWI76_RS12445 overlap by 10 nucleotidesBWI76_RS12445 and BWI76_RS12450 are separated by 167 nucleotidesBWI76_RS12450 and BWI76_RS12455 are separated by 51 nucleotides BWI76_RS12440: BWI76_RS12440 - MFS transporter, at 2,600,137 to 2,601,528 _RS12440 BWI76_RS12445: BWI76_RS12445 - phage shock protein operon transcriptional activator, at 2,601,519 to 2,602,493 _RS12445 BWI76_RS12450: BWI76_RS12450 - phage shock protein PspA, at 2,602,661 to 2,603,329 _RS12450 BWI76_RS12455: BWI76_RS12455 - envelope stress response membrane protein PspB, at 2,603,381 to 2,603,605 _RS12455 Position (kb) 2601 2602 2603Strain fitness (log2 ratio) -2 -1 0 1at 2600.564 kb on + strand, within BWI76_RS12440at 2600.739 kb on - strand, within BWI76_RS12440at 2600.821 kb on + strand, within BWI76_RS12440at 2600.980 kb on - strand, within BWI76_RS12440at 2601.066 kb on - strand, within BWI76_RS12440at 2601.192 kb on + strand, within BWI76_RS12440at 2601.304 kb on - strand, within BWI76_RS12440at 2601.352 kb on - strand, within BWI76_RS12440at 2601.411 kb on + strandat 2601.442 kb on - strandat 2601.473 kb on + strandat 2601.655 kb on - strand, within BWI76_RS12445at 2601.837 kb on + strand, within BWI76_RS12445at 2601.993 kb on + strand, within BWI76_RS12445at 2602.265 kb on + strand, within BWI76_RS12445at 2602.423 kb on + strandat 2602.826 kb on + strand, within BWI76_RS12450at 2603.123 kb on + strand, within BWI76_RS12450

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate (N)
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2,600,564 + BWI76_RS12440 0.31 -1.5
2,600,739 - BWI76_RS12440 0.43 -2.0
2,600,821 + BWI76_RS12440 0.49 -2.5
2,600,980 - BWI76_RS12440 0.61 -0.2
2,601,066 - BWI76_RS12440 0.67 +0.7
2,601,192 + BWI76_RS12440 0.76 +0.0
2,601,304 - BWI76_RS12440 0.84 +1.3
2,601,352 - BWI76_RS12440 0.87 -0.7
2,601,411 + -0.1
2,601,442 - -0.8
2,601,473 + +0.5
2,601,655 - BWI76_RS12445 0.14 -1.0
2,601,837 + BWI76_RS12445 0.33 -1.3
2,601,993 + BWI76_RS12445 0.49 -0.6
2,602,265 + BWI76_RS12445 0.77 +0.3
2,602,423 + -0.6
2,602,826 + BWI76_RS12450 0.25 +0.2
2,603,123 + BWI76_RS12450 0.69 +1.1

Or see this region's nucleotide sequence