Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08755

Experiment: nitrate (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08745 and BWI76_RS08750 are separated by 104 nucleotidesBWI76_RS08750 and BWI76_RS08755 are separated by 69 nucleotidesBWI76_RS08755 and BWI76_RS08760 are separated by 85 nucleotides BWI76_RS08745: BWI76_RS08745 - proline-specific permease, at 1,843,008 to 1,844,387 _RS08745 BWI76_RS08750: BWI76_RS08750 - kinase inhibitor, at 1,844,492 to 1,844,968 _RS08750 BWI76_RS08755: BWI76_RS08755 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 1,845,038 to 1,846,327 _RS08755 BWI76_RS08760: BWI76_RS08760 - biotin synthase, at 1,846,413 to 1,847,453 _RS08760 Position (kb) 1845 1846 1847Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1844.312 kb on + strandat 1844.312 kb on + strandat 1844.867 kb on - strand, within BWI76_RS08750at 1845.273 kb on - strand, within BWI76_RS08755at 1845.557 kb on + strand, within BWI76_RS08755at 1845.732 kb on + strand, within BWI76_RS08755at 1845.852 kb on - strand, within BWI76_RS08755at 1845.936 kb on + strand, within BWI76_RS08755at 1846.147 kb on + strand, within BWI76_RS08755at 1846.183 kb on - strand, within BWI76_RS08755at 1846.408 kb on + strandat 1846.536 kb on - strand, within BWI76_RS08760at 1846.653 kb on + strand, within BWI76_RS08760at 1846.747 kb on + strand, within BWI76_RS08760at 1846.872 kb on + strand, within BWI76_RS08760at 1846.937 kb on - strand, within BWI76_RS08760

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate (N)
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1,844,312 + +0.1
1,844,312 + -0.4
1,844,867 - BWI76_RS08750 0.79 -0.2
1,845,273 - BWI76_RS08755 0.18 -4.0
1,845,557 + BWI76_RS08755 0.40 -0.2
1,845,732 + BWI76_RS08755 0.54 -2.2
1,845,852 - BWI76_RS08755 0.63 -0.0
1,845,936 + BWI76_RS08755 0.70 -1.4
1,846,147 + BWI76_RS08755 0.86 -1.5
1,846,183 - BWI76_RS08755 0.89 -4.3
1,846,408 + +1.4
1,846,536 - BWI76_RS08760 0.12 -0.1
1,846,653 + BWI76_RS08760 0.23 -0.1
1,846,747 + BWI76_RS08760 0.32 +1.2
1,846,872 + BWI76_RS08760 0.44 +2.2
1,846,937 - BWI76_RS08760 0.50 +0.1

Or see this region's nucleotide sequence